当前位置： SCI文献检索 > MOLECULAR AND CELLULAR BIOCHEMISTRY期刊下所有文献 > Methylation of the promoter A of estrogen receptor alpha gene in hBMSC and osteoblasts and its correlation with homocysteine.

Methylation of the promoter A of estrogen receptor alpha gene in hBMSC and osteoblasts and its correlation with homocysteine.

Abstract：

:Recent studies have shown that adult osteoporosis could be induced by ageing and estrogen deficiency and homocysteine (Hcy) is an independent risk factor of fracture in osteoporosis patients. In this study, we found hypermethylation of the promoter A region in the estrogen receptor alpha (ERα) gene, and methylation in 70.59% of 68 post-menopausal women, whose methylation degree was significantly higher than the pre-menopausal women (P < 0.05). Their methylation frequency was detected only 26.67% in 30 subjects. An obvious correlation between the degree of methylation in ERα gene and the level of Hcy (r = 0.809, P < 0.05) was explored. The cultured human bone marrow strom cells (hBMSC) and osteoblasts treated by Hcy resulted in de novo methylation of the promoter A region in the ERα gene and suppressed proliferation and differentiation with time and dose dependence. Meanwhile, ERα gene mRNA in osteoblast-like cells treated by Hcy was much lower than the control group (P < 0.05). Thus, both in vivo and in vitro data showed that Hcy could promote hypermethylation of the promoter A region and reduce ERα mRNA transcription, which may be an important mechanism for the pathogenesis of postmenopausal osteoporosis.

journal_name

Mol Cell Biochem

journal_title

Molecular and cellular biochemistry

authors

Lv H,Ma X,Che T,Chen Y

doi

10.1007/s11010-011-0836-z

subject

Has Abstract

pub_date

2011-09-01 00:00:00

pages

35-45

issue

1-2

eissn

0300-8177

issn

1573-4919

journal_volume

355

pub_type

杂志文章

相关文献

MOLECULAR AND CELLULAR BIOCHEMISTRY文献大全
  • Up-regulation of PCSK9 gene expression and diminished level of LDL-receptor in rat liver as a potential cause of post-lipectomy hypercholesterolemia.

    abstract：:Studies designed to examine effects of fat mass reduction (including lipodystrophy and lipectomy) on human serum total and LDL-cholesterol concentrations are inconsistent. The purpose of this study was to examine effect of partial lipectomy in rats (as an experimental model of fat mass reduction in humans) on (1) circ...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-018-3484-8

    authors： Dettlaff-Pokora A,Sucajtys-Szulc E,Sledzinski T

    更新日期：2019-05-01 00:00:00

  • Genetic polymorphism of CYP3A5 in Indian chronic myeloid leukemia patients.

    abstract：:CYP3A5 is an important genetic contributor to inter-individual differences in CYP3A-dependent clinically important drugs of metabolism and also of various endogenous compounds and environmental contaminants. The CYP3A5*3 allele results in a truncated protein with loss of CYP3A5 expression and CYP3A5*6 is associated wi...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-009-0268-1

    authors： Bajpai P,Tripathi AK,Agrawal D

    更新日期：2010-03-01 00:00:00

  • ADP-ribosylation factors: a family of approximately 20-kDa guanine nucleotide-binding proteins that activate cholera toxin.

    abstract：:ADP-ribosylation factors (ARFs) comprise a family of approximately 20 kDa guanine nucleotide-binding proteins that were discovered as one of several cofactors required in cholera toxin-catalyzed ADP-ribosylation of Gs alpha, the guanine nucleotide-binding protein responsible for stimulation of adenylyl cyclase, and wa...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/BF00928458

    authors： Welsh CF,Moss J,Vaughan M

    更新日期：1994-09-01 00:00:00

  • Characteristics of an ADP receptor mediating platelet activation.

