Modulation of antioxidant enzyme activities, platelet aggregation and serum prostaglandins in rats fed spray-dried milk containing n-3 fatty acid.


:Spray-dried milk enriched with n-3 fatty acids from linseed oil or fish oil were fed to rats to study its influence on liver lipid peroxides, hepatic antioxidant enzyme activities, serum prostaglandins and platelet aggregation. Significant level of alpha linolenic acid, eicosapentaenoic acid and docosahexaenoic acid were accumulated at the expense of arachidonic acid in the liver of rats fed n-3 fatty acid enriched formulation. The linseed oil and fish oil enriched formulation fed group had 44 and 112% higher level of lipid peroxides in liver homogenate compared to control rats fed groundnut oil enriched formulation. Catalase activity in liver homogenate was increased by 37 and 183% respectively in linseed oil and fish oil formulation fed rats. The glutathione peroxidase activity decreased to an extent of 25-36% and glutathione transferase activity increased to an extent of 34-39% in rats fed n-3 fatty acids enriched formulation. Feeding n-3 fatty acid enriched formulation significantly elevated the n-3 fatty acids in platelets and increased the lipid peroxide level to an extent of 4.2-4.5 fold compared to control. The serum thromboxane B2 level was decreased by 35 and 42% respectively in linseed oil and fish oil enriched formulation fed rats, whereas, 6-keto- prostaglandin F1alpha level was decreased by 17 and 23% respectively in linseed oil and fish oil enriched formulation fed rats. The extent and rate of platelet aggregation was decreased significantly in n-3 fatty acids enriched formulation fed rats. This indicated that n-3 fatty acids enriched formulation beneficially reduces platelet aggregation and also enhances the activities of hepatic antioxidant enzymes such as catalase and glutathione transferase.


Mol Cell Biochem


Ramaprasad TR,Baskaran V,Krishnakantha TP,Lokesh BR





Has Abstract


2005-12-01 00:00:00












  • Typical phenotypic spectrum of velocardiofacial syndrome occurs independently of deletion size in chromosome 22q11.2.

    abstract::Velocardiofacial syndrome (VCFS) is a relatively common developmental disorder characterized by craniofacial anomalies and conotruncal heart defects. Many VCFS patients present hemizygous deletions on part of chromosome 22q11.2; suggestive that haploinsufficiency in this region is responsible for this etiology. Most 2...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Sandrin-Garcia P,Abramides DV,Martelli LR,Ramos ES,Richieri-Costa A,Passos GA

    更新日期:2007-09-01 00:00:00

  • Culturing of human vascular endothelial cells strongly affects their endothelin-1 and prostacyclin production.

    abstract::Cultured human umbilical vein endothelial cells (HUVECs) are a widely used model to study the regulation of endothelial production of vasoactive substances such as endothelin-1 (ET-1) and prostacyclin (PGI2) in human. As even short term culturing is known to affect the function of many cell types, we studied whether t...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Ranta V,Orpana A,Mikkola T,Ylikorkala O,Viinikka L

    更新日期:1997-12-01 00:00:00

  • Inhibition of cytosolic and mitochondrial creatine kinase by siRNA in HaCaT- and HeLaS3-cells affects cell viability and mitochondrial morphology.

    abstract::The creatine kinase (CK) system is essential for cellular energetics in tissues or cells with high and fluctuating energy requirements. Creatine itself is known to protect cells from stress-induced injury. By using an siRNA approach to silence the CK isoenzymes in human keratinocyte HaCaT cells, expressing low levels ...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Lenz H,Schmidt M,Welge V,Kueper T,Schlattner U,Wallimann T,Elsässer HP,Wittern KP,Wenck H,Staeb F,Blatt T

    更新日期:2007-12-01 00:00:00

  • Effect of albumin on acyl-CoA: lysolecithin acyltransferase, lysolecithin: lysolecithin acyltransferase and acyl-CoA hydrolase from rabbit lung.

