Abstract:
:A crude preparation of heavy sarcoplasmic reticulum (HSR) was isolated using 1 gram of muscle obtained from swine susceptible to malignant hyperthermia (MH) and from control swine. The caffeine and ATP concentration-dependency of Ca-release was determined using suction filtration with radioisotopic 45Ca as a tracer. Rates of release were determined using a rapid filtration system. Caffeine and ATP-induced Ca-release from MH-susceptible (MHS) HSR occurred at one-tenth the concentration of agonist that was required for control muscle HSR. No differences in rates and amounts of release were observed when agonist concentrations were used that caused maximum release for controls. However, at the threshold concentration of caffeine causing release for control HSR, the MHS HSR released 4-times as much Ca and at 3-times the rate of controls. These findings indicate that increased rates and amounts of Ca-release are due to the hypersensitivity of the Ca-release channel of HSR and that this abnormality can be detected using 1 gram of muscle.
journal_name
Mol Cell Biochemjournal_title
Molecular and cellular biochemistryauthors
O'Brien PJdoi
10.1007/BF00223492subject
Has Abstractpub_date
1990-03-05 00:00:00pages
53-9issue
1eissn
0300-8177issn
1573-4919journal_volume
93pub_type
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