    abstract：:Adenosine diphosphate (ADP) is known to induce platelet shape change, aggregation and fibrinogen binding, followed by secretion. These processes are mediated by the binding of ADP to an externally oriented protein of the platelet plasma membrane. An affinity analog of ATP, a competitive inhibitor of the action of ADP,...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章,评审

    doi：10.1007/BF00231307

    authors： Colman RW,Figures WR

    更新日期：1984-01-01 00:00:00

  • The mechanism of biogenesis and potential function of the two alternatively spliced mRNAs encoded by the murine Msx3 gene.

    abstract：:The homeodomain-containing Msx3 gene, the newest member of the Msx family, encodes two mRNAs, with an unknown relationship to each other. To elucidate how Msx3 gene generates the two transcripts, we cloned their corresponding cDNAs from an E10.5 mouse embryo cDNA library. The alignment of sequences of the two Msx3-spe...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1023/b:mcbi.0000049353.38098.51

    authors： Matsui H,Takahashi T,Raghow R

    更新日期：2004-12-01 00:00:00

  • Inhibition of glutathione S-transferase-pi triggers c-jun N-terminal kinase-dependent neuronal death in Zn-induced Parkinsonism.

    abstract：:Oxidative stress is recognized as one of the major wrongdoers in Parkinson's disease (PD) while glutathione S-transferase (GST), an endogenous antioxidant, protects from oxidative stress-induced neurodegeneration. Despite GST-pi (GST-π) encounters the toxic manifestations in PD, its role in zinc (Zn)-induced nigrostri...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-018-3415-8

    authors： Chauhan AK,Mittra N,Singh BK,Singh C

    更新日期：2019-02-01 00:00:00

  • Characterization of gastrin binding to colonic mucosal membranes of guinea pigs.

    abstract：:Gastrin has significant growth and metabolic effects on colonic mucosal cells. It is, however, not known if gastrin receptors are present on colonic mucosal cells that may directly mediate the reported biological effects of gastrin. In the present studies, the presence of specific gastrin binding sites on colonic muco...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/BF00227573

    authors： Narayan S,Chicione L,Singh P

    更新日期：1992-06-26 00:00:00

  • Effect of chronic colchicine administration on the myocardium of the aging spontaneously hypertensive rat.

    abstract：:Colchicine has been demonstrated to suppress the release of fibroblast growth factors, retard collagen formation and augment collagenase activity. Trials with colchicine in patients with hepatic fibrosis have suggested clinical benefit. The development of impaired myocardial function in the spontaneously hypertensive ...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1023/a:1006889126666

    authors： Cicogna AC,Brooks WW,Hayes JA,Robinson KG,Sen S,Conrad CH,Bing OH

    更新日期：1997-01-01 00:00:00

  • Functional analysis of miRNAs combined with TGF-β1/Smad3 inhibitor in an intrauterine rat adhesion cell model.

    abstract：:In this study, we aimed to study the role of miRNAs in intrauterine adhesion (IUA) disease. An IUA cell model was constructed by TGF-β1. Smad3 inhibitor (SIS3) can inhibit the Smad3 signaling pathway and affect the role of TGF-β1; thus, it was used to identify the role of Smad3 and related miRNAs in IUA. Cell number s...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-020-03741-7

    authors： Liu S,Huang X,Liu Y,Song D,Xiao Y

    更新日期：2020-07-01 00:00:00

  • Direct localisation of molecules in tissue sections of growing antler tips using MALDI imaging.

    abstract：:The astonishing growth rate of deer antlers offers a valuable model for the discovery of novel factors and regulatory systems controlling rapid tissue growth. Numerous molecules have been identified in growing antlers using a variety of techniques. However, little is known about the spatial distribution of these molec...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-015-2527-7

    authors： Deb-Choudhury S,Wang W,Clerens S,McMahon C,Dyer JM,Li C

    更新日期：2015-11-01 00:00:00

  • Receptor-Ck-dependent regulation of genes involved in the cell cycle.

