Abstract：
:The heptahelical lutropin receptor (LHR) signals primarily via the Gs-adenylyl cyclase pathway and undergoes ligand-mediated receptor desensitization and internalization. A loss-of-function rat LHR mutant was recently described in which a single amino acid residue replacement in exoloop 3, K583E, had no effect on human choriogonadotropin (hCG) binding but essentially abolished signaling. This LHR mutant is a prime candidate for which to study hCG-mediated receptor internalization since it is highly unlikely that an amino acid residue in exoloop 3 , i.e. an extracellular portion of LHR connecting transmembrane helices 6 and 7, could have any direct interaction with Galpha(s), which is located on the cytoplasmic face of the plasma membrane. A method to study endocytosis was adapted that involves concanavalin A binding to the glycoproteins on the cell surface, thus facilitating separation of the plasma membrane fraction from other cellular membrane fractions by sucrose gradient centrifugation. Conditions were used such that a single round of endocytosis could be determined with [125I]hCG. Endocytic rate constants of 0.03 and O min(-1) were obtained for LHR and the mutant, respectively, in transfected human embryonic kidney 293 cells; moreover, internalization of the mutant could not be restored by the addition of 8-Br-cAMP. Thus, the presence of the second messenger cAMP is not sufficient for internalization of ligand-occupied LHR. Rather, it appears that ligand-mediated activation and subsequent internalization of LHR results from an altered conformational state or a conformation-dependent post-ligand binding modification such as phosphorylation.
journal_name
Mol Cell Biochemjournal_title
Molecular and cellular biochemistryauthors
Bhowmick N,Narayan P,Puett Ddoi
10.1023/a:1006816401109subject
Has Abstractpub_date
1998-10-01 00:00:00pages
221-7issue
1-2eissn
0300-8177issn
1573-4919journal_volume
187pub_type
杂志文章abstract：:The treatment of obstructive sleep apnea-hypopnea syndrome targets the narrow anatomic structure of the upper airway (UA) and lacks an effective therapy for UA dilator muscle dysfunction. Long-term hypoxia can cause damage to UA dilator muscles and trigger a vicious cycle. We previously confirmed that hypoxia-inducibl...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-020-03995-1
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-010-0649-5
更新日期：2011-02-01 00:00:00
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-007-9540-4
更新日期：2007-11-01 00:00:00
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-006-9234-3
更新日期：2006-12-01 00:00:00
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章,评审
doi：
更新日期：1998-11-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s11010-018-3410-0
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doi：10.1007/s11010-006-9312-6
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doi：10.1007/s11010-017-3242-3
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-006-9137-3
更新日期：2006-08-01 00:00:00
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/BF00230325
更新日期：1992-10-07 00:00:00
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pub_type： 杂志文章
doi：10.1007/BF00242520
更新日期：1988-07-01 00:00:00
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-011-1002-3
更新日期：2012-01-01 00:00:00
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/BF00229312
更新日期：1996-02-23 00:00:00
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1023/a:1006979606225
更新日期：1999-07-01 00:00:00
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-016-2684-3
更新日期：2016-05-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s11010-015-2548-2
更新日期：2015-12-01 00:00:00
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/BF00219255
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pub_type： 临床试验,杂志文章
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/BF00925951
更新日期：1994-02-23 00:00:00
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journal_title：Molecular and cellular biochemistry
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更新日期：1996-09-20 00:00:00
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pub_type： 杂志文章
doi：10.1007/s11010-013-1732-5
更新日期：2013-10-01 00:00:00
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/BF01837058
更新日期：1976-11-30 00:00:00
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-008-9784-7
更新日期：2008-08-01 00:00:00
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/BF00944389
更新日期：1995-03-23 00:00:00
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章,评审
doi：10.1007/BF00225255
更新日期：1983-01-01 00:00:00
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
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更新日期：2015-05-01 00:00:00
