Abstract：
:We have investigated the links between electrical excitation and contraction in mammalian heart muscle. Using isolated single cells from adult rat ventricle, a whole-cell voltage-clamp technique and quantitative fluorescence microscopy, we have measured simultaneously calcium current (ICa) and [Ca2+]i (with fura-2). We find that the voltage-dependence of ICa and the [Ca2+]i-transient and the dependence of [Ca2+]i-transient on depolarization-duration cannot both be readily explained by a simple calcium-induced Ca-release ('CICR') mechanism. Additionally, we find that when [Ca2+]i and [Na+]i are at their diastolic levels, activation of the Na-Ca exchange mechanism by depolarization does not measurably trigger the release of Ca2+i. Finally, measuring ICa in adult and neonatal rat heart cells and using the alkaloid ryanodine, we have carried out complementary experiments. These experiments show that there may be an action of ryanodine on ICa that is independent of [Ca2+]i and independent of a direct action of the alkaloid on the calcium channel itself. Along with experiments of others showing that ryanodine binds to the sarcoplasmic reticulum calcium-release channel/spanning protein complex, our data suggests a model to explain our findings. The model links the calcium channels responsible for ICa to the sarcoplasmic reticulum by means of one or more of the spanning protein(s). Information from the calcium channel can be communitated to the sarcoplasmic reticulum by this route and, presumably, information can move in the opposite direction from the sarcoplasmic reticulum to the calcium channel.
journal_name
Mol Cell Biochemjournal_title
Molecular and cellular biochemistryauthors
Lederer WJ,Cannell MB,Cohen NM,Berlin JRdoi
10.1007/BF00220762subject
Has Abstractpub_date
1989-09-07 00:00:00pages
115-9issue
2eissn
0300-8177issn
1573-4919journal_volume
89pub_type
杂志文章abstract：:A proliferation-inducing ligand (APRIL) is overexpressed in most tumor cells and tissues, especially in tumors of the alimentary system, such as colorectal cancer (CRC), gastric cancer, and liver cancer. RNA interference (RNAi) has been proved to be a powerful tool for gene knockdown and holds great promise for the tr...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-011-1151-4
更新日期：2012-04-01 00:00:00
abstract：:Succinic acid monoethyl ester (EMS) is recently proposed as an insulinotropic agent for the treatment of non-insulin dependent diabetes mellitus. Oxidative stress has been suggested to be a contributory factor in the development and complications of diabetes. In the present study the effect of EMS and Metformin on pla...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-006-9312-6
更新日期：2007-02-01 00:00:00
abstract：:We studied the effects of SEB on [14C]-choline transport and metabolism of choline containing phospholipids in cultured human kidney proximal tubular (PT) cells. SEB increased the uptake of [14C]-choline in PT cells as a function of toxin concentration, incubation time, and pH. The maximum increase in uptake (3.5-5-fo...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/BF00944603
更新日期：1995-05-24 00:00:00
abstract：:Atherosclerosis is the leading cause of death in North America. It is characterized by thickening of the coronary artery wall by the formation of plaques, resulting in reduced blood flow. Plaque rupture and the consequent thrombosis may lead to sudden blockage of arteries and causing stroke and heart attack. In the la...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章,评审
doi：10.1023/a:1017286006708
更新日期：2000-04-01 00:00:00
abstract：:In hypertensive animals and patients, oxidative stress represents the primary risk factor for progression of left ventricular hypertrophy. Recently, it has been demonstrated that hydrogen, as a novel antioxidant, can selectively reduce hydroxyl radicals and peroxynitrite anion to exert therapeutic antioxidant activity...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-012-1264-4
更新日期：2012-06-01 00:00:00
abstract：:Oxidative stress is one of the mechanisms involved in the acute carbon tetrachloride (CCl4)-induced hepatotoxicity. Since 4-hydroxy-2,2,6,6-tetramethylpiperidin-1-oxyl, known as tempol, has powerful antioxidant properties, we investigated its potential hepatoprotective effects and the underlying mechanisms that may ad...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-015-2572-2
更新日期：2016-01-01 00:00:00
abstract：:Liver glucose 6-phosphate dehydrogenase and phosphogluconate dehydrogenase activities were significantly decreased in both diabetic and fasted rats. Treatment of diabetic rats with insulin resulted in liver glucose 6-phosphate dehydrogenase and phosphogluconate dehydrogenase activities that were significantly greater ...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/BF00229431
更新日期：1986-05-01 00:00:00
