Insulin receptor binding from mid-term and full-term placentas of patients with gestational diabetes mellitus and normal pregnant women.


:Insulin receptor binding was examined in the microvillous membranes of mid-term (20-22 weeks of gestation, MT) and full-term (FT) placentas from patients with gestational diabetes mellitus (GDM) and in normal pregnant control (N). Mid-term placentas were obtained from patients who have had spontaneous abortion. The maximum per cent specific binding (%SB) in MT placenta for GDM was significantly lower (4.8%) compared with the FT placenta (22%, p < 0.001), while in the N group the maximum per cent specific binding for MT placenta was 14.1% compared with 26% for the FT placenta (p < 0.001). Binding data from FT placenta of well-controlled GDM patients were similar with the FT placenta from N group (22% SB for GDM VS 26% SB for N). Even as there were similarities in the binding characteristics of FT placentas from both groups the placental membrane protein content in the GDM group was lower by 50% compared with the N control (2.5 +/- 0.11 VS 4.8 +/- 0.15 mg protein/g placenta respectively, p < 0.001) suggesting that in the GDM group achieving a tight glycemic control could improve receptor affinities. Data from the competitive binding assay of GDM patients showed that the insulin necessary to achieve 50% inhibition (ID50) was significantly lower in MT compared with the FT placenta (0.9 x 10(-9) M VS 3.8 x 10(-9) M, p < 0.001) but in the N placenta there was no alteration in the ID50 of MT and FT placentas (3.1 x 10(-9) M VS 4 x 10(-9) M, p < 0.01, respectively). The present study demonstrated that in GDM the placental insulin receptor binding was significantly lower in spontaneously aborted placenta compared with placentas collected at full-term. Furthermore, these data suggest that the objective to achieve a tight glycemic control in GDM patients could optimize insulin receptor function similar to that of a normal pregnancy. Thus a full term placenta from GDM patients under a well managed glycemic control throughout the entire duration of pregnancy would result in an optimum insulin receptor function.


Mol Cell Biochem


al-Attas OS




Has Abstract


1995-10-04 00:00:00












  • Phe27Cys polymorphism of the human delta opioid receptor predisposes cells to compromised calcium signaling.

    abstract::A quarter of the human population with European background carries at least one allele of the OPRD1 gene that encodes the delta opioid receptor with cysteine at the amino acid position 27 (hδOR(Cys27)) instead of the evolutionary conserved phenylalanine (hδOR(Phe27)). The two variants have indistinguishable pharmacolo...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Tuusa JT,Petäjä-Repo UE

    更新日期:2011-05-01 00:00:00

  • Pharmacological inhibition of GSK-3β produces late phase of cardioprotection in hyperlipidemic rat: possible involvement of HSP 72.

    abstract::The acute, as well as late, phase of cardioprotection induced by ischemic preconditioning is abolished in hyperlipidemic (HL) rat heart. The pharmacological inhibition of glycogen synthase kinase-3β (GSK-3β), has earlier been reported to restore this attenuated acute cardioprotective effect. However, it not known whet...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Yadav HN,Singh M,Sharma PL

    更新日期:2012-10-01 00:00:00

  • HIF-1alpha involvement in low temperature and anoxia survival by a freeze tolerant insect.

    abstract::Winter survival for many insect species relies on the ability to endure the freezing of extracellular body fluids. Because freezing impedes oxygen delivery to tissues, one component of natural freeze tolerance is a well-developed anoxia/ischemia resistance. The present study explores the responses of the hypoxia-induc...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Morin P Jr,McMullen DC,Storey KB

    更新日期:2005-12-01 00:00:00

  • Na+ -transport modulation induces isoform-specific expression of Na+,K+ -Atpase alpha-subunit isoforms in C2C12 skeletal muscle cell.

    abstract::Changes in demands for Na+ transport alter expression of the Na+,K+ -ATPase subunit isoforms. In skeletal muscle, the effects of these changes on expression the alpha2 isoform, the major isoform expressed in differentiated muscle cell, is not known. Therefore, this study examines regulation of the alpha-subunit isofor...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Ladka R,Ng YC

    更新日期:2000-08-01 00:00:00

  • Direct localisation of molecules in tissue sections of growing antler tips using MALDI imaging.

