当前位置： SCI文献检索 > MOLECULAR AND CELLULAR BIOCHEMISTRY期刊下所有文献 > HepaCAM inhibits clear cell renal carcinoma 786-0 cell proliferation via blocking PKCε translocation from cytoplasm to plasma membrane.

HepaCAM inhibits clear cell renal carcinoma 786-0 cell proliferation via blocking PKCε translocation from cytoplasm to plasma membrane.

Abstract：

:Hepatocyte cell adhesion molecule (HepaCAM) plays a crucial role in tumor progression and has been recognized as a novel tumor suppressor gene. The high protein expression level of protein kinase Cε (PKCε) has been discovered in many tumor types. In the present study, we determined HepaCAM and PKCε protein levels in human clear cell renal cell carcinoma (ccRCC) tissues and analyzed the correlation between them. We observed an inverse relationship in the expression of HepaCAM and PKCε in ccRCC and adjacent normal tissues. In ccRCC tissue, HepaCAM expression was undetectable while PKCε expression was high; the opposite was found in the adjacent normal tissue. Western blot analysis demonstrated that PKCε cytosolic protein levels increased while plasma membrane protein levels decreased without any change in total protein following infection of the ccRCC cell line 786-0 with adenovirus-GFP-HepaCAM (Ad-GFP-HepaCAM). Moreover, the application of Ad-GFP-HepaCAM combined with a PKCε-specific translocation inhibitor (εV1-2) effectively inhibited 786-0 cell growth. Ad-mediated expression of HepaCAM in 786-0 cells reduced the levels of phosphorylated AKT and cyclin D1 and inhibited cell proliferation. In summary, our studies point to interesting connections between HepaCAM and PKCε in tissues and in vitro. HepaCAM may prevent the translocation of PKCε from cytosolic to particulate fractions, resulting in the inhibition of 786-0 cell proliferation. Therapeutic manipulation of these novel protein targets may provide new ways of treating ccRCC.

journal_name

Mol Cell Biochem

journal_title

Molecular and cellular biochemistry

authors

Tan B,Tan J,Du H,Quan Z,Xu X,Jiang X,Luo C,Wu X

doi

10.1007/s11010-014-1991-9

subject

Has Abstract

pub_date

2014-06-01 00:00:00

pages

95-102

issue

1-2

eissn

0300-8177

issn

1573-4919

journal_volume

391

pub_type

杂志文章

相关文献

MOLECULAR AND CELLULAR BIOCHEMISTRY文献大全
  • Effect of different carbon sources on the biosynthesis of polyunsaturated fatty acids of alpha-linolenic acid family in culture of minimal deviation hepatoma 7288 C cells.

    abstract：:The effect of three different carbon sources on the biosynthesis of polyunsaturated fatty acids of the alpha-linolenic acid series was investigated in hepatoma tissue culture (HTC) cells. Alpha linolenic acid was converted to higher homologs by a desaturating route that synthetized mainly 18:4 (delta6, 9, 12, 15), 20:...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/BF01731553

    authors： de Alaniz MJ,Brenner RR

    更新日期：1976-08-30 00:00:00

  • Muscle fiber contractile type influences the regulation of mitochondrial function.

    abstract：:Mitochondrial respiratory rates and regulation by phosphate acceptors were studied on permeabilized fiber bundles differing in their myosin heavy chain profiles. The acceptor control ratio, an indicator of oxidation to phosphorylation coupling, and mitochondrial K(m) for ADP were the highest in type I, intermediate in...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-005-2464-y

    authors： Gueguen N,Lefaucheur L,Fillaut M,Herpin P

    更新日期：2005-08-01 00:00:00

  • Antioxidants and vision health: facts and fiction.

    abstract：:A number of nutritional supplements containing antioxidants are advertised for better vision health. Do they benefit the average consumer? The literature was examined for the effectiveness of antioxidants for human eye health, and for the intricacies in collection of such evidence. The following diseases were consider...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章,评审

    doi：10.1007/s11010-013-1908-z

    authors： Grover AK,Samson SE

    更新日期：2014-03-01 00:00:00

  • Refolding of soluble leukemia inhibitory factor receptor fusion protein (gp 190 sol DAF) from urea.

