Abstract：
:Frequenin is a member of the neuronal Ca(2+) sensor protein family, implicated in being the modulator of the neurotransmitter release, potassium channels, phosphatidylinositol signaling pathway and the Ca(2+)-dependent exocytosis of dense-core granules in the PC12 cells. Frequenin exhibits these biological activities through its Ca(2+) myristoyl switch, yet the switch is functionally inactive. These structural and functional traits of frequenin have been derived through the use of recombinant frequenin. In the present study, frequenin (BovFrq) native to the bovine hippocampus has been purified, sequenced for its 9 internal fragments, cloned, and studied. The findings show that structure of the BovFrq is identical to its form present in chicken, rat, mouse and human, indicating its evolutionary conservation. Its Ca(2+) myristoyl switch is active in the hippocampus. And, BovFrq physically interacts and turns on yet undisclosed ONE-GC-like ROS-GC membrane guanylate cyclase transduction machinery in the hippocampal neurons. This makes BovFrq a new Ca(2+)-sensor modulator of a novel ROS-GC transduction machinery. The study demonstrates the presence and mechanistic features of this cyclic GMP signaling pathway in the hippocampal neurons, and also provides one more support for the evolving concept where the Ca(2+)-modulated membrane guanylate cyclase transduction machinery in its variant forms is a central operational component of all neurons.
journal_name
Mol Cell Biochemjournal_title
Molecular and cellular biochemistryauthors
Fik-Rymarkiewicz E,Duda T,Sharma RKdoi
10.1007/s11010-006-9215-6subject
Has Abstractpub_date
2006-10-01 00:00:00pages
187-204issue
1-2eissn
0300-8177issn
1573-4919journal_volume
291pub_type
杂志文章abstract：:In this study, we have characterized the negative regulation of the IL-12 p40 expression by TGF-beta in macrophages. Although murine IL-12 p40 promoter contains a putative TGF-beta inhibitory element (TIE), neither mutation nor deletion of the TIE had any effect on the inhibitory activity of TGF-beta. The NF-kappaB p6...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-007-9605-4
更新日期：2008-01-01 00:00:00
abstract：:Chronic aristolochic acid (AA) nephropathy (CAAN) caused by intake of AA-containing herbs is difficult to treat. We evaluated the therapeutic effect of bone marrow (BM) mesenchymal stem cells (MSCs) on a rat model of CAAN. Female Wistar rats were fed with decoction of Caulis Aristolochia manshuriensis by intragastric ...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-012-1352-5
更新日期：2012-09-01 00:00:00
abstract：:To differentiate whether the primary volume signal in dog red cells arises from a change in cell configuration or the concentration and dilution of cell contents, we prepared resealed ghosts that had the same surface area and hemoglobin concentration as intact cells but less than 1/3 their volume. Shrinkage of both in...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/BF00240291
更新日期：1992-09-08 00:00:00
abstract：:Studies were carried out on glucose-6-phosphate dehydrogenase (G6P-DH) during the differentiation of rabbit bone marrow erythroid cells. It was found that G6P-DH, although displaying a 7-fold activity decrease, did not change the relative amounts of its three dimeric forms. Using homogeneous enzyme preparations, we ob...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/BF00229896
更新日期：1987-06-01 00:00:00
abstract：:This paper reviews characteristics of microsomal membrane structure; long chain fatty acids, acyl CoA derivatives, retinoids and the microsomal formation of acyl CoA derivatives and retinyl esters. It is analyzed how the movement of these molecules at the intracellular level is affected by their respective binding pro...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章,评审
doi：10.1007/BF00926080
更新日期：1993-03-24 00:00:00
abstract：:Glycosphingolipid biosynthesis was examined using [3H]-galactose as a precursor as rat L6 myoblasts fused to form multinucleated myotubes. Incorporation of label into neutral glycolipids decreased steadily as the population of myotubes increased, so that final biosynthesis was one-half that observed with myoblasts (p ...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/BF00223197
