Abstract:
:Porcine reproductive and respiratory syndrome (PRRS) is one of the most economically important diseases of swine, which is caused by PRRS virus (PRRSV). CD151, one of PRRSV entry mediators, determines the cell susceptibility for PRRSV. Emerging evidence indicates that the host microRNAs (miRNAs) play key roles in modulating virus infection and viral pathogenesis. In the present study, targeting porcine CD151 miRNAs were identified, and their function during PRRSV infection in MARC-145 cells was further verified. We found that miR-506 could directly target porcine CD151 3'-UTR mRNA by luciferase reporter assay. Overexpression of miR-506 significantly decreased CD151 expression at both mRNA and protein levels. Furthermore, overexpression of miR-506 reduced cellular PRRSV replication and virus release in MARC-145 cells. Our results suggested that miR-506 could inhibit PRRSV replication by directly targeting PRRSV receptor of CD151 in MARC-145 cells. However, the molecular mechanisms of miR-506 and its function in vivo need further investigation.
journal_name
Mol Cell Biochemjournal_title
Molecular and cellular biochemistryauthors
Wu J,Peng X,Zhou A,Qiao M,Wu H,Xiao H,Liu G,Zheng X,Zhang S,Mei Sdoi
10.1007/s11010-014-2103-6subject
Has Abstractpub_date
2014-09-01 00:00:00pages
275-81issue
1-2eissn
0300-8177issn
1573-4919journal_volume
394pub_type
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