abstract：:The interaction between dipalmitoyl lecithin and egg lecithin with insulin was studied in a non-aqueous solvent such as dioxane-chloroform (1:1) by dielectric constant measurements and absorption spectra. The electrostatic character of the interaction results from the dielectric measurements and the effect of an exter...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/BF00215300
更新日期：1980-03-20 00:00:00
abstract：:Many recent studies have suggested that bergapten (BP), a class of native compound with numerous biological activities such as anti-resorptive properties, may exert protective effects against postmenopausal bone loss. However, it remains unknown whether BP regulates or improves the osteogenic function of bone marrow s...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-015-2517-9
更新日期：2015-11-01 00:00:00
abstract：:We investigated whether chronic in vivo treatment with the peroxisome proliferator-activated receptor alpha agonist Wy-14,643 attenuates cardiac contractile function by impairing mitochondrial respiration. Wy-14,643 (25 mg kg(-1) day(-1)) was administered to Wistar rats by oral gavage for 14 consecutive days, after wh...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-009-0100-y
更新日期：2009-10-01 00:00:00
abstract：:The binding of nuclear factor on the promoter region of the regucalcin gene and the expression of regucalcin in the kidney cortex of rats was investigated. Nuclear extracts from kidney cortex were used for oligonucleotide competition gel mobility shift assay. An oligonucleotide between position -523 and -506 in the 5'...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1023/a:1011040805597
更新日期：2001-03-01 00:00:00
abstract：:Cross-talk between coding RNAs and regulatory non-coding microRNAs, within human genome, has provided compelling evidence for the existence of flexible checkpoint control of T-Cell activation. The present study attempts to demonstrate that the interplay between miR-2909 and its effector KLF4 gene has the inherent capa...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-018-3390-0
更新日期：2019-01-01 00:00:00
abstract：:The PPAR-gamma receptor agonists, as a relatively new and perhaps still not very widely used class of antidiabetic agent in the Caribbean and particularly the Trinidadian context, possess pharmacologic properties that certainly have been shown to have impact on many of the inflammatory, metabolic, biochemical and stru...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章,评审
doi：
更新日期：2004-08-01 00:00:00
abstract：:The heptahelical lutropin receptor (LHR) signals primarily via the Gs-adenylyl cyclase pathway and undergoes ligand-mediated receptor desensitization and internalization. A loss-of-function rat LHR mutant was recently described in which a single amino acid residue replacement in exoloop 3, K583E, had no effect on huma...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1023/a:1006816401109
更新日期：1998-10-01 00:00:00
abstract：:An arginase isolated from a capsulated Bacillus anthracis strain was highly purified and crystallized. The chemical and immunological characteristics of this enzyme re described. Some very important properties differ from those of another bacterial arginase, i.e. Staphylococcus aureus arginase, described in a previous...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/BF00285642
更新日期：1983-01-01 00:00:00
abstract：:Silkworm (Bombyx mori) is not only a model organism for scientific studies, but also a commercial insect for agricultural production. BmAtg8 (a B. mori homolog of yeast Atg8) plays crucial roles in macroautophagy (hereafter referred to autophagy), which is helpful for silkworm metamorphosis. Relevant mechanism about B...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-019-03513-y
更新日期：2019-07-01 00:00:00
abstract：:MicroRNAs (miRNAs) are small non-coding RNAs that have been suggested to play an essential role in tumorigenesis. Reduced expression of miR-338 has been reported in several types of cancers; however, the role of miR-338 in oral squamous cell carcinoma (OSCC) has not been elucidated. In this study, we demonstrated that...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-014-2211-3
更新日期：2015-01-01 00:00:00
abstract：:There is general agreement that the connexin43 gap junction protein is a substrate for phosphorylation by protein kinase C but there is no similar consensus regarding the action of protein kinase A. Our previous studies demonstrated that channels formed by connexin43 were reversibly gated in response to microinjected ...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1023/a:1019902920693
更新日期：2002-09-01 00:00:00
abstract：:In addition of being an important inflammatory biomarker and a risk factor for cardiovascular disease, much evidence indicates that the C-reactive protein (CRP) contributes to the atherosclerosis development process. This plasmatic protein synthesized by hepatocytes in response to inflammation and tissue injury induce...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-012-1482-9