更新日期：1988-09-01 00:00:00
abstract：:Retinal pigment epithelium (RPE) exerts critical roles in the maintenance of the normal functions of the retina, whereas RPE dysfunction can induce retina neovascularization. p75 neurotrophin receptor (p75(NTR)) has been shown to play essential roles in angiogenesis. However, the function of p75(NTR) in the RPE remain...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-014-2212-2
更新日期：2015-01-01 00:00:00
abstract：:Hepatocyte cell adhesion molecule (HepaCAM) plays a crucial role in tumor progression and has been recognized as a novel tumor suppressor gene. The high protein expression level of protein kinase Cε (PKCε) has been discovered in many tumor types. In the present study, we determined HepaCAM and PKCε protein levels in h...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-014-1991-9
更新日期：2014-06-01 00:00:00
abstract：:The aim of the study was to find out whether administration of selenium (Se) will protect the immature heart against ischemia/reperfusion.The control pregnant rats were fed laboratory diet (0.237 mg Se/kg diet); experimental rats received 2 ppm Na(2)SeO(3) in the drinking water from the first day of pregnancy until da...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-006-9391-4
更新日期：2007-06-01 00:00:00
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：
更新日期：1999-01-01 00:00:00
abstract：:Sarcolemmal Ca2+/Mg2+ ecto-ATPase (Myoglein; MW 180 kD) is a membrane bound enzyme which requires a millimolar concentration of either Ca2+ or Mg2+ for maximal hydrolysis of ATP. The isoelectric point (pI) of the cardiac ecto-ATPase was 5.7. The purified Ca2+/Mg2+ ecto-ATPase from the rat heart sarcolemmal appeared as...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1023/a:1006840523916
更新日期：1998-06-01 00:00:00
abstract：:Abnormal QT prolongation with the associated arrhythmias is a significant predictor of mortality in diabetic patients. Gap junctional intercellular communication allows electrical coupling between heart muscle cells. The effects of streptozotocin (STZ)-induced diabetes mellitus on the expression and distribution of co...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-008-9883-5
更新日期：2008-12-01 00:00:00
abstract：:The effects of different dietary fats on peritoneal macrophage plasma membrane fluidity, intracellular cyclic AMP (cAMP) production, GTP hydrolysis and TNF binding and TNF-induced IL1 and IL6 production was investigated. After a four week period, fluidity, as determined by both fluorescence recovery after photobleachi...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1023/a:1006875010120
更新日期：1997-01-01 00:00:00
abstract：:Sinomenine, a pure alkaloid extract from Sinomenium acutum, has anti-inflammatory and immunoregulatory functions. This study investigated the efficiency and the signalling pathways involved in the effect of sinomenine on vascular smooth muscle cell (VSMC) dedifferentiation in response to platelet-derived growth factor...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-012-1474-9
更新日期：2013-01-01 00:00:00
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/BF00223195
更新日期：1988-09-01 00:00:00
abstract：:The pathophysiologic importance of insulin resistance in diseases such as obesity and diabetes mellitus has led to great interest in defining the mechanism of insulin action as well as the means to overcome the biochemical defects responsible for the resistance. Vanadium compounds have been discovered to mimic many of...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章,评审
doi：
更新日期：1998-05-01 00:00:00
abstract：:Ionizing radiation induces apoptosis in human Molt-4 leukemia cells in a p53-dependent manner. The tumor suppressor p53 stimulates various downstream targets that presumably trigger, individually or in concert, de novo ceramide synthesis and intrinsic apoptosis via mitochondrial outer membrane permeabilization (MOMP)....