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    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Pérez-Gil J,Estrada P,Acebal C,Arche R

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  • High altitude hypoxia: an intricate interplay of oxygen responsive macroevents and micromolecules.

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    pub_type: 杂志文章,评审


    authors: Sarkar S,Banerjee PK,Selvamurthy W

    更新日期:2003-11-01 00:00:00

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    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Ranzato E,Patrone M,Pedrazzi M,Burlando B

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  • Sequential alterations in the micro-localization of catalase in mouse liver after treatment with hypolipidemic drugs.

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    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


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    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


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  • Telopodes of telocytes are influenced in vitro by redox conditions and ageing.

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    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Enciu AM,Popescu LM

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    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章,评审


    authors: Sabri A,Steinberg SF

    更新日期:2003-09-01 00:00:00

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    pub_type: 杂志文章


    authors: Hong Z,Hong Z,Wu D,Nie H

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    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


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  • Endocan, a potential prognostic and diagnostic biomarker of acute leukemia.

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    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Xu Z,Zhang S,Zhou Q,Wang Y,Xia R

    更新日期:2014-10-01 00:00:00

  • Alteration of antioxidant status following sympathectomy: differential effects of modified plasma levels of adrenaline and noradrenaline.

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    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Toleikis PM,Godin DV

    更新日期:1995-11-08 00:00:00

  • Experimental evidence for the therapeutic potential of tempol in the treatment of acute liver injury.

    abstract::Oxidative stress is one of the mechanisms involved in the acute carbon tetrachloride (CCl4)-induced hepatotoxicity. Since 4-hydroxy-2,2,6,6-tetramethylpiperidin-1-oxyl, known as tempol, has powerful antioxidant properties, we investigated its potential hepatoprotective effects and the underlying mechanisms that may ad...

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    pub_type: 杂志文章


    authors: Abouzied MM,Eltahir HM,Taye A,Abdelrahman MS

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    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


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  • Characteristics of an ADP receptor mediating platelet activation.

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    pub_type: 杂志文章,评审


    authors: Colman RW,Figures WR

    更新日期:1984-01-01 00:00:00

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    pub_type: 杂志文章


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    pub_type: 杂志文章


    authors: Mukhopadhyay-Sardar S,Rana MP,Chatterjee M

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    abstract::Production of human papillomavirus type 16 major capsid protein L1 in undifferentiated cells is negatively regulated by several yet unidentified cis-acting inhibitory RNA elements, among which a major element is located within the first 514 nucleotides of the L1-mRNA. By Northern blotting we examined effect of the maj...

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    pub_type: 杂志文章


    authors: Mori S,Ozaki S,Yasugi T,Yoshikawa H,Taketani Y,Kanda T

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    authors: Sharma M,Kumar R,Singh R,Kaur J

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    authors: Egom EE,Bae JS,Capel R,Richards M,Ke Y,Pharithi RB,Maher V,Kruzliak P,Lei M

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    pub_type: 杂志文章


    authors: Lv L,Xiao XY,Gu ZH,Zeng FQ,Huang LQ,Jiang GS

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    pub_type: 杂志文章,评审


    authors: Paule MR,Bateman E,Hoffman L,Iida C,Imboden M,Kubaska W,Kownin P,Li H,Lofquist A,Risi P

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    authors: Sugano M,Tsuchida K,Makino N

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    pub_type: 杂志文章


    authors: Wang FZ,Fei HR,Li XQ,Shi R,Wang de C

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    pub_type: 杂志文章


    authors: Sharma NK,Sharma K,Gupta A,Prabhakar S,Singh R,Gupta PK,Anand A

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    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Ojha S,Javed H,Azimullah S,Haque ME

    更新日期:2016-07-01 00:00:00

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    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Du HF,Ou LP,Song XD,Fan YR,Yang X,Tan B,Quan Z,Luo CL,Wu XH

    更新日期:2014-04-01 00:00:00