    abstract：:The insoluble inclusion bodies of soluble leukemia inhibitory factor receptor fusion protein (gp 190 sol DAF) was solubilized in 8 M urea on the unfolding transitions, and several factors on the aggregate formation were indirectly analyzed for the refolding of gp 190 sol DAF. Results indicate that the refolding yield ...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1023/a:1006890627639

    authors： Liu H,Moreau JF,Gualde N,Fu J

    更新日期：1997-04-01 00:00:00

  • Protein phosphorylation in rat cardiac microsomes: effects of inhibitors of protein kinase A and of phosphatases.

    abstract：:The phosphorylation of rat cardiac microsomal proteins was investigated with special attention to the effects of okadaic acid (an inhibitor of protein phosphatases), inhibitor 2 of protein phosphatase 1 and inhibitor of cyclic AMP-dependent protein kinase (protein kinase A). The results showed that okadaic acid (5 mic...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1023/a:1006879427457

    authors： Sulakhe PV,Vo XT,Morris TE,Pato MD,Khandelwal RL

    更新日期：1997-10-01 00:00:00

  • The tyrosine kinase inhibitor genistein increases basal cAMP and potentiates forskolin-induced cAMP accumulation in A549 human airway epithelial cells.

    abstract：:Genistein is often used as an inhibitor of tyrosine kinases. A less studied side effect of genistein is an inhibition of cyclic AMP-phosphodiesterase (cAMP-PDE) activity resulting in increased cAMP accumulation. The effect of genistein on intracellular cAMP-levels, basal and forskolin-induced, was studied in A549 huma...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1023/a:1020655724911

    authors： Burvall KM,Palmberg L,Larsson K

    更新日期：2002-11-01 00:00:00

  • Novel classes of fatty acid and retinol binding protein from nematodes.

    abstract：:Parasitic nematodes have recently been found to produce proteins which represent two new classes of fatty acid and retinoid binding protein. The first is the nematode polyprotein allergens/antigens (NPAs) which, as their name suggests, are synthesised as large polyproteins which are subsequently cleaved at regularly s...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章,评审

    doi：

    authors： McDermott L,Cooper A,Kennedy MW

    更新日期：1999-02-01 00:00:00

  • Kynurenic acid downregulates IL-17/1L-23 axis in vitro.

    abstract：:Exploring the function of interleukin (IL) 17 and related cytokine interactions have been proven useful toward understanding the role of inflammation in autoimmune diseases. Production of the inflammatory cytokine IL-23 by dendritic cells (DC's) has been shown to promote IL-17 expression by Th17 cells. It is well esta...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-017-2975-3

    authors： Salimi Elizei S,Poormasjedi-Meibod MS,Wang X,Kheirandish M,Ghahary A

    更新日期：2017-07-01 00:00:00

  • Efficient secretion of biologically active Chondroitinase ABC from mammalian cells in the absence of an N-terminal signal peptide.

    abstract：:Proteoglycans carrying chondroitin sulfate side chains have been shown to fulfill important biological functions in development, disease, and signaling. One area of considerable interest is the functional importance of chondroitin sulfates as inhibitors of the regeneration of axonal projections in the mammalian centra...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-010-0705-1

    authors： Klüppel M

    更新日期：2011-05-01 00:00:00

  • Fenofibrate but not fenofibric acid inhibits 11beta-hydroxysteroid dehydrogenase 1 in C2C12 myotubes.

    abstract：:Fibrates and WY-14643 are widely used as lipid-lowering drugs via modulation of peroxisome proliferator-activated receptor alpha (PPAR α) for the treatment of a wide range of metabolic disorders. Here, to address the question whether PPAR α agonists can affect the enzymatic activity of 11beta-hydroxysteroid dehydrogen...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-010-0532-4

    authors： Kim CH,Ramu R,Ahn JH,Bae MA,Cho YS

    更新日期：2010-11-01 00:00:00

  • Study on lipid peroxidation potential in different tissues induced by ascorbate-Fe2+: possible factors involved in their differential susceptibility.