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-020-03874-9
更新日期：2020-12-01 00:00:00
abstract：:It is well established that severe hypertrophy induces metabolic and structural changes in the heart which result in enhanced susceptibility to ischemic damage during cardioplegic arrest while much less is known about the effect of cardioplegic arrest on moderately hypertrophied hearts. The aim of this study was to el...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：
更新日期：1998-03-01 00:00:00
abstract：:The heptahelical lutropin receptor (LHR) signals primarily via the Gs-adenylyl cyclase pathway and undergoes ligand-mediated receptor desensitization and internalization. A loss-of-function rat LHR mutant was recently described in which a single amino acid residue replacement in exoloop 3, K583E, had no effect on huma...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1023/a:1006816401109
更新日期：1998-10-01 00:00:00
abstract：:Cardiac hypertrophy is an independent risk factor in the development of heart failure. However, the cellular mechanisms underlying the transition from compensated hypertrophy to heart failure are incompletely understood. The aim of this study was to investigate changes in myocardial substrate utilisation and function ...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-008-9711-y
更新日期：2008-04-01 00:00:00
abstract：:We have observed that phospholipids and protein of the catecholamine (CA) storage granules, i.e. the chromaffin granules, interact in an in vitro system to form liposomal particles, which in many respects resemble the intact matrix of the bovine chromaffin granule. A model has been suggested which consists of an aqueo...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章,评审
doi：10.1007/BF01732006
更新日期：1975-02-28 00:00:00
abstract：:Sirtuin 3 (SIRT3) modulates mitochondria-localized processes and is implicated in the metabolic reprogramming of cancer cells, especially fatty acid (FA) synthesis. However, the relationship between SIRT3 and aberrant lipid synthesis in cervical cancer remains unclear. Here, we investigated the clinical relevance of S...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-019-03644-2
更新日期：2020-01-01 00:00:00
abstract：:Hepatocellular carcinoma (HCC) is one of leading causes of cancer-related death, and its increasing incidence worldwide is a cause for concern. The recombinant analgesic-antitumor peptide (rAGAP), a protein consisting of small ubiquitin-related modifier linked with a hexa-histidine tag, exhibited the antitumor activit...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-018-3475-9
更新日期：2019-05-01 00:00:00
abstract：:Reactive oxygen species (ROS) and reactive nitrogen species (RNS) are highly reactive transient chemical species, which play an important role in the etiology of tissue injury in rheumatoid arthritis (RA). The effects of milk extract of Semecarpus anacardium Linn. nut (SA) was studied on adjuvant arthritis in rats. Ar...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-005-3202-1
更新日期：2005-08-01 00:00:00
abstract：:The effects of infusing superoxide dismutase (SOD) and catalase (CAT) into the coronary circulation were investigated in isolated, working rat hearts prior to and during a 15 minute episode of regional ischemia followed by 30 minutes reperfusion. Aortic output, left ventricular pressure and dP/dT were recorded. Compar...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/BF00420901
更新日期：1990-08-10 00:00:00
abstract：:Human cardiomyopathy has been extensively studied in the last decade, and knowledge of the functional and structural alterations of the heart has grown. However, understanding of the pathogenesis has come mostly from experimental studies. A number of work have been designed to elucidate if alterations of the contracti...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章,评审
doi：10.1007/978-1-4615-2612-4_19
更新日期：1994-04-01 00:00:00
abstract：:The natriuretic peptide receptor-A (NPR-A) is involved in blood pressure and body fluid regulation in order to help maintain cardiovascular homeostasis. It has been shown that these biological effects are mediated through the natriuretic peptide family of hormones, which bind NPR-A according to the rank order ANP>BNP>...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1023/a:1006835808554
更新日期：1999-04-01 00:00:00
abstract：:Obesity-induced low-grade inflammation (metaflammation) impairs insulin receptor signaling. This has been implicated in the development of insulin resistance. Insulin signaling in the target tissues is mediated by stress kinases such as p38 mitogen-activated protein kinase, c-Jun NH2-terminal kinase, inhibitor of NF-k...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章,评审
doi：10.1007/s11010-016-2878-8
更新日期：2017-02-01 00:00:00
abstract：:Endochondral ossification, an important stage of fracture healing, is regulated by a variety of signaling pathways. Transforming growth factor β (TGFβ) superfamily plays important roles and comprises TGFβs, bone morphogenetic proteins (BMPs), and growth differentiation factors. TGFβs primarily regulate cartilage forma...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-014-2173-5
更新日期：2014-12-01 00:00:00
abstract：:In mammalian cells, increases in calcium concentration cause increases in oxidative phosphorylation. This effect is mediated by the activation of four mitochondrial dehydrogenases by calcium ions; FAD-glycerol 3-phosphate dehydrogenase, pyruvate dehydrogenase, NAD-isocitrate dehydrogenase and oxoglutarate dehydrogenas...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/BF01076578
更新日期：1995-08-01 00:00:00
