Abstract:
:Overexpressed cysteine-rich protein 61 (Cyr61) is believed to enhance osteosarcoma (OS) cell metastasis, but the mechanism of Cyr61 overexpression in OS is not clear so far. In this study 33 OS samples were analyzed by immunostaining and focused on two parts: the correlation between overexpression of Cyr61 and OS metastasis; the mechanism of regulating Cyr61 expression in OS. Twenty-five out of 33 cases (75.76 %) with metastasis showed high expression of Cyr61. Furthermore, Cyr61 expression in Saos-2 cells was reduced by siRNA, and lower expression of Cyr61 in Saos-2 cell resulted in a cell migration deficiency and had no effect on cell proliferation. Particularly, Cyr61 expression was significantly increased in Saos-2 cells in response to different dosages of transforming growth factor beta (TGF-β), indicating that the expression of Cyr61 is TGF-β dependent. A transwell assay showed that Saos-2 cells stimulated with TGF-β had a greater capacity for migration than the control cells. The p38 MAPK-specific inhibitor SB203580 was able to reduce Cyr61 expression and inhibit the migration of Saos-2 cells stimulated with TGF-β. These results obtained provide new evidence that overexpressed Cyr61 plays a key role in the metastasis of OS cells and Cyr61 is a potential target downstream of TGF-β/p38 MAPK to regulate cell migration.
journal_name
Mol Cell Biochemjournal_title
Molecular and cellular biochemistryauthors
Chen J,Song Y,Yang J,Gong L,Zhao P,Zhang Y,Su Hdoi
10.1007/s11010-013-1807-3subject
Has Abstractpub_date
2013-12-01 00:00:00pages
269-77issue
1-2eissn
0300-8177issn
1573-4919journal_volume
384pub_type
杂志文章abstract::Nocturnin has been identified as a clock-controlled gene based on its rhythmic expression and night-time peak of transcript level in Xenopus retina. Further studies show that the widespread expression and rhythmicity of nocturnin mRNA level parallel the expression of clock genes. In Xenopus, nocturnin transcription is...
journal_title:Molecular and cellular biochemistry
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journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章,评审
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journal_title:Molecular and cellular biochemistry
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journal_title:Molecular and cellular biochemistry
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journal_title:Molecular and cellular biochemistry
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doi:10.1007/BF01731729
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journal_title:Molecular and cellular biochemistry
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abstract::The effects of different dietary fats on peritoneal macrophage plasma membrane fluidity, intracellular cyclic AMP (cAMP) production, GTP hydrolysis and TNF binding and TNF-induced IL1 and IL6 production was investigated. After a four week period, fluidity, as determined by both fluorescence recovery after photobleachi...
journal_title:Molecular and cellular biochemistry
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doi:10.1023/a:1006875010120
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journal_title:Molecular and cellular biochemistry
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journal_title:Molecular and cellular biochemistry
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abstract::Gastric cancer (GC) is a malignant tumor with high morbidity and mortality in the world. Circular RNA hsa_circHN1_005 (circ_HN1), also termed as hsa_circ_0045602, is reported as an oncogene in GC. However, the molecular mechanism of circ_HN1 in GC development has not been fully explored. Here, we surveyed the regulato...
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