    abstract::The astonishing growth rate of deer antlers offers a valuable model for the discovery of novel factors and regulatory systems controlling rapid tissue growth. Numerous molecules have been identified in growing antlers using a variety of techniques. However, little is known about the spatial distribution of these molec...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Deb-Choudhury S,Wang W,Clerens S,McMahon C,Dyer JM,Li C

    更新日期:2015-11-01 00:00:00

  • The activities of antioxidant enzymes and the level of malondialdehyde in cerebellum of rats subjected to methotrexate: protective effect of caffeic acid phenethyl ester.

    abstract::Methotrexate (MTX), a folic acid antagonist, is widely used as a cytotoxic chemotherapeutic agent. MTX-associated neurotoxicity is an important clinical problem. The aim of this study was to investigate the role of caffeic acid phenethyl ester (CAPE) on cerebellar oxidative stress induced by MTX in rats. A total of 19...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Uzar E,Koyuncuoglu HR,Uz E,Yilmaz HR,Kutluhan S,Kilbas S,Gultekin F

    更新日期:2006-10-01 00:00:00

  • NOX1 abet mesangial fibrogenesis via iNOS induction in diabetes.

    abstract::Both NADPH oxidase (NOX) and inducible nitric oxide synthase (iNOS) are the main sources of reactive oxygen species in kidney. However, their interactions in oxidative stress and contributions to kidney fibrosis during diabetic nephropathy have not been studied. Human mesangial cells were treated with normal glucose (...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Gao L,Huang W,Li J

    更新日期:2013-10-01 00:00:00

  • NSAIDs may regulate EGR-1-mediated induction of reactive oxygen species and non-steroidal anti-inflammatory drug-induced gene (NAG)-1 to initiate intrinsic pathway of apoptosis for the chemoprevention of colorectal cancer.

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    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Vaish V,Piplani H,Rana C,Vaiphei K,Sanyal SN

    更新日期:2013-06-01 00:00:00

  • Acute and chronic administration of disodium disuccinate astaxanthin (Cardax) produces marked cardioprotection in dog hearts.

    abstract::Previous results from our laboratory have shown that a novel carotenoid derivative (disodium disuccinate astaxanthin; Cardax) produced dose-related reductions in myocardial infarct size (IS) in Sprague-Dawley rats when it was administered at any of three doses (25, 50 and 75 mg/kg, iv) on four consecutive days, follow...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Gross GJ,Lockwood SF

    更新日期:2005-04-01 00:00:00

  • Adaptive character of liver glucokinase.

    abstract::1. Glucokinase is one of four glucose phosphorylating enzymes present in rat liver. Its distinctive features are a high K-m for glucose (high-K-m isozyme) and a rather narrow substrate specificity. In contrast, the other three enzymes, collectively called hexokinases or low-K-m isozymes, exhibit low K-m values for glu...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章,评审


    authors: Niemeyer H,Ureta T,Clark-Turri L

    更新日期:1975-02-28 00:00:00

  • Regulation of phospholamban and troponin-I phosphorylation in the intact rat cardiomyocytes by adrenergic and cholinergic stimuli: roles of cyclic nucleotides, calcium, protein kinases and phosphatases and depolarization.

    abstract::Protein phosphorylation was investigated in [32P]-labeled cardiomyocytes isolated from adult rat heart ventricles. The beta-adrenergic stimulation (by isoproterenol, ISO) increased the phosphorylation of inhibitory subunit of troponin (TN-I), C-protein and phospholamban (PLN). Such stimulation was largely mediated by ...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章,评审


    authors: Sulakhe PV,Vo XT

    更新日期:1995-08-01 00:00:00

  • Insulin-lecithin interaction in non-aqueous solvents and its change after application of a static electric field.

    abstract::The interaction between dipalmitoyl lecithin and egg lecithin with insulin was studied in a non-aqueous solvent such as dioxane-chloroform (1:1) by dielectric constant measurements and absorption spectra. The electrostatic character of the interaction results from the dielectric measurements and the effect of an exter...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Galzigna L,Garbin L,Rigoni F,Siliprandi N

    更新日期:1980-03-20 00:00:00

  • 3T3-L1 preadipocyte differentiation and poly(ADP-ribose) synthetase.

    abstract::Differentiation of 3T3-L1 preadipocytes, induced by methyl-isobutylxanthine (MIX), dexamethasone (DEX), and insulin, results in cells with the morphological and biochemical characteristics of adipocytes. Following incubation of 3T3-L1 cells with MIX, DEX, and insulin, poly(ADP-ribose) synthetase activity decreased abr...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章,评审


    authors: Pekala PH,Moss J

    更新日期:1983-01-01 00:00:00

  • Characterization of cholinesterase molecular forms in the mucosal cells along the intestine of the chicken.