    abstract：:Susceptibility of four major rat tissues to oxidative damage in terms of lipid peroxidation induced by in vitro by ascorbate-Fe2+ in homogenates and mitochondria has been examined. Lipid peroxidation, as assessed by thiobarbituric acid reactive substances (TBARS) and conjugated dienes was maximum in brain followed by ...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1023/a:1006895531504

    authors： Pushpendran CK,Subramanian M,Devasagayam TP,Singh BB

    更新日期：1998-01-01 00:00:00

  • Growth factor activation in myocardial vascularization: therapeutic implications.

    abstract：:A rapid growth of the coronary vasculature occurs during prenatal and early postnatal periods as precursor cells from the epi- and sub-epicardium differentiate, migrate and form vascular structures (vasculogenesis) which then fuse, branch and in some cases recruit cells to form three tunics (angiogenesis). These proce...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章,评审

    doi：10.1023/b:mcbi.0000044369.88528.a3

    authors： Tomanek RJ,Zheng W,Yue X

    更新日期：2004-09-01 00:00:00

  • Nifedipine administration impairs natural resistance of mice to Salmonella typhimurium.

    abstract：:Administration of Ca2+ channel blockers in cardiac disorders and the central role of Ca2+ in modulating neutrophil functions, prompted us to investigate whether administration of nifedipine to mice would alter their natural resistance to infectious agents like Salmonella typhimurium. Neutrophil chemiluminescence (CL) ...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/BF00219971

    authors： Nalini K,Andrabi KI,Ganguly NK,Wahi PL

    更新日期：1990-06-25 00:00:00

  • Modification of myosin isozymes and SR Ca(2+)-pump ATPase of the diabetic rat heart by lipid-lowering interventions.

    abstract：:To define metabolic influences on cardiac myosin expression and sarcoplasmic reticulum (SR) Ca(2+)-stimulated ATPase streptozotocin-diabetic rats were treated for 9-10 wk with etomoxir, an inhibitor of carnitine palmitoyl transferase I (CPT-1) and fatty acid synthesis, or an antilipolytic drug, acipimox. Etomoxir redu...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/BF00925676

    authors： Rupp H,Elimban V,Dhalla NS

    更新日期：1994-03-16 00:00:00

  • Activation of STING inhibits cervical cancer tumor growth through enhancing the anti-tumor immune response.

    abstract：:Cervical cancer remains the second leading cause of gynecologic cancer-related mortality among women worldwide. STING (stimulator of interferon genes) was reported to be involved in the immune surveillance of tumors. However, the specific role of STING in cervical cancer remains unclear. In this study, we found that t...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-020-03967-5

    authors： Shi F,Su J,Wang J,Liu Z,Wang T

    更新日期：2020-11-03 00:00:00

  • A role for guanidino compounds in the brain.

    abstract：:Guanidino compounds of guanidinoethanesulfonic acid, guanidinoacetic acid, guanidinosuccinic acid, N-acetylarginine, beta-guanidinopropionic acid, creatinine, gamma-guanidinobutyric acid, arginine, guanidine, methylguanidine, homoarginine and alpha-guanidinoglutaric acid are present in the mammalian brain. These guani...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章,评审

    doi：

    authors： Hiramatsu M

    更新日期：2003-02-01 00:00:00

  • The activity of erythrocyte and brain Na+/K+ and Mg2+-ATPases in rats subjected to acute homocysteine and homocysteine thiolactone administration.

    abstract：:Hyperhomocysteinemia is associated with various pathologies including cardiovascular disease, stroke, and cognitive dysfunctions. Systemic administration of homocysteine can trigger seizures in animals, and patients with homocystinuria suffer from epileptic seizures. Available data suggest that homocysteine can be har...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-009-0040-6

    authors： Rasić-Marković A,Stanojlović O,Hrncić D,Krstić D,Colović M,Susić V,Radosavljević T,Djuric D

