Abstract:
:Cervical cancer remains the second leading cause of gynecologic cancer-related mortality among women worldwide. STING (stimulator of interferon genes) was reported to be involved in the immune surveillance of tumors. However, the specific role of STING in cervical cancer remains unclear. In this study, we found that the cGAS (Cyclic GMP-AMP synthase)/STING signal decreased in cervical cancer cells. Knockdown of STING by siRNA enhanced the cell viability and migration of cervical cancer cells, while activation of STING by ADU-S100 inhibited the cell viability of cervical cancer cells, with no effect on the migration and apoptosis. In addition, ADU-S100 promoted the secretion of IFNβ and IL-6, and the activation of TBK1 (TANK-binding kinase 1)/NF-κB (nuclear factor kappa-B) pathway. Meanwhile, knockdown of STING inhibited the production of IFNβ and IL-6 that were triggered by dsDNA and suppressed the TBK1/NF-κB signaling. ADU-S100 also suppressed tumor growth in vivo and increased the tumor-infiltrating CD8+ T cell and CD103+ dendritic cell numbers. The NF-κB signal inhibitor limited the increasing numbers of CD8+ T cell and CD103+ dendritic cells induced by ADU-S100, without influence on tumor growth. Hence, our study suggested that STING could serve as a potential novel immunotherapeutic target for cervical cancer.
journal_name
Mol Cell Biochemjournal_title
Molecular and cellular biochemistryauthors
Shi F,Su J,Wang J,Liu Z,Wang Tdoi
10.1007/s11010-020-03967-5subject
Has Abstractpub_date
2020-11-03 00:00:00eissn
0300-8177issn
1573-4919pii
10.1007/s11010-020-03967-5pub_type
杂志文章abstract::The creatine kinase (CK) system is essential for cellular energetics in tissues or cells with high and fluctuating energy requirements. Creatine itself is known to protect cells from stress-induced injury. By using an siRNA approach to silence the CK isoenzymes in human keratinocyte HaCaT cells, expressing low levels ...
journal_title:Molecular and cellular biochemistry
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journal_title:Molecular and cellular biochemistry
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journal_title:Molecular and cellular biochemistry
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journal_title:Molecular and cellular biochemistry
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journal_title:Molecular and cellular biochemistry
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journal_title:Molecular and cellular biochemistry
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更新日期:2002-05-01 00:00:00