    abstract::The presence of a butyrylcholinesterase (BuChE, EC in the musocal cells of the chicken intestine was demonstrated by histochemical and biochemical methods. The study of its distribution, along the intestine from duodenum to rectum, showed that the jejuno-ileum possesses the highest activity. Sucrose gradient ...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Sine JP,Ferrand R,Colas B

    更新日期:1989-01-23 00:00:00

  • PTPN12 controls PTEN and the AKT signalling to FAK and HER2 in migrating ovarian cancer cells.

    abstract::Several tyrosine phosphatases control cell motility; understanding their signaling helps to decipher cancer mechanisms. Previously, we found that the negative regulation of migration exerted by PTPN12 in ovarian cancer SKOV-3 cells involves direct FAK Y397 targeting, in HER2-dependent way. In this study, we describe t...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Villa-Moruzzi E

    更新日期:2013-03-01 00:00:00

  • Lupeol and its ester inhibit alteration of myocardial permeability in cyclophosphamide administered rats.

    abstract::Cyclophosphamide (CP), an alkylating agent widely used in cancer chemotherapy causes cardiac membrane damage. Lupeol, a pentacyclic triterpene, isolated from Crataeva nurvala stem bark and its ester, lupeol linoleate possess a wide range of medicinal properties. The effect of lupeol and its ester was evaluated in CP i...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Sudharsan PT,Mythili Y,Selvakumar E,Varalakshmi P

    更新日期:2006-11-01 00:00:00

  • Role of reactive oxygen species in cardiovascular aging.

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    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章,评审


    authors: Muscari C,Giaccari A,Giordano E,Clô C,Guarnieri C,Caldarera CM

    更新日期:1996-07-01 00:00:00

  • Atrial natriuretic peptide inhibits the phosphoinositide hydrolysis in murine Leydig tumor cells.

    abstract::The ability of ANP to inhibit the hydrolysis of phosphoinositides was examined in [3H] myoinositol-labeled intact murine Leydig tumor (MA-10) cells. Arginine vasopressin (AVP) stimulated the formation of inositol monophosphate (IP1), inositol bisphosphate (IP2), and inositol trisphosphate (IP3) both in a time-and dose...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Khurana ML,Pandey KN

    更新日期:1996-05-24 00:00:00

  • Mechanisms of transcriptional activation of cAMP-responsive element-binding protein CREB.

    abstract::The CREB-CREM transcription factors are the main gene regulatory effectors of the cAMP signaling pathway. The investigations of this family of transcription factors had a profound impact on the understanding of signaling-induced gene transcription. Here we discuss some key aspects of the underlying biology, review tra...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章,评审


    authors: Haus-Seuffert P,Meisterernst M

    更新日期:2000-09-01 00:00:00

  • Chronic alcohol-induced oxidative endothelial injury relates to angiotensin II levels in the rat.

    abstract::The link between chronic alcohol consumption and cardiovascular injury including hypertension is well known. However, molecular mediators implicated with alcohol-induced elevation in blood pressure (BP) remain elusive. The aim of this study was to investigate the relationship of chronic ethanol-induced endothelial inj...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Husain K,Vazquez M,Ansari RA,Malafa MP,Lalla J

    更新日期:2008-01-01 00:00:00

  • Expression of hepatic calcium-binding protein regucalcin mRNA is elevated by refeeding of fasted rats: involvement of glucose, insulin and calcium as stimulating factors.

    abstract::The effect of refeeding on the expression of Ca(2+)-binding protein regucalcin mRNA in the liver of fasted rats was investigated. When rats were fasted overnight, the hepatic regucalcin mRNA level was reduced about 70% of that in feeding rats. Refeeding produced a remarkable elevation of hepatic regucalcin mRNA level ...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Yamaguchi M,Oishi K,Isogai M

    更新日期:1995-01-12 00:00:00

  • Depressed sliding velocity of isolated cardiac myosin from cardiomyopathic hamsters: evidence for an alteration in mechanical interaction of actomyosin.