    abstract：:The present study was addressed to understand the interrelationship between Receptor-Ck activation, mevalonate pathway and primary response genes such as c-fos, c-myc and cyclin 'D' involved in the cell cycle. The results reported here unambiguously revealed that the phosphatidic acid (generated through the activation...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1023/a:1006890215502

    authors： Kaur M,Kaul D,Sobti RC

    更新日期：1998-04-01 00:00:00

  • Effects of N-(2-mercaptopropionyl)glycine on ischemic-reperfused dog kidney in vivo and membrane preparation in vitro.

    abstract：:The results of our experiments demonstrated that one hour of ischemia followed by one hour of reflow in the kidney caused a reduction in (Na+K+)ATPase activity and microsomal sulfhydryl content as well as an increase in microsomal lipid peroxidation. Renal venous malondialdehyde concentration was increased soon after ...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/BF00229689

    authors： Kato M,Kako KJ

    更新日期：1987-12-01 00:00:00

  • Comparison of the thermolability and hydrophobic properties of high- and low-molecular-weight forms of rabbit liver arginyl-tRNA synthetase.

    abstract：:Two preparations with arginyl-tRNA synthetase activity have been obtained from rabbit liver post-microsomal fraction: a) a high-molecular-weight containing the multienzyme aminoacyl-tRNA synthetase complex and b) a low-molecular-weight preparation containing free enzymes. Thermal inactivation of arginyl-tRNA synthetas...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/BF00222612

    authors： Berbeć H,Paszkowska A

    更新日期：1989-04-11 00:00:00

  • Mechanism of attenuation by beta-hydroxy-beta-methylbutyrate of muscle protein degradation induced by lipopolysaccharide.

    abstract：:The mechanism of the effect of beta-hydroxy-beta-methylbutyrate (HMB) on protein degradation induced by lipopolysaccharide (LPS) has been evaluated in murine myotubes. HMB (50 muM) completely attenuated total protein degradation induced by LPS (1-100 ng/ml), formation of reactive oxygen species (ROS) and activation of...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-009-0130-5

    authors： Russell ST,Tisdale MJ

    更新日期：2009-10-01 00:00:00

  • Metabolic phenotyping of the diseased rat heart using 13C-substrates and ex vivo perfusion in the working mode.

    abstract：:The objective of the present study was to compare energy substrate fluxes through metabolic pathways leading to mitochondrial citrate synthesis and release in normal and diseased rat hearts using 13C-substrates and mass isotopomer analysis by gas chromatography-mass spectrometry (GCMS). This study was prompted by our ...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：

    authors： Vincent G,Khairallah M,Bouchard B,Des Rosiers C

    更新日期：2003-01-01 00:00:00

  • Dependency of microtubule-associated proteins (MAPs) for tubulin stability and assembly; use of estramustine phosphate in the study of microtubules.

    abstract：:Microtubule-associated proteins (MAPs) were separated from tubulin with several different methods. The ability of the isolated MAPs to reinduce assembly of phosphocellulose purified tubulin differed markedly between the different methods. MAPs isolated by addition of 0.35 M NaCl to taxol-stabilized microtubules stimul...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/BF00227754

    authors： Fridén B,Wallin M

    更新日期：1991-07-10 00:00:00

  • Long non-coding RNA HCG18 promotes M1 macrophage polarization through regulating the miR-146a/TRAF6 axis, facilitating the progression of diabetic peripheral neuropathy.

    abstract：:Diabetic peripheral neuropathy (DPN) is one of the most important complications in diabetes mellitus (DM), which has been reported to be modulated by long non-coding RNAs (lncRNAs). The purpose of the current study is to explore the regulatory mechanism of lncRNA HCG18 on DPN in vitro. The expression of lncRNA HCG18, ...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-020-03923-3

    authors： Ren W,Xi G,Li X,Zhao L,Yang K,Fan X,Gao L,Xu H,Guo J

    更新日期：2020-09-29 00:00:00

  • Reduced cardiac myofibrillar Mg-ATPase activity without changes in myosin isozymes in patients with end-stage heart failure.

