Alteration of antioxidant status following sympathectomy: differential effects of modified plasma levels of adrenaline and noradrenaline.


:The influence of altered levels of endogenous catecholamines following adrenalectomy or 6-hydroxydopamine (6-OH) treatment (alone or in combination) on enzymatic (glutathione reductase, catalase, glutathione peroxidase and Cu, Zn superoxide dismutase) and non-enzymatic (glutathione) antioxidant components of heart, liver, kidney, lung and erythrocytes in male Wistar rats was investigated. Functional antioxidant status was assessed in terms of susceptibility to t-butylhydroperoxide-induced sulfhydryl group oxidation (an indirect measure of glutathione depletion) and lipid peroxidation, as measured by thiobarbituric acid-reactive substance (TBARS) formation. Reduced levels of adrenaline and noradrenaline resulted from adrenalectomy and 6-OH treatment, respectively, while a combination of these treatments led to a reduction in the levels of both catecholamines. Adrenalectomy was associated with alterations in glutathione reductase activity in the heart and liver (increased). 6-OH treatment alone produced an elevation in glutathione reductase activity only in the heart. In adrenalectomized animals, 6-OH treatment produced no further increases in glutathione reductase activities of heart or liver. In lung, however, the combination of adrenalectomy and 6-OH treatment caused an elevation in both glutathione peroxidase and glutathione reductase activities. Glutathione levels of liver alone were elevated following adrenalectomy, while those of erythrocytes and liver (but not other tissues investigated) were increased by the combination of adrenalectomy and 6-OH treatment. The kidney was relatively resistant to the effects of sympathectomy and showed no changes in any of the antioxidant components measured. Adrenalectomy alone or in combination with 6-OH produced an increased in susceptibility to peroxide-induced sulfhydryl group oxidation only in the heart. 6-OH treatment caused a reduction in peroxide-induced TBARS formation only in the kidney. Both adrenalectomy and the combination of adrenalectomy and 6-OH treatment were associated with reduced TBARS formation in the liver, lung and kidney, but not heart. Results from this study demonstrate that the effects of sympathectomy on antioxidant status vary among tissues. Differences between adrenalectomy and 6-OH treatment on antioxidant components are suggestive of differential actions of adrenaline and noradrenaline on tissue antioxidant status which may have important implications under conditions associated with elevations in levels of these catecholamines including chronic stress and myocardial infarction.


Mol Cell Biochem


Toleikis PM,Godin DV


Has Abstract


1995-11-08 00:00:00












  • The transcription factor TBX2 regulates melanogenesis in melanocytes by repressing Oca2.

    abstract::The T-box transcription factor TBX2 is known for its role as a critical regulator of melanoma cell proliferation, but its role in regulating melanogenesis has not been widely studied. Here we use a series of experiments to show in primary and immortalized mouse melanocytes that TBX2 acts as regulator of melanogenesis ...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Chen Y,Pan L,Su Z,Wang J,Li H,Ma X,Liu Y,Lu F,Qu J,Hou L

    更新日期:2016-04-01 00:00:00

  • Cysteine-rich secretory protein 3 plays a role in prostate cancer cell invasion and affects expression of PSA and ANXA1.

    abstract::Cysteine-rich secretory protein 3 (CRISP-3) is upregulated in prostate cancer as compared to the normal prostate tissue. Higher expression of CRISP-3 has been linked to poor prognosis and hence it has been thought to act as a prognostic marker for prostate cancer. It is proposed to have a role in innate immunity but i...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Pathak BR,Breed AA,Apte S,Acharya K,Mahale SD

    更新日期:2016-01-01 00:00:00

  • COMP-Ang1 enhances DNA synthesis and cell cycle progression in human periodontal ligament cells via Tie2-mediated phosphorylation of PI3K/Akt and MAPKs.

