Semiquantitative RT-PCR method coupled to capillary electrophoresis to study 5alpha-reductase mRNA isozymes in rat ventral prostate in different androgen status.

abstract：:Fructose consumption is responsible for the onset of insulin resistance (IR), and metabolic syndrome. It possesses no functional utility in body and its detrimental effects on hepatic metabolic milieu are beyond those produced by glucose. The need of the hour is to identify fructose-induced IR as an unique pathologica...

journal_title：Molecular and cellular biochemistry

authors： Aggarwal H,Nair J,Sharma P,Sehgal R,Naeem U,Rajora P,Mathur R

更新日期：2018-01-01 00:00:00

abstract：:Winter survival for many insect species relies on the ability to endure the freezing of extracellular body fluids. Because freezing impedes oxygen delivery to tissues, one component of natural freeze tolerance is a well-developed anoxia/ischemia resistance. The present study explores the responses of the hypoxia-induc...

journal_title：Molecular and cellular biochemistry

authors： Morin P Jr,McMullen DC,Storey KB

更新日期：2005-12-01 00:00:00

abstract：:This study focuses on two inflammatory diseases, viz., "diabetes mellitus (DM)" that causes serious complications such as retinopathy, nephropathy, and neuropathy, and "ischemic colitis" which is evoked by DM. Ischemic colitis originates from the reduction in mesenteric blood flow to the colon with existence of the oc...

journal_title：Molecular and cellular biochemistry

authors： Ozel Y,Elcioglu HK,Cevikelli ZA,Kudas I,Ahmad S,Uzun H,Topal C,Aktas S,Kabasakal L

更新日期：2018-02-01 00:00:00

abstract：:Recruitment of the protein kinase C (PKC) family of isozymes is an integral component of the signaling events that direct cardiac phenotype expressed during postnatal development and in response to pathologic stimuli. Hyperglycemia is a potent activating signal for cardiac PKC isozymes and induces the apoptosis progra...

journal_title：Molecular and cellular biochemistry

authors： Malhotra A,Kang BP,Hashmi S,Meggs LG

更新日期：2005-01-01 00:00:00

abstract：:Targeting angiogenesis is considered an effective strategy for treating the expansion and metastasis of tumors. The aim of this study is to assess the effects of perifosine, an inhibitor of Akt, on cell proliferation, apoptosis, angiogenesis, and VEGF-induced cell migration in cultured human umbilical vein endothelial...

journal_title：Molecular and cellular biochemistry

authors： Wang FZ,Fei HR,Li XQ,Shi R,Wang de C

更新日期：2012-09-01 00:00:00

abstract：:Hepatocellular carcinoma (HCC) is one of leading causes of cancer-related death, and its increasing incidence worldwide is a cause for concern. The recombinant analgesic-antitumor peptide (rAGAP), a protein consisting of small ubiquitin-related modifier linked with a hexa-histidine tag, exhibited the antitumor activit...

journal_title：Molecular and cellular biochemistry

authors： Cao Q,Lu W,Zhou T,Liu Y,Cai X,Zhu J,Cao P

更新日期：2019-05-01 00:00:00

abstract：:To identify and characterize endothelial cell surface components that bind plasminogen, we used ligand-blotting to study binding of plasminogen to sodium dodecyl sulphate solubilized extracts of human umbilical vein endothelial cells. It was observed that glu-plasminogen bound predominantly to a 45 kDa endothelial cel...

journal_title：Molecular and cellular biochemistry

authors： Dudani AK,Hashemi S,Aye MT,Ganz PR

更新日期：1991-12-11 00:00:00

abstract：:The native form of phospholamban is not known and it is presently under debate whether this protein exists as a monomer or an oligomer in cardiac sarcoplasmic reticulum. The currently accepted model for phospholamban is pentameric, based primarily on its behavior in SDS-polyacrylamide gel electrophoresis. In this stud...

journal_title：Molecular and cellular biochemistry

authors： Harrer JM,Kranias EG

更新日期：1994-11-23 00:00:00

abstract：:CDK11(p58), a G2/M-specific protein kinase, has been shown to be associated with apoptosis in many cell lines, with largely unknown mechanisms. Our previous study proved that CDK11(p58)-enhanced cycloheximide (CHX)-induced apoptosis in SMMC-7721 hepatocarcinoma cells. Here we report for the first time that ectopic exp...

journal_title：Molecular and cellular biochemistry

authors： Yun X,Wu Y,Yao L,Zong H,Hong Y,Jiang J,Yang J,Zhang Z,Gu J

更新日期：2007-10-01 00:00:00

abstract：:We previously reported that curcumin induces browning of primary white adipocytes via enhanced expression of brown adipocyte-specific genes. In this study, we attempted to identify target proteins responsible for this fat-browning effect by analyzing proteomic changes in cultured white adipocytes in response to curcum...