    abstract：:In this study we tested the hypothesis that reduced myofibrillar ATPase activities in end-stage heart failure are associated with a redistribution of myosin isozymes. Cardiac myofibrils were isolated from left ventricular free wall from normal human hearts and hearts at end-stage heart failure caused by coronary arter...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/BF00228455

    authors： Alousi AA,Grant AM,Etzler JR,Cofer BR,Van der Bel-Kahn J,Melvin D

    更新日期：1990-07-17 00:00:00

  • Sequence-dependent conformations of short polypeptides in a hydrophobic environment.

    abstract：:Surfactants, which provide a hydrophobic environment, may induce an ordered conformation in polypeptides and proteins that contain a sequence with helix-or beta-forming potential. This hypothesis has been illustrated in circular dichroic studies of oligopeptides and short polypeptides. These peptide-surfactant complex...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/BF00224754

    authors： Wu CS,Yang JT

    更新日期：1981-10-30 00:00:00

  • The role of thrombospondin-1-mediated TGF-β1 on collagen type III synthesis induced by high glucose.

    abstract：:Transforming growth factor-β1 (TGF-β1) has been thought to play a major role during cardiac fibrosis in the development of diabetic cardiomyopathy, and cardiac fibrosis mainly as a result of an increase of collagen type III occurs in the human hearts with diabetes. Thrombospondin-1 (TSP-1) has been reported to activat...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-010-0590-7

    authors： Tang M,Zhou F,Zhang W,Guo Z,Shang Y,Lu H,Lu R,Zhang Y,Chen Y,Zhong M

    更新日期：2011-01-01 00:00:00

  • JIP3 localises to exocytic vesicles and focal adhesions in the growth cones of differentiated PC12 cells.

    abstract：:The JNK-interacting protein 3 (JIP3) is a molecular scaffold, expressed predominantly in neurons, that serves to coordinate the activation of the c-Jun N-terminal kinase (JNK) by binding to JNK and the upstream kinases involved in its activation. The JNK pathway is involved in the regulation of many cellular processes...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-017-3222-7

    authors： Caswell PT,Dickens M

    更新日期：2018-07-01 00:00:00

  • Magnetite induces oxidative stress and apoptosis in lung epithelial cells.

    abstract：:There is an ongoing concern regarding the biocompatibility of nanoparticles with sizes less than 100 nm as compared to larger particles of the same nominal substance. In this study, we investigated the toxic properties of magnetite stabilized with polyacrylate sodium. The magnetite was characterized by X-ray powder di...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-011-1174-x

    authors： Ramesh V,Ravichandran P,Copeland CL,Gopikrishnan R,Biradar S,Goornavar V,Ramesh GT,Hall JC

    更新日期：2012-04-01 00:00:00

  • Poly(I:C) induce bone marrow precursor cells into myeloid-derived suppressor cells.

    abstract：:Dendritic cells (DC) and myeloid-derived suppressor cells (MDSC) are important cells involved in immune response. DC can be generated from mouse bone marrow (BM) in the presence of granulocyte-macrophage colony-stimulating factor (GM-CSF) and IL-4. Recent studies have revealed that combined treatment of bone marrow MD...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-011-0982-3

    authors： Liu C,Zhang C,Lu H,Cai J,Wang Z,Chen J,Liu F,Wu Z,Liu X,Sun W

    更新日期：2011-12-01 00:00:00

  • Evaluation of antioxidant activity of epigallocatechin gallate in biphasic model systems in vitro.