    abstract::Recombinant COMP-Ang1, a chimera of angiopoietin-1 (Ang1), and a short coiled-coil domain of cartilage oligomeric matrix protein (COMP) can stimulate multiple cellular processes. Proliferative capacity of periodontal ligament (PDL) fibroblasts (PLFs) is important for maintaining PDL integrity and homeostasis. In this ...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Lim SS,Kook SH,Lee JC

    更新日期:2016-05-01 00:00:00

  • Control of mitochondrial respiration in muscle.

    abstract::Control of mitochondrial respiration depends on ADP availability to the F1-ATPase. An electrochemical gradient of ADP and ATP across the mitochondrial inner membrane is maintained by the adenine nucleotide translocase which provides ADP to the matrix for ATP synthesis and ATP for energy-dependent processes in the cyto...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章,评审


    authors: McMillin JB,Pauly DF

    更新日期:1988-06-01 00:00:00

  • β-Caryophyllene, a phytocannabinoid attenuates oxidative stress, neuroinflammation, glial activation, and salvages dopaminergic neurons in a rat model of Parkinson disease.

    abstract::Parkinson disease (PD) is a neurodegenerative disease characterized by progressive dopaminergic neurodegeneration in the substantia nigra pars compacta (SNc) area. The present study was undertaken to evaluate the neuroprotective effect of β-caryophyllene (BCP) against rotenone-induced oxidative stress and neuroinflamm...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Ojha S,Javed H,Azimullah S,Haque ME

    更新日期:2016-07-01 00:00:00

  • Cytokine G-protein signaling crosstalk in cardiomyocytes: attenuation of Jak-STAT activation by endothelin-1.

    abstract::The IL-6-related cytokines, LIF and cardiotrophin-1, are important growth promoting and cardioprotective agents for cardiomyocytes. However, factors that regulate their actions in the heart are poorly understood. In this study, we tested the hypothesis that endothelin-1, a peptide hormone that produces a pattern of ca...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Booz GW,Day JN,Speth R,Baker KM

    更新日期:2002-11-01 00:00:00

  • Erythrocyte-mediated microinjection of horseradish peroxidase into BHK cells. Subcellular localization by light and electron microscopy.

    abstract::In order to establish the distribution with time of proteins microinjected into mammalian cells, horseradish peroxidase (HRP) was microinjected into baby hamster kidney (BHK) cells using chicken erythrocyte ghosts. At time intervals following initiation of fusion between ghosts and target cells, samples were fixed wit...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: McClung JK,Ulrich RG,Kletzien RF

    更新日期:1987-01-01 00:00:00

  • Ischemic colitis of the colon in streptozotocin-induced diabetic rats.

    abstract::This study focuses on two inflammatory diseases, viz., "diabetes mellitus (DM)" that causes serious complications such as retinopathy, nephropathy, and neuropathy, and "ischemic colitis" which is evoked by DM. Ischemic colitis originates from the reduction in mesenteric blood flow to the colon with existence of the oc...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Ozel Y,Elcioglu HK,Cevikelli ZA,Kudas I,Ahmad S,Uzun H,Topal C,Aktas S,Kabasakal L

    更新日期:2018-02-01 00:00:00

  • Differential binding of rat pituitary-specific nuclear factors to the 5'-flanking region of pituitary and placental members of the human growth hormone gene family.

    abstract::Placental chorionic somatomammotropin (hCS-A or B) and growth hormone variant (hGH-V) are members of the human growth hormone family, and are related by structure and function to pituitary growth hormone (hGH-N). However, while the hGH-N gene is expressed specifically in the anterior pituitary, hGH-V and hCS are produ...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Nickel BE,Nachtigal MW,Bock ME,Cattini PA

    更新日期:1991-08-14 00:00:00

  • Characterization of the filamentous hemagglutinin from Bordetella pertussis by gel electrophoresis.

    abstract::A highly purified preparation of filamentous hemagglutinin (FHA) from Bordetella pertussis was analyzed for its protein composition by gel electrophoretic methods. In this preparation of FHA the following native species could be detected by polyacrylamide gel electrophoresis (PAGE) at pH 3.2: S1 and S2 (inactive subun...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: An der Lan B,Cowell JL,Burstyn DG,Manclark CR,Chrambach A

