Alteration of antioxidant status following sympathectomy: differential effects of modified plasma levels of adrenaline and noradrenaline.


:The influence of altered levels of endogenous catecholamines following adrenalectomy or 6-hydroxydopamine (6-OH) treatment (alone or in combination) on enzymatic (glutathione reductase, catalase, glutathione peroxidase and Cu, Zn superoxide dismutase) and non-enzymatic (glutathione) antioxidant components of heart, liver, kidney, lung and erythrocytes in male Wistar rats was investigated. Functional antioxidant status was assessed in terms of susceptibility to t-butylhydroperoxide-induced sulfhydryl group oxidation (an indirect measure of glutathione depletion) and lipid peroxidation, as measured by thiobarbituric acid-reactive substance (TBARS) formation. Reduced levels of adrenaline and noradrenaline resulted from adrenalectomy and 6-OH treatment, respectively, while a combination of these treatments led to a reduction in the levels of both catecholamines. Adrenalectomy was associated with alterations in glutathione reductase activity in the heart and liver (increased). 6-OH treatment alone produced an elevation in glutathione reductase activity only in the heart. In adrenalectomized animals, 6-OH treatment produced no further increases in glutathione reductase activities of heart or liver. In lung, however, the combination of adrenalectomy and 6-OH treatment caused an elevation in both glutathione peroxidase and glutathione reductase activities. Glutathione levels of liver alone were elevated following adrenalectomy, while those of erythrocytes and liver (but not other tissues investigated) were increased by the combination of adrenalectomy and 6-OH treatment. The kidney was relatively resistant to the effects of sympathectomy and showed no changes in any of the antioxidant components measured. Adrenalectomy alone or in combination with 6-OH produced an increased in susceptibility to peroxide-induced sulfhydryl group oxidation only in the heart. 6-OH treatment caused a reduction in peroxide-induced TBARS formation only in the kidney. Both adrenalectomy and the combination of adrenalectomy and 6-OH treatment were associated with reduced TBARS formation in the liver, lung and kidney, but not heart. Results from this study demonstrate that the effects of sympathectomy on antioxidant status vary among tissues. Differences between adrenalectomy and 6-OH treatment on antioxidant components are suggestive of differential actions of adrenaline and noradrenaline on tissue antioxidant status which may have important implications under conditions associated with elevations in levels of these catecholamines including chronic stress and myocardial infarction.


Mol Cell Biochem


Toleikis PM,Godin DV


Has Abstract


1995-11-08 00:00:00












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    abstract::The present paper shows that human chronic myeloid (K562) cells exposed 3 h to 20 microM 3'-azido-3'-deoxythymidine (AZT) exhibit marked variations of the oligosaccharide moiety of glycoconjugates. These changes were analyzed by confocal fluorescence microscopy, upon incubation of control and AZT-treated cells with bi...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Lizzi AR,D'Alessandro AM,Bozzi A,Cinque B,Oratore A,D'Andrea G

    更新日期:2007-06-01 00:00:00

  • Human tau protein forms complex with PrP and some GSS- and fCJD-related PrP mutants possess stronger binding activities with tau in vitro.

    abstract::Microtubule associated protein tau is considered to play roles in some types of human transmissible spongiform encephalopathies (TSE). In this study, the full-length and several truncated human tau proteins were expressed from E. coli and purified. Using GST pull down, co-immunoprecipitation assay and tau-coated ELISA...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Wang XF,Dong CF,Zhang J,Wan YZ,Li F,Huang YX,Han L,Shan B,Gao C,Han J,Dong XP

    更新日期:2008-03-01 00:00:00

  • Effect of sex and bezafibrate on incorporation of blood borne palmitate into lipids of rat liver nuclei.

    abstract::The aim of the present study was to investigate whether lipid metabolism in the nuclei is affected by changes in the metabolism of free fatty acids in the liver. The experiments were carried out on 3 groups of rats: 1 - control-male, 2 - female, and 3 - male, treated with bezafibrate (a peroxisome proliferator). The r...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Górski J,Zendzian-Piotrowska M,Wolfrum C,Nawrocki A,Spener F

    更新日期:2000-11-01 00:00:00

  • Non-invasive quantitative 31P MRS assay of mitochondrial function in skeletal muscle in situ.

    abstract::A method for non-invasive, quantitative 31P NMR spectroscopic investigation of mitochondrial function in human skeletal muscle in situ is described. High time resolution 31P NMR measurements of phosphocreatine, inorganic phosphate and ATP resonances were conducted on human forearm flexor muscle during involuntary twit...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Jeneson JA,Wiseman RW,Kushmerick MJ

