Abstract：
:Glioblastomas are the most malignant and most frequent brain tumors and exciting targets of gene and immunotherapy. Despite rapid development of experimental therapy little is known about the cellular behaviour of therapeutic oligodeoxynucleotides (ODNs). Here we designed uptake, cellular distribution and cellular binding proteins of immunostimulatory CpG-ODNs in glioblastoma cells by flow cytometry, fluorescence microscopy and mass spectrometry. Our data show that the phosphorothioate (PS) CpG-ODNs uptake in T98G and C6 cells is dose-, time-, temperature-dependent and independent of the CpG dinucleotides. Uptake can be inhibited by sodium azide, polyanions but not by chloroquine. After internalisation FITC labelled CpG-ODNs showed a spotted distribution in cytoplasm. Dozens of cellular binding proteins were identified using mass spectrometry. The binding of ODNs to proteins is dependent on modification and sequence but independent on CpG motif. ODNs bind to cellular proteins that are important for RNA processing and transport. Furthermore, three novel membrane proteins were identified, which might contribute to uptake of ODNs. ODNs binding to these proteins might interfere with the physiological function and thus might cause unwanted effects. Such binding also might influence the uptake efficiency or cellular distribution of therapeutic ODNs.
journal_name
Mol Cell Biochemjournal_title
Molecular and cellular biochemistryauthors
Zhang Z,Weinschenk T,Schluesener HJdoi
10.1007/s11010-005-6605-0keywords:
subject
Has Abstractpub_date
2005-04-01 00:00:00pages
35-46issue
1-2eissn
0300-8177issn
1573-4919journal_volume
272pub_type
杂志文章abstract：:To evaluate the effects of the in vivo endotoxin treatment of the rat on (1) the contractile responses in the subsequently isolated papillary muscle to adrenergic and cholinergic agonists and (2) the biochemical parameters (cyclic GMP, nitric oxide synthesis, protein phosphorylation and ADP-ribosyslation) in the subse...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/BF00408671
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abstract：:Diabetes-induced changes in glucose formation, intracellular and mitochondrial glutathione redox states as well as hydroxyl free radicals (HFR) generation have been investigated in rabbit kidney-cortex tubules. In contrast to renal tubules of control animals, diabetes-evoked increase in glucose formation in the presen...
journal_title：Molecular and cellular biochemistry
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pub_type： 杂志文章,评审
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journal_title：Molecular and cellular biochemistry
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更新日期：1998-01-01 00:00:00
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更新日期：2010-06-01 00:00:00
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doi：10.1007/s11010-017-3034-9
更新日期：2017-10-01 00:00:00
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journal_title：Molecular and cellular biochemistry
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doi：
更新日期：1995-11-08 00:00:00
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pub_type： 杂志文章
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更新日期：2011-01-01 00:00:00
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1023/a:1007040708579
更新日期：2000-02-01 00:00:00
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/BF00240278
更新日期：1978-11-01 00:00:00
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更新日期：2011-06-01 00:00:00
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更新日期：2016-10-01 00:00:00
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更新日期：1990-03-05 00:00:00
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更新日期：2014-07-01 00:00:00
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