    更新日期:1986-04-01 00:00:00

  • The cell cycle regulator protein P16 and the cellular senescence of dental follicle cells.

    abstract::Cellular senescence is a restricting factor for regenerative therapies with somatic stem cells. We showed previously that the onset of cellular senescence inhibits the osteogenic differentiation in stem cells of the dental follicle (DFCs), although the mechanism remains elusive. Two different pathways are involved in ...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Morsczeck C,Hullmann M,Reck A,Reichert TE

    更新日期:2018-02-01 00:00:00

  • Vitamin A inhibits lipoperoxidation ascorbate-Fe++ dependent of rat kidney microsomes and mitochondria.

    abstract::In the present study it was investigated if Vitamin A supplementation could protect rat kidney microsomes and mitochondria from in vitro lipoperoxidation. After incubation of rat kidney microsomes and mitochondria in an ascorbate-Fe+2 system, at 37 degrees C during 60 min, it was observed that the total cpm/mg protein...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Piergiacomi VA,Palacios A,Catalá A

    更新日期:1996-12-20 00:00:00

  • A single step purification of a sialic acid binding lectin (AchatininH) from Achatina fulica snail.

    abstract::A sialic acid binding lectin, AchatininH, was purified in single step from the hemolymph of the land snail, Achatina fulica, by the affinity chromatography on sheep submaxillary mucin coupled to Sepharose 4B. The yield of the lectin was found to be 3 mg from 100 ml of hemolymph. The homogeneity of the lectin was estab...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Basu S,Sarkar M,Mandal C

    更新日期:1986-08-01 00:00:00

  • Splicing factors differentially expressed in psoriasis alter mRNA maturation of disease-associated EDA+ fibronectin.

    abstract::The EDA+ fibronectin splicing variant is overexpressed in psoriatic non-lesional epidermis and sensitizes keratinocytes to mitogenic signals. However, regulation of its abundance is only partially understood. In our recent cDNA microarray experiment, we identified three SR-rich splicing factors-splicing factor, argini...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Szlavicz E,Szabo K,Groma G,Bata-Csorgo Z,Pagani F,Kemeny L,Szell M

    更新日期:2017-12-01 00:00:00

  • Production of nerve growth factor enhanced in cultured mouse astrocytes by glycerophospholipids, sphingolipids, and their related compounds.

    abstract::The NGF secretion from cultured mouse astrocytes was enhanced by sublethal concentrations of phosphatidic acid (PA), ceramide, or sphingosine (Sph), and concentration dependently by lysophosphatidic acid (LPA), sphingosylphosphorylcholine (SPC), or sphingosine-1-phosphate (S1P), but was unaffected by any concentration...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Furukawa A,Kita K,Toyomoto M,Fujii S,Inoue S,Hayashi K,Ikeda K

    更新日期:2007-11-01 00:00:00

  • Human amniotic membrane-derived mesenchymal stem cells labeled with superparamagnetic iron oxide nanoparticles: the effect on neuron-like differentiation in vitro.

    abstract::Mesenchymal stem cells (MSCs) have the potential for self-renewal and multipotential differentiation to regenerate damaged tissues or recover functional absence in diseases. Superparamagnetic iron oxide nanoparticles (SPIONs) are used as contrast agents in magnetic resonance imaging (MRI) for labeling cells in vitro a...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Zeng G,Wang G,Guan F,Chang K,Jiao H,Gao W,Xi S,Yang B

    更新日期:2011-11-01 00:00:00

  • Plasma concentration of intestinal- and liver-FABP in neonates suffering from necrotizing enterocolitis and in healthy preterm neonates.

    abstract::Both early diagnostic and prognostic assessment of the acute abdomen in preterm infants are hampered by the lack of a sensitive and specific parameter for intestinal injury. In this prospective clinical study we wanted to estimate the value of intestinal (I-) and liver (L-) fatty acid binding protein (FABP) in diagnos...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Guthmann F,Börchers T,Wolfrum C,Wustrack T,Bartholomäus S,Spener F