    更新日期：2009-07-01 00:00:00

  • An alpha subunit-deficient form of eukaryotic protein synthesis initiation factor eIF-2 from rabbit reticulocyte lysate and its activity in ternary complex formation.

    abstract：:Eukaryotic protein synthesis initiation factor eIF-2 is usually isolated as a heterotrimer (alphabeta gamma). By use of Sephacryl S-300 fractionation an alpha subunit-deficient form of eIF-2 was identified in impure preparations from rabbit reticulocyte lysate and it appeared in these preparations to be still active i...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1023/a:1006829615464

    authors： Mouat MF,Manchester K

    更新日期：1998-06-01 00:00:00

  • Evidence for HSP70-like protein in the RBC membrane of the hereditarily anemic Belgrade laboratory (b/b) rat.

    abstract：:We have demonstrated that in normal and b/b rat red blood cells (RBCs) hsp70-like protein (heat shock protein 70-like) is localized in the cytosol and it is exported via exosomes during in vivo reticulocytes maturation. As we have presumed, in the mutant (b/b) rat, hsp70-like protein transfers from cytosol to the RBC ...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1023/a:1006877026611

    authors： Zarić J,Glisin V,Popović Z

    更新日期：1998-01-01 00:00:00

  • Purification and characterization of tripeptide aminopeptidase from bovine dental follicles.

    abstract：:Tripeptide aminopeptidase (EC 3.4.11.4) was purified from bovine dental follicles by a series of chromatographies. Purified enzyme had a specific activity of 59.5 units per mg protein with L-prolyl-glycylglycine as substrate. The pH optimum was 8.0. The purified native enzyme had a Mr of 230,000 and was shown to be a ...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/BF00926579

    authors： Hiraoka BY,Harada M

    更新日期：1993-12-08 00:00:00

  • Modulation of antioxidant enzyme activities, platelet aggregation and serum prostaglandins in rats fed spray-dried milk containing n-3 fatty acid.

    abstract：:Spray-dried milk enriched with n-3 fatty acids from linseed oil or fish oil were fed to rats to study its influence on liver lipid peroxides, hepatic antioxidant enzyme activities, serum prostaglandins and platelet aggregation. Significant level of alpha linolenic acid, eicosapentaenoic acid and docosahexaenoic acid w...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-005-2324-9

    authors： Ramaprasad TR,Baskaran V,Krishnakantha TP,Lokesh BR

    更新日期：2005-12-01 00:00:00

  • Involvement of PARP and poly(ADP-ribosyl)ation in the early stages of apoptosis and DNA replication.

    abstract：:We have focused on the roles of PARP and poly(ADP-ribosyl)ation early in apoptosis, as well as during the early stages of differentiation-linked DNA replication. In both nuclear processes, a transient burst of PAR synthesis and PARP expression occurs early, prior to internucleosomal DNA cleavage before commitment to a...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：

    authors： Simbulan-Rosenthal CM,Rosenthal DS,Iyer S,Boulares H,Smulson ME

    更新日期：1999-03-01 00:00:00

  • Genomic organization and neonatal expression of the bovine myostatin gene.

    abstract：:Myostatin belongs to the Transforming Growth Factor-beta (TGF-beta) superfamily and is expressed in developing and mature skeletal muscle. Biologically, the role of myostatin seems to be extremely well conserved during evolution since inactivating mutations in myostatin gene cause similar phenotype of heavy muscling i...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1023/a:1010801511963

    authors： Jeanplong F,Sharma M,Somers WG,Bass JJ,Kambadur R

    更新日期：2001-04-01 00:00:00

  • Expression of the mRNA encoding truncated PPAR alpha does not correlate with hepatic insensitivity to peroxisome proliferators.