    abstract：:The antioxidant activity of epigallocatechin gallate (EGCG) was studied in different in vitro model systems, which enabled evaluation of both chemical and physical factors involved in assessing the role of EGCG in oxidative reactions. EGCG suppressed the initiation rate and prolonged the lag phase duration of peroxyl ...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1023/a:1007220928446

    authors： Hu C,Kitts DD

    更新日期：2001-02-01 00:00:00

  • Effect of sex and bezafibrate on incorporation of blood borne palmitate into lipids of rat liver nuclei.

    abstract：:The aim of the present study was to investigate whether lipid metabolism in the nuclei is affected by changes in the metabolism of free fatty acids in the liver. The experiments were carried out on 3 groups of rats: 1 - control-male, 2 - female, and 3 - male, treated with bezafibrate (a peroxisome proliferator). The r...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1023/a:1007189503183

    authors： Górski J,Zendzian-Piotrowska M,Wolfrum C,Nawrocki A,Spener F

    更新日期：2000-11-01 00:00:00

  • Changes in fatty acid compositions of myocardial lipids in rats with heart failure following myocardial infarction.

    abstract：:Changes in fatty acid composition of myocardial lipids were examined in rats with heart failure following myocardial infarction. Left ventricular systolic pressure (LVSP) was decreased and left ventricular end-diastolic pressure (LVEDP) was elevated 24 h, 1 and 12 weeks after left coronary artery ligation (CAL), sugge...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：

    authors： Nasa Y,Sakamoto Y,Sanbe A,Sasaki H,Yamaguchi F,Takeo S

    更新日期：1997-11-01 00:00:00

  • Genomic organization and neonatal expression of the bovine myostatin gene.

    abstract：:Myostatin belongs to the Transforming Growth Factor-beta (TGF-beta) superfamily and is expressed in developing and mature skeletal muscle. Biologically, the role of myostatin seems to be extremely well conserved during evolution since inactivating mutations in myostatin gene cause similar phenotype of heavy muscling i...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1023/a:1010801511963

    authors： Jeanplong F,Sharma M,Somers WG,Bass JJ,Kambadur R

    更新日期：2001-04-01 00:00:00

  • Ionizing radiation induced signaling of DNA damage response molecules in RAW 264.7 and CD4⁺ T cells.

    abstract：:Ionizing radiation (IR) treatment results in activation of several DNA damage response molecules, such as ataxia telangiectasia, mutated (ATM), and DNA-dependent protein kinase (DNAPK) in mammals that are increasingly recognized for their potential roles in the sensing of DNA damage and initiating the subsequent prote...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-011-1156-z

    authors： Dhariwala FA,Narang H,Krishna M

    更新日期：2012-04-01 00:00:00

  • Spin trapping endogenous radicals in MC-1010 cells: evidence for hydroxyl radical and carbon-centered ascorbyl radical adducts.

    abstract：:Incubation of MC-1010 cells with the spin-trapping agent 5,5-dimethyl-1-pyrroline 1-oxide (DMPO) followed by brief treatment with the solid oxidant lead dioxide (PbO2) yielded, after filtration, a cell-free solution that contained two nitroxyl adducts. The first was the hydroxyl radical adduct, 5,5-dimethyl-2-hydroxyp...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/BF00928153

    authors： Bernofsky C,Bandara BM

    更新日期：1995-07-19 00:00:00

  • Cross-talk between NADPH oxidase-PKCα-p(38)MAPK and NF-κB-MT1MMP in activating proMMP-2 by ET-1 in pulmonary artery smooth muscle cells.

    abstract：:Treatment of bovine pulmonary artery smooth muscle cells with endothelin-1 (ET-1) caused an increase in the expression and activation of proMMP-2 in the cells. The present study was undertaken to determine the underlying mechanisms involved in this scenario. We demonstrated that (i) pretreatment with NADPH oxidase inh...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-016-2673-6

    authors： Sarkar J,Chowdhury A,Chakraborti T,Chakraborti S

    更新日期：2016-04-01 00:00:00