    更新日期:2002-10-01 00:00:00

  • EZH2 promotes cell proliferation by regulating the expression of RUNX3 in laryngeal carcinoma.

    abstract::Enhancer of zeste homolog 2 (EZH2) is a highly conserved histone methyltransferase, which is overexpressed in different types of cancers such as breast and prostate cancer. It is reported that EZH2 can directly down-regulate RUNX3 by increasing histone H3 methylation. However, the role of EZH2 in the development and p...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Lian R,Ma H,Wu Z,Zhang G,Jiao L,Miao W,Jin Q,Li R,Chen P,Shi H,Yu W

    更新日期:2018-02-01 00:00:00

  • The anticancer drug perillyl alcohol is a Na/K-ATPase inhibitor.

    abstract::The monoterpene perillyl alcohol (POH) is a drug used in the treatment of several malignant tumors, including gliomas. The present study defines a POH inhibitory effect on Na/K-ATPase activity from kidney and brain guinea pig extracts and from a human glioblastoma cell line. This inhibition showed a high degree of sel...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Garcia DG,Amorim LM,de Castro Faria MV,Freire AS,Santelli RE,Da Fonseca CO,Quirico-Santos T,Burth P

    更新日期:2010-12-01 00:00:00

  • Potato and rabbit muscle phosphorylases: comparative studies on the structure, function and regulation of regulatory and nonregulatory enzymes.

    abstract::Phosphorylases (EC from potato and rabbit muscle are similar in many of their structural and kinetic properties, despite differences in regulation of their enzyme activity. Rabbit muscle phosphorylase is subject to both allosteric and covalent controls, while potato phosphorylase is an active species without ...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Fukui T,Shimomura S,Nakano K

    更新日期:1982-02-19 00:00:00

  • Expression of stress proteins (HSP-70 and HSP-90) in the rabbit urinary bladder subjected to partial outlet obstruction.

    abstract::Partial obstruction of the rabbit bladder outlet induces a rapid hypertrophy characterized by increased bladder mass, increased smooth muscle content, and increased collagen deposition. In addition, partial outlet obstruction induces decreased contractile responses to both field stimulation and postsynaptic receptor s...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Zhao Y,Chacko S,Levin RM

    更新日期:1994-01-12 00:00:00

  • Up-regulation of the endoplasmic reticulum molecular chaperone GRP78 during hibernation in thirteen-lined ground squirrels.

    abstract::Hibernating mammals endure conditions of low body temperature and oxidative stress that would be highly injurious to humans and most other mammals. Stress conditions frequently trigger the production of molecular chaperones; in the endoplasmic reticulum the glucose-regulated protein-78 (GRP78) helps to minimize protei...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Mamady H,Storey KB

    更新日期:2006-11-01 00:00:00

  • Differential regulation of G-protein expression by vasoactive peptides.

    abstract::Vasoactive peptides such as angiotensin II (AII), atrial natriuretic peptide (ANP) and vasopressin play an important role in the regulation of blood pressure. We have recently shown an augmentation of Gi alpha levels in heart and aorta from genetic and experimentally-induced hypertensive rats, which may be attributed ...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Anand-Srivastava MB,Palaparti A,Pion J

    更新日期:1997-11-01 00:00:00

  • BNIP3 induces IL6 and calcineurin/NFAT3 hypertrophic-related pathways in H9c2 cardiomyoblast cells.

    abstract::Ischemia/reperfusion injury causes cardiomyocyte apoptosis, ventricular remodeling, leading to a dilated heart. Hypoxia is one of the causes involved in ischemia damage, and BNIP3 is a hypoxia-inducible marker and also a sensor to induce mitochondria-dependent apoptosis. Recent reports discussed ablating BNIP3 can res...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Weng YJ,Kuo WW,Kuo CH,Tung KC,Tsai CH,Lin JA,Tsai FJ,Hsieh DJ,Huang CY,Hwang JM