更新日期：2013-01-01 00:00:00
abstract：:Mitogen-activated protein kinase (MAPK) and renin-angiotensin system (RAS) play critical roles in the process of renal diseases, but their interaction has not been comprehensively discussed. In the present studies, we investigated the renoprotective effects of MPAK inhibitors on renal diseases in type 1 diabetic mouse...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-016-2722-1
更新日期：2016-08-01 00:00:00
abstract：:Accumulating data have shown that microRNAs are involved in the pathogenesis of cancer. miR-202 has been confirmed to be downregulated in several types of human cancer. However, the expression and biological role of miR-202 in osteosarcoma (OS) carcinogenesis and progression remain unclear. In this study, we demonstra...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-014-2195-z
更新日期：2014-12-01 00:00:00
abstract：:Both early diagnostic and prognostic assessment of the acute abdomen in preterm infants are hampered by the lack of a sensitive and specific parameter for intestinal injury. In this prospective clinical study we wanted to estimate the value of intestinal (I-) and liver (L-) fatty acid binding protein (FABP) in diagnos...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：
更新日期：2002-10-01 00:00:00
abstract：:Doxorubicin (DOX) is a DNA topoisomerase II inhibitor widely used in anticancer treatment, however, it can lead to irreversible cardiac damage with severe debilitation. TBP-binding associated factor 1 (TAF1) is increased in DOX damaged hearts in vivo and in cardiomyocytes in vitro. To identify the functional role for ...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1023/b:mcbi.0000021347.65073.10
更新日期：2004-04-01 00:00:00
abstract：:Hypertension and related oxidative stress are involved in the pathogenesis of any renal diseases. Angiotensin-converting enzyme inhibitors have multi-directional renoprotective effects. In this study, we aimed to investigate whether lisinopril treatment has any biochemical alterations on renal tissue in L-NAME (Nε-nit...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-011-0760-2
更新日期：2011-06-01 00:00:00
abstract：BACKGROUND:We reported that urinary L-FABP reflected the progression of chronic kidney disease (CKD). This study is aimed to evaluate the clinical significance of urinary liver type fatty acid binding protein (L-FABP) as a biomarker for monitoring CKD. METHODS:Urinary L-FABP was measured using human L-FABP ELISA kit (...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-005-9047-9
更新日期：2006-03-01 00:00:00
abstract：:Genistein is often used as an inhibitor of tyrosine kinases. A less studied side effect of genistein is an inhibition of cyclic AMP-phosphodiesterase (cAMP-PDE) activity resulting in increased cAMP accumulation. The effect of genistein on intracellular cAMP-levels, basal and forskolin-induced, was studied in A549 huma...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1023/a:1020655724911
更新日期：2002-11-01 00:00:00
abstract：:Heat-shock protein90 (HSP90) plays an essential role in maintaining stability and activity of its clients. HSP90 is involved in cell differentiation and survival in a variety of cell types. To elucidate the possible role of HSP90 in myogenic differentiation and cell survival, we examined the time course of changes in ...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-011-0977-0
更新日期：2011-12-01 00:00:00
abstract：:Fatty acid uptake and metabolism are often dysregulated in cancer cells. Fatty acid activation is a critical step that allows these biomolecules to enter cellular metabolic pathways such as mitochondrial β-oxidation for ATP generation or the lipogenic routes that generate bioactive lipids such as the inositol phosphol...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-018-3332-x
更新日期：2018-11-01 00:00:00
abstract：:NLRX1, the mitochondrial NOD-like receptor (NLR), modulates apoptosis in response to both intrinsic and extrinsic cues. Insights into the mechanism of how NLRX1 influences apoptosis remain to be determined. Here, we demonstrate that NLRX1 associates with SARM1, a protein with a toll/interleukin-1 receptor (TIR)-contai...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-018-3444-3
更新日期：2019-03-01 00:00:00
abstract：:Gentamicin (GM) is an antibiotic whose clinical use is limited by its nephrotoxicity. Experimental evidences suggest a role of reactive oxygen species in GM-induced nephrotoxicity. In this work we explored the effect of diallyl sulfide (DAS), a garlic-derived compound with antioxidant properties, on GM-induced nephrot...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1023/a:1027372102135
更新日期：2003-12-01 00:00:00