    abstract：:Two alternatively spliced forms of human PPAR alpha mRNA, PPAR alpha1 and PPAR alpha2, have been identified. PPAR alpha1 mRNA gives rise to an active PPAR alpha protein while PPAR alpha2 mRNA gives rise to a form of PPAR which lacks the ligand-binding domain. PPAR alpha2 is unable to activate a peroxisome proliferator...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1023/a:1007248007372

    authors： Hanselman JC,Vartanian MA,Koester BP,Gray SA,Essenburg AD,Rea TJ,Bisgaier CL,Pape ME

    更新日期：2001-01-01 00:00:00

  • Kinetic characterization of Zinc transport process and its inhibition by Cadmium in isolated rat renal basolateral membrane vesicles: in vitro and in vivo studies.

    abstract：:We firstly characterized zinc uptake phenomenon across basolateral membrane vesicles (BLMVs) isolated from normal rat kidney. The process was found to be time, temperature, and substrate concentration dependent, and displayed saturability. Zn(2+) uptake was competitively inhibited in the presence of 2 mM Cd with Ki of...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-006-2676-9

    authors： Kaur J,Sharma N,Attri S,Gogia L,Prasad R

    更新日期：2006-02-01 00:00:00

  • Effect of pentoxifylline on arachidonic acid metabolism, neutral lipid synthesis and accumulation during induction of the lipocyte phenotype by retinol in murine hepatic stellate cell.

    abstract：:In liver fibrosis, the quiescent hepatic stellate cells (HSC) are activated to proliferate and express the activated myofibroblast phenotype, losing fat droplets and the stored vitamin A, and depositing more extracellular matrix. Therapeutic strategies for liver fibrosis are focused on HSC. Pentoxifylline (PTF), an an...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1023/a:1027356412399

    authors： Cardoso CC,Paviani ER,Cruz LA,Guma FC,Borojevic R,Guaragna RM

    更新日期：2003-12-01 00:00:00

  • Casein kinase II in signal transduction and cell cycle regulation.

    abstract：:Casein kinase II is a protein serine/threonine kinase that is ubiquitously distributed in eukaryotes. Molecular cloning studies and protein sequence analysis of purified proteins have demonstrated the existence of two related, but distinct, isoenzymic forms of its catalytic subunit in mammals and birds. At present, th...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章,评审

    doi：10.1007/BF01076770

    authors： Litchfield DW,Lüscher B

    更新日期：1993-11-01 00:00:00

  • Lupeol and its ester inhibit alteration of myocardial permeability in cyclophosphamide administered rats.

    abstract：:Cyclophosphamide (CP), an alkylating agent widely used in cancer chemotherapy causes cardiac membrane damage. Lupeol, a pentacyclic triterpene, isolated from Crataeva nurvala stem bark and its ester, lupeol linoleate possess a wide range of medicinal properties. The effect of lupeol and its ester was evaluated in CP i...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-006-9171-1

    authors： Sudharsan PT,Mythili Y,Selvakumar E,Varalakshmi P

    更新日期：2006-11-01 00:00:00

  • Interactions between subunits of protein kinase CK2 and their protein substrates influences its sensitivity to specific inhibitors.

    abstract：:Five isoforms of CK2 may exist simultaneously in yeast cells: free catalytic subunits CK2α', CK2α and three holoenzymatic structures composed of αα'ββ', α(2)ββ' and α'(2)ββ'. Each isolated and purified form exhibits properties typical for CK2, but they differ in substrate specificity as well as in sensitivity to speci...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-011-0951-x

    authors： Janeczko M,Masłyk M,Szyszka R,Baier A

    更新日期：2011-10-01 00:00:00

  • Involvement of GPR12 in the regulation of cell proliferation and survival.

    abstract：:GPR12, a member of the orphan G-protein-coupled receptor family, constitutively activates the Gs protein and increases intracellular cyclic AMP concentrations. GPR12 can be activated by its known ligand-sphingosylphosphorylcholine, which regulates cellular physiological activities, including proliferation, neurite ext...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-012-1287-x

    authors： Lu X,Zhang N,Meng B,Dong S,Hu Y

    更新日期：2012-07-01 00:00:00