Abstract:
:Murine erythroleukemia cell lines derived from Friend virus infected mice can be induced to differentiate in vitro by numerous agents. Among these compounds are certain naturally occurring purines such as hypoxanthine or 1-methylhypoxanthine. We have extended these studies to other modified oxypurines and have identified some areas of cell regulation with which they may be interacting. Monomethylated derivatives of guanine, hypoxanthine or xanthine are active as inducers of differentiation. Excluding hypoxanthine, the parent oxypurines guanine and xanthine are ineffective in inducing differentiation. The dimethyl- and trimethylxanthine derivatives are also inactive as inducers. The methylated oxypurines are not metabolized to nucleotides by the cell and, therefore, probably do not interact with nucleic acid synthesis directly. We have investigated one cellular process of possible regulatory significance with which they do interact. ADP-ribosylation has been implicated in control of gene expression and differentiation. The methylated oxypurines inhibit this reaction, as measured in permeabilized cells, in the same concentration range at which they are effective as inducers of differentiation. Additionally, 1-methylguanine and 7-methylguanine decrease incorporation of mannose and glucosamine into glycoprotein and into dolichol-oligosaccharide precursors. These effects may be related to cell surface alterations observed during differentiation.
journal_name
Mol Cell Biochemjournal_title
Molecular and cellular biochemistryauthors
Kerr SJdoi
10.1007/BF00220717subject
Has Abstractpub_date
1990-01-18 00:00:00pages
37-44issue
1eissn
0300-8177issn
1573-4919journal_volume
92pub_type
杂志文章abstract::Adipocytes from 26-week-old Zucker rats (greater than 600 g) and controls of the same strain and age (greater than 400 g) served as models of insulin resistance and obesity. Adipocytes from fatty rats demonstrated insulin resistance of glucose metabolism and resistance to the antilipolytic effect of insulin which was ...
journal_title:Molecular and cellular biochemistry
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journal_title:Molecular and cellular biochemistry
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journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
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journal_title:Molecular and cellular biochemistry
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journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/BF00926857
更新日期:1993-02-17 00:00:00