    abstract::We measured the relative sliding velocity of cardiomyopathic hamster cardiac myosin on actin cables by using an in vitro motility assay system. We also investigated the relationship between the velocity and both myosin isozyme content and ATPase activity. Cardiac myosin was obtained from cardiomyopathic hamsters (BIO ...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Yamashita H,Sugiura S,Sata M,Serizawa T,Iizuka M,Shimmen T,Momomura S

    更新日期:1993-02-17 00:00:00

  • Initiation and regulation mechanisms of ribosomal RNA transcription in the eukaryote Acanthamoeba castellanii.

    abstract::Acanthamoeba rRNA transcription involves the binding of a transcription initiation factor (TIF) to the core promoter of rDNA to form the preinitiation complex. This complex is formed in the absence of RNA polymerase I, and persists for multiple rounds of initiation. Polymerase I next binds to form the initiation compl...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章,评审


    authors: Paule MR,Bateman E,Hoffman L,Iida C,Imboden M,Kubaska W,Kownin P,Li H,Lofquist A,Risi P

    更新日期:1991-05-29 00:00:00

  • Phosphorylation of translation factors in response to anoxia in turtles, Trachemys scripta elegans: role of the AMP-activated protein kinase and target of rapamycin signalling pathways.

    abstract::Long-term survival of oxygen deprivation by animals with well-developed anoxia tolerance depends on multiple biochemical adaptations including strong metabolic rate depression. We investigated whether the AMP-activated protein kinase (AMPK) could play a regulatory role in the suppression of protein synthesis that occu...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Rider MH,Hussain N,Dilworth SM,Storey KB

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    abstract::Glucose-6-phosphatase activity was measured in rat liver or pancreatic islet crude homogenates and microsomes. The data recorded in the liver were comparable to those reported in prior studies. However, in the islets, the hydrolysis of D-glucose 6-phosphate by disrupted microsomes represented, when expressed relative ...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Perales MA,Sener A,Malaisse WJ

    更新日期:1991-02-27 00:00:00

  • Fatty acid-binding protein and its relation to fatty acid oxidation.

    abstract::A relation between fatty acid oxidation capacity and cytosolic FABP content was found in heart and various muscles of the rat. Other tissues do not show such a relation, since they are involved in more or other pathways of fatty acid metabolism. At postnatal development FABP content and fatty acid oxidation capacity r...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Veerkamp JH,van Moerkerk HT

    更新日期:1993-06-09 00:00:00

  • MiR-96 downregulates RECK to promote growth and motility of non-small cell lung cancer cells.

    abstract::MicroRNAs play critical roles in the development and progression of non-small cell lung cancer (NSCLC). miR-96 acts as an oncogene in some malignancies, while its role in NSCLC is unclear. Here, we validated that miR-96 was significantly increased in both human NSCLC tissues and cell lines. Inhibition of miR-96 expres...

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    pub_type: 杂志文章


    authors: Guo H,Li Q,Li W,Zheng T,Zhao S,Liu Z

    更新日期:2014-05-01 00:00:00

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    abstract::Adipose tissue is a major reservoir of cholesterol and, as such, it may play a significant role in cholesterol homeostasis. The aims of this study were to obtain a quantitative characterization of apolipoprotein A-I (apoA-I)-dependent lipid efflux from adipocytes and examine the role of ATP-binding cassette transporte...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Howard AD,Verghese PB,Arrese EL,Soulages JL

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  • Serum concentrations of MCP-1 and IL-6 in combination predict the presence of coronary artery disease and mortality in subjects undergoing coronary angiography.

    abstract::Cytokines play a key role in the pathogenesis of coronary artery disease (CAD). The aim of current study was to investigate the relationship between the serum concentrations of 12 cytokines with mortality and extent of CAD in individuals undergoing angiography and healthy controls. 342 CAD patients were recruited and ...

    journal_title:Molecular and cellular biochemistry

    pub_type: 临床试验,杂志文章


    authors: Tajfard M,Latiff LA,Rahimi HR,Moohebati M,Hasanzadeh M,Emrani AS,Esmaeily H,Taghipour A,Mirhafez SR,Ferns GA,Mardan-Nik M,Mohammadzadeh E,Avan A,Hanachi P,Ghayour-Mobarhan M

    更新日期:2017-11-01 00:00:00

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    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Shen HL,Xu ZG,Huang LY,Liu D,Lin DH,Cao JB,Zhang X,Wang ZQ,Wang WH,Yang PY,Han ZG

    更新日期:2009-01-01 00:00:00