journal_title：Molecular and cellular biochemistry

authors： Kim SW,Choi JH,Mukherjee R,Hwang KC,Yun JW

更新日期：2016-04-01 00:00:00

abstract：:Rat Langendorff hearts perfused with media that do not contain erythrocytes or fluorocarbon as oxygen carriers are borderline aerobic during 5 Hz pacing. This follows from the release of catabolic products measured: lactate, urate and Iysophosphatidyl-choline (IysoPC). Addition of L-carnitine to the perfusion medium r...

journal_title：Molecular and cellular biochemistry

authors： Hulsmann WC,Peschechera A,Serafini F,Ferrari LE

更新日期：1996-03-09 00:00:00

abstract：:Failure in obtaining expression of functional adrenocorticotropic hormone receptor (ACTHR, or melanocortin 2 receptor, MC2R) in non-adrenal cells has hindered molecular analysis of ACTH signaling pathways. Here, we ectopically expressed the mouse ACTHR in Balb/c mouse 3T3 fibroblasts to analyze ACTH signaling pathways...

journal_title：Molecular and cellular biochemistry

authors： Forti FL,Dias MH,Armelin HA

更新日期：2006-12-01 00:00:00

abstract：:Formation of foam cells from macrophages, which are formed by the differentiation of blood-borne monocytes, is a critical early event in atherogenesis. To examine how pre-exposure of monocytes to modified proteins, such as oxLDL, influences their differentiation to macrophages, an in vitro model system using isolated ...

journal_title：Molecular and cellular biochemistry

authors： Radhika A,Sudhakaran PR

更新日期：2013-01-01 00:00:00

abstract：:MicroRNAs (miRNAs, miRs) play important roles in pathogenesis and development of human diseases, including malignancy. Some may affect tumor progression through targeting tumor suppressor genes. MiR-135b has been shown to be upregulated in CRC. In this study, we evaluated the role of miR-135b in colorectal cancer (CRC...

journal_title：Molecular and cellular biochemistry

authors： Wu W,Wang Z,Yang P,Yang J,Liang J,Chen Y,Wang H,Wei G,Ye S,Zhou Y

更新日期：2014-03-01 00:00:00

abstract：:Diabetes-induced changes in glucose formation, intracellular and mitochondrial glutathione redox states as well as hydroxyl free radicals (HFR) generation have been investigated in rabbit kidney-cortex tubules. In contrast to renal tubules of control animals, diabetes-evoked increase in glucose formation in the presen...

journal_title：Molecular and cellular biochemistry

authors： Winiarska K,Drozak J,Wegrzynowicz M,Fraczyk T,Bryla J

更新日期：2004-06-01 00:00:00

abstract：:Oxygen is a diradical and because of its unique electronic configuration, it has the potential to form strong oxidants (e.g. superoxide radical, hydrogen peroxide and hydroxyl radical) called oxygen free radicals or partially reduced forms of oxygen (PRFO). These highly reactive oxygen species can cause cellular injur...

journal_title：Molecular and cellular biochemistry

authors： Singal PK,Dhalla AK,Hill M,Thomas TP

更新日期：1993-12-22 00:00:00

abstract：:Transport characteristics of essential trace elements as zinc, copper, selenium and iron have been studied in maternal-fetal direction in normal pregnancies, using in vitro perfusion of human placental lobules. Solutions of trace elements corresponding to twice the physiological concentrations were injected (100 micro...

journal_title：Molecular and cellular biochemistry

authors： Nandakumaran M,Dashti HM,Al-Saleh E,Al-Zaid NS

更新日期：2003-10-01 00:00:00

abstract：:This comparative study investigates the relationship between sarcoplasmic reticulum (SR) calcium(Ca2+)-ATPase transport activity and phospholamban (PLB) phosphorylation in whole cardiac homogenates of spontaneously hypertensive rats (SHR) and their parent, normotensive Wistar Kyoto (WKY) strain during early postnatal ...

journal_title：Molecular and cellular biochemistry

authors： Freestone N,Singh J,Krause EG,Vetter R

更新日期：1996-10-01 00:00:00

abstract：:In this study we assessed activities of antioxidant enzymes, lipid peroxidation end-products, and nitric oxide (NO) levels in women with postmenopausal osteoporosis (PMO). Relationship between oxidative stress parameters and NO levels with bone mineral density (BMD) and clinical variables influencing bone mass and hea...

journal_title：Molecular and cellular biochemistry

authors： Ozgocmen S,Kaya H,Fadillioglu E,Aydogan R,Yilmaz Z

更新日期：2007-01-01 00:00:00

abstract：:The effect of peritoneal dialysis (PD) with high-glucose dialysis fluid on the VEGF family, tristetraprolin (TTP), angiogenesis and lymphangiogenesis was investigated. Forty male SD rats were randomised into five groups: normal group, sham operation group, uraemia group, PD 2-week group and PD4-week group. After 4 wee...

