Abstract:
:Fat cells were preincubated for 2 h in the presence and absence of growth hormone (GH) and Dexamethasone (Dex) before the addition of increasing concentrations of either epinephrine, theophylline or glucagon and final incubation of the cells for an additional 5 minutes. GH and Dex increased by 85%, 28% and 72%, respectively, the cAMP levels reached in the sole presence of 10(-5)M epinephrine, 10(-2)M theophylline or 5 X 10(-5)M glucagon. An adenylate cyclase particulate preparation shows that epinephrine decreases Km from 2mM to 0.6 MM and increases Vmax and the strength of interaction value (n) from 0.91 to 1.75.
journal_name
Mol Cell Biochemjournal_title
Molecular and cellular biochemistryauthors
Harris R,Bennun Adoi
10.1007/BF01731776subject
Has Abstractpub_date
1976-12-10 00:00:00pages
141-6issue
3eissn
0300-8177issn
1573-4919journal_volume
13pub_type
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