    更新日期:1997-09-01 00:00:00

  • LDL oxidation by arterial wall macrophages depends on the oxidative status in the lipoprotein and in the cells: role of prooxidants vs. antioxidants.

    abstract::Oxidized LDL is highly atherogenic as it stimulates macrophage cholesterol accumulation and foam cell formation, it is cytotoxic to cells of the arterial wall and it stimulates inflammatory and thrombotic processes. LDL oxidation can lead to its subsequent aggregation, which further increases cellular cholesterol accu...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章,评审


    authors: Aviram M,Fuhrman B

    更新日期:1998-11-01 00:00:00

  • Splicing factors differentially expressed in psoriasis alter mRNA maturation of disease-associated EDA+ fibronectin.

    abstract::The EDA+ fibronectin splicing variant is overexpressed in psoriatic non-lesional epidermis and sensitizes keratinocytes to mitogenic signals. However, regulation of its abundance is only partially understood. In our recent cDNA microarray experiment, we identified three SR-rich splicing factors-splicing factor, argini...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Szlavicz E,Szabo K,Groma G,Bata-Csorgo Z,Pagani F,Kemeny L,Szell M

    更新日期:2017-12-01 00:00:00

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    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Ohya Y,Sperelakis N

    更新日期:1988-03-01 00:00:00

  • Mitochondrial genes and cell heredity.

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    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章,评审


    authors: Neifakh SA

    更新日期:1977-02-04 00:00:00

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    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Bürkle A,Müller M,Wolf I,Küpper JH

    更新日期:1994-09-01 00:00:00

  • Integration of biochemical functions of different cells of rat gastric mucosa for hydrochloric acid secretion.

    abstract::The regulation patterns of gastric acid secretion in rats were investigated. Pentagastrin and histamine stimulate gastric acid secretion, but the inhibitors of DNA-dependent synthesis of RNA and of proteins prevent only the pentagastrin action. It has been found that pentagastrin induces histidine decarboxylase in gas...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Salganik RI,Bersimbaev RI,Argutinskaya SV,Kiseleva EV,Khristolyubova NB,Deribas VI

    更新日期:1976-09-30 00:00:00

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    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Dam TK,Sarkar M,Ghosal J,Choudhury A

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  • Methylation of the promoter A of estrogen receptor alpha gene in hBMSC and osteoblasts and its correlation with homocysteine.

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    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Lv H,Ma X,Che T,Chen Y

    更新日期:2011-09-01 00:00:00

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    pub_type: 杂志文章


    authors: Durrieu G,Letellier T,Antoch J,Deshouillers JM,Malgat M,Mazat JP

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    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Derebecka-Holysz N,Lehmann TP,Holysz M,Trzeciak WH

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  • Human intestine epithelial cell acetyl- and butyrylcholinesterase.

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    pub_type: 杂志文章


    authors: Sine JP,Ferrand R,Cloarec D,Lehur PA,Colas B

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    journal_title:Molecular and cellular biochemistry

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    journal_title:Molecular and cellular biochemistry

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    authors: Srivastava RA

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    authors: Yamaoka K,Kameyama M

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    authors: Zhang J,Zhao J,Bai Y,Huang L,Yu W,Li X

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    pub_type: 杂志文章


    authors: Anand-Srivastava MB,Palaparti A,Pion J

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  • Identification of G-proteins in rat parotid gland plasma membranes and granule membranes: presence of distinct components in granule membranes.

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    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Ali N,Agrawal DK,Cheung P

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    authors: Eddy SF,Morin P Jr,Storey KB

    更新日期:2005-01-01 00:00:00

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    authors: Balakumar M,Raji L,Prabhu D,Sathishkumar C,Prabu P,Mohan V,Balasubramanyam M

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    authors: Yang B,Zhou Z,Li X,Niu J

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    authors: De Lucca FL,Sales VS,Souza LR,Murad JM,Watanabe MA

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    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Ozel Y,Elcioglu HK,Cevikelli ZA,Kudas I,Ahmad S,Uzun H,Topal C,Aktas S,Kabasakal L

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    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Tang JY,Jin P,He Q,Lu LH,Ma JP,Gao WL,Bai HP,Yang J

    更新日期:2017-01-01 00:00:00

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    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Zhao R,Ma X,Shen GX

    更新日期:2008-10-01 00:00:00

  • Expression of alternatively spliced variants of Na-Ca-exchanger-1 in experimental colitis: role in reduced colonic contractility.

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    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Shubair M,Oriowo MA,Khan I

    更新日期:2012-11-01 00:00:00