journal_title：Molecular and cellular biochemistry

authors： Xiao J,Gao H,Jin Y,Zhao Z,Guo J,Liu Z,Zhao Z

更新日期：2014-07-01 00:00:00

abstract：:Cultured cardiac myocytes were depleted of ATP by incubation with oligomycin (1 mg/ml). Then the ability of the cells to oxidize various substrates and to restore ATP levels was studied. Following ATP depletion, the cells were found to be able to oxidize glucose given alone, but not palmitate. However, with both subst...

journal_title：Molecular and cellular biochemistry

authors： Tirosh R,Mishor T,Pinson A

更新日期：1996-09-20 00:00:00

abstract：:Cutaneous changes like rash and hair loss, as well as other neurogenic inflammation side effects, occur frequently during anticancer treatment with the epidermal growth factor receptor-tyrosine kinase inhibitor (EGFR-TKI), erlotinib. These adverse events may be so severe that they impair the patient's compliance with ...

journal_title：Molecular and cellular biochemistry

authors： Chmielinska JJ,Kramer JH,Mak IT,Spurney CF,Weglicki WB

更新日期：2020-02-01 00:00:00

abstract：:The homeodomain-containing Msx3 gene, the newest member of the Msx family, encodes two mRNAs, with an unknown relationship to each other. To elucidate how Msx3 gene generates the two transcripts, we cloned their corresponding cDNAs from an E10.5 mouse embryo cDNA library. The alignment of sequences of the two Msx3-spe...

journal_title：Molecular and cellular biochemistry

authors： Matsui H,Takahashi T,Raghow R

更新日期：2004-12-01 00:00:00

abstract：:Signal transduction pathways control various biological processes in cells leading to distinct cellular functions. Protein-protein interactions and post-translational modifications are the physiological events that occur in signaling pathway. p38 MAPK are known to be involved in regulating wide range of cellular proce...

journal_title：Molecular and cellular biochemistry

authors： Rini J,Anbalagan M

更新日期：2017-11-01 00:00:00

abstract：:Three antisera were prepared against non-histone protein classes named NHCP1, NHCP2 and dehistonized chromatin (with different affinity to DNA) from hamster liver. Two main antigenic bands of MW 17,000 and 36,000 were specific in the NHCP1 fraction and one antigen of MW 56,000 was specific for the NHCP2 fraction from ...

journal_title：Molecular and cellular biochemistry

authors： Wojtkowiak Z,Kiliańska Z,Kłyszejko-Stefanowicz L

更新日期：1990-01-18 00:00:00

abstract：:The influence of hypotonic solution on cell volume and electrophysiology properties of the failing heart of cardiomyopathic hamsters (TO-2) was investigated. The results showed an increase in cell volume of quiescent isolated ventricular myocytes by 66% within 30 min. Angiotensin (1-7) [Ang (1-7)] (10(-8) M) administe...

journal_title：Molecular and cellular biochemistry

authors： De Mello WC

更新日期：2009-10-01 00:00:00

abstract：:Prolonged ischemia-reperfusion results in various damages in skeletal muscle. Following reperfusion, although the damaged muscles undergo regeneration, the precise process and mechanism of regeneration have not yet been fully understood. Here, we show the altered levels of plasma biochemical markers of muscle damage, ...

journal_title：Molecular and cellular biochemistry

authors： Itoh M,Shimokawa N,Tajika Y,Murakami T,Aotsuka N,Lesmana R,Farenia R,Iwasaki T,Okda J,Yorifuji H,Koibuchi N

更新日期：2013-01-01 00:00:00

abstract：:Those genes which are transcribed by RNA polymerase III continue to give surprising results with respect to their cis-acting elements and transacting factors. As a result, a broader view of class III promoters has emerged and the internal promoters are not universal in classical polymerase III genes. The involvement o...

journal_title：Molecular and cellular biochemistry

authors： Shastry BS

更新日期：1993-07-07 00:00:00

abstract：:The present review is focused on the main achievements realized in the lysozyme research field since the meeting held in 1972 to commemorate the fiftieth anniversary of the discovery of this enzyme. Despite of extensive structural, physico-chemical, crystallographic, genetic, immunological and evolutionary studies dev...

journal_title：Molecular and cellular biochemistry

authors： Jollès P,Jollès J

更新日期：1984-09-01 00:00:00

abstract：:Administration of Ca2+ channel blockers in cardiac disorders and the central role of Ca2+ in modulating neutrophil functions, prompted us to investigate whether administration of nifedipine to mice would alter their natural resistance to infectious agents like Salmonella typhimurium. Neutrophil chemiluminescence (CL) ...

journal_title：Molecular and cellular biochemistry

authors： Nalini K,Andrabi KI,Ganguly NK,Wahi PL

更新日期：1990-06-25 00:00:00