    更新日期:2010-12-01 00:00:00

  • Inhibition of cytosolic and mitochondrial creatine kinase by siRNA in HaCaT- and HeLaS3-cells affects cell viability and mitochondrial morphology.

    abstract::The creatine kinase (CK) system is essential for cellular energetics in tissues or cells with high and fluctuating energy requirements. Creatine itself is known to protect cells from stress-induced injury. By using an siRNA approach to silence the CK isoenzymes in human keratinocyte HaCaT cells, expressing low levels ...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Lenz H,Schmidt M,Welge V,Kueper T,Schlattner U,Wallimann T,Elsässer HP,Wittern KP,Wenck H,Staeb F,Blatt T

    更新日期:2007-12-01 00:00:00

  • Effect of 60 Hz magnetic field exposure on c-fos expression in stimulated PC12 cells.

    abstract::Rat pheochromocytoma PC12 cells have been treated with nerve growth factor (NGF) at final concentrations of 2, 4, 8, and 16 ng/ml, and then were exposed to 60-Hz, sinusoidal magnetic fields (MF) of 12.5, 25, 50, and 100 microT (rms) for 30 min. Transcript levels for both c-fos and glyceraldehyde-3 -phosphate dehydroge...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Campbell-Beachler M,Ishida-Jones T,Haggren W,Phillips JL

    更新日期:1998-12-01 00:00:00

  • Do nitroxides protect cardiomyocytes from hydrogen peroxide or superoxide?

    abstract::The aim of the research was to study the role played by extracellular O2-radicals, which are implicated in cardiac cell damage and the protective effect by cell-permeable, nitroxide, superoxide dismutase-mimics. Cardiomyocytes cultures from 1-day-old rats served as the test-system. Experiments were performed since 5th...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Mohsen M,Pinson A,Zhang R,Samuni A

    更新日期:1995-04-26 00:00:00

  • Mitoapocynin, a mitochondria targeted derivative of apocynin induces mitochondrial ROS generation and apoptosis in multiple cell types including cardiac myoblasts: a potential constraint to its therapeutic use.

    abstract::Mitoapocynin is a triphenylphosphonium conjugated derivative of apocynin that specifically locates to the mitochondria. It has been developed as a mitochondrially targeted therapeutic antioxidant. We attempted to attenuate the mitochondrial ROS induced in H9c2 cardiac myoblast cells treated with norepinephrine. Mitoap...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Mahmood A,Bisoyi P,Banerjee R,Yousuf M,Goswami SK

    更新日期:2021-01-30 00:00:00

  • The up-regulation of cysteine-rich protein 61 induced by transforming growth factor beta enhances osteosarcoma cell migration.

    abstract::Overexpressed cysteine-rich protein 61 (Cyr61) is believed to enhance osteosarcoma (OS) cell metastasis, but the mechanism of Cyr61 overexpression in OS is not clear so far. In this study 33 OS samples were analyzed by immunostaining and focused on two parts: the correlation between overexpression of Cyr61 and OS meta...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Chen J,Song Y,Yang J,Gong L,Zhao P,Zhang Y,Su H

    更新日期:2013-12-01 00:00:00

  • Antioxidant status, lipid peroxidation, mixed function oxidase and UDP-glucuronyl transferase activities in livers from control and DOCA-salt hypertensive male Sprague Dawley rats.

    abstract::The effects of DOCA-salt hypertensive treatment on hepatic glutathione-dependent defense system, antioxidant enzymes, lipid peroxidation, mixed function oxidase and UDP-glucuronyl transferase activities were investigated in male Sprague Dawley rats. Compared with controls, DOCA-salt hypertensive rats had lower body we...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Nicod L,Rodriguez S,Letang JM,Viollon-Abadie C,Jacqueson A,Berthelot A,Richert L

    更新日期:2000-01-01 00:00:00