Effect of chronic colchicine administration on the myocardium of the aging spontaneously hypertensive rat.

abstract：:Thymol is a phytochemical component present in many plants used as food additive in order to promote animal growth due to its several biological properties. However, possible side effects of thymol remain poorly known limited to few reports. In this sense, we evaluated the enzymes of the purinergic signaling such as, ...

journal_title：Molecular and cellular biochemistry

authors： Baldissera MD,Souza CF,De Matos AFIM,Baldisserotto B,Stefani LM,da Silva AS

更新日期：2019-02-01 00:00:00

abstract：:Heat shock factor 1 (HSF1) is the major heat shock transcription factor and plays an essential role in mediating the cellular response to physiological and environmental stress. We found that LPS-induced expression of the granulocyte-colony stimulating factor (G-CSF) gene was upregulated in HSF1 knock-out (HSF1(-/-)) ...

journal_title：Molecular and cellular biochemistry

authors： Zhang L,Yang M,Wang Q,Liu M,Liang Q,Zhang H,Xiao X

更新日期：2011-06-01 00:00:00

abstract：:Long-chain fatty acids (e.g. arachidonic acid) have been implicated in physiological control of insulin secretion. We previously reported histidine phosphorylation of at least two islet proteins (e.g., NDP kinase and the beta subunit of trimeric G-proteins), and suggested that such a signalling step may have regulator...

journal_title：Molecular and cellular biochemistry

authors： Kowluru A

更新日期：2004-11-01 00:00:00

abstract：:Microtubule associated protein tau is considered to play roles in some types of human transmissible spongiform encephalopathies (TSE). In this study, the full-length and several truncated human tau proteins were expressed from E. coli and purified. Using GST pull down, co-immunoprecipitation assay and tau-coated ELISA...

journal_title：Molecular and cellular biochemistry

authors： Wang XF,Dong CF,Zhang J,Wan YZ,Li F,Huang YX,Han L,Shan B,Gao C,Han J,Dong XP

更新日期：2008-03-01 00:00:00

abstract：:Studies designed to examine effects of fat mass reduction (including lipodystrophy and lipectomy) on human serum total and LDL-cholesterol concentrations are inconsistent. The purpose of this study was to examine effect of partial lipectomy in rats (as an experimental model of fat mass reduction in humans) on (1) circ...

journal_title：Molecular and cellular biochemistry

authors： Dettlaff-Pokora A,Sucajtys-Szulc E,Sledzinski T

更新日期：2019-05-01 00:00:00

abstract：:NAD kinase (NADK, EC 2.7.1.23) is the sole NADP(+)-biosynthetic enzyme that catalyzes phosphorylation of NAD(+) to yield NADP(+) using ATP as a phosphoryl donor, and thus, plays a vital role in the cell and represents a potentially powerful antimicrobial drug target. Although methods for expression and purification of...

journal_title：Molecular and cellular biochemistry

authors： Ohashi K,Kawai S,Koshimizu M,Murata K

更新日期：2011-09-01 00:00:00

abstract：:The mainstream explanation of enzyme catalysis relies on the assumption that enzymes can utilize the binding energy. The author suggest that (i) an enzyme with excess free energy first gives a group from its active site into the final place of the bound reactant (substrate) in order to break the first initial chemical...

journal_title：Molecular and cellular biochemistry

authors： Foigel AG

更新日期：2011-06-01 00:00:00

abstract：:Photoreactive probes for the hydrophobic pocket of the liver fatty acid-binding protein, 11-(5'-azido-salicylamido)-undecanoic acid (5' ASU) and its acetyl ester (Ac5' ASU), were synthesized and their interaction with the protein was assessed. Fatty acid-binding proteins are closely related proteins which are abundant...

journal_title：Molecular and cellular biochemistry

authors： Atlasovich F,Santomé JA,Fernández HN

更新日期：1993-03-10 00:00:00

abstract：:Autoantibodies (AABs) against the second extracellular loop of the beta1-receptor (beta1(II)-AABs) are found as a pathogenic driver in patients with idiopathic dilated cardiomyopathy, Chagas cardiomyopathy, peripartum cardiomyopathy, and myocarditis, and have been increasingly seen as a treatment target. We recently i...

journal_title：Molecular and cellular biochemistry

authors： Haberland A,Wallukat G,Berg S,Schulz AM,Freyse EJ,Vetter R,Salzsieder E,Müller J,Kreutz R,Schimke I

更新日期：2014-08-01 00:00:00

abstract：:Total cellular creatine content is an important bioenergetic parameter in skeletal muscle. To understand its regulation we investigated creatine transport and accumulation in the G8 cultured skeletal myoblast line. Like other cell types, these contain a creatine transporter, whose activity, measured using a radiolabel...

journal_title：Molecular and cellular biochemistry

authors： Odoom JE,Kemp GJ,Radda GK

更新日期：1996-05-24 00:00:00

abstract：:The mitochondrial pathologies are a heterogeneous group of metabolic disorders that are characterized by anomalies of oxidative phosphorylation, especially in the respiratory chain. The diagnosis of these pathologies involves many investigations among which biochemical study is at present the main tool. However, the a...

journal_title：Molecular and cellular biochemistry

authors： Durrieu G,Letellier T,Antoch J,Deshouillers JM,Malgat M,Mazat JP

更新日期：1997-09-01 00:00:00

abstract：:Sertoli and peritubular myoid cells, the somatic cells of the seminiferous tubule, support growth and differentiation of developing germ cells. This action strictly depends on the availability of in situ synthesized retinoic acid and we have previously documented the ability of Sertoli, but not peritubular cell extrac...

journal_title：Molecular and cellular biochemistry

authors： Cavazzini D,Catizone A,Galdieri M,Ottonello S

更新日期：2003-10-01 00:00:00

abstract：:To evaluate the effects of the in vivo endotoxin treatment of the rat on (1) the contractile responses in the subsequently isolated papillary muscle to adrenergic and cholinergic agonists and (2) the biochemical parameters (cyclic GMP, nitric oxide synthesis, protein phosphorylation and ADP-ribosyslation) in the subse...

journal_title：Molecular and cellular biochemistry

authors： Sulakhe PV,Sandirasegarane L,Davis JP,Vo XT,Costain WJ,Mainra RR

更新日期：1996-10-01 00:00:00

abstract：:The aim of our work was to study (1) the antioxidant properties of lipoic acid (LA) and its reduced metabolite dihydrolipoic acid (DHLA) formed by reduction of LA and (2) the effects of treatment with LA and DHLA on (a) K(+) efflux from human red blood cells and (b) post-ischemic recovery and oxidative stress in isola...

journal_title：Molecular and cellular biochemistry

authors： Ghibu S,Lauzier B,Delemasure S,Amoureux S,Sicard P,Vergely C,Muresan A,Mogosan C,Rochette L

更新日期：2009-01-01 00:00:00

abstract：:Lipoprotein(a) [Lp(a)] is a highly atherogenic lipoprotein, whose metabolism is poorly understood. Efficient and secure drugs that can lower elevated plasma Lp(a) concentrations are currently lacking. Fibroblast growth factor-21 (FGF-21), a member of the FGFS super family, regulates glucose and lipid metabolism in hep...

journal_title：Molecular and cellular biochemistry

authors： Lin X,Li G,He X,Ma X,Zhang K,Zhang H,Zeng G,Wang Z

更新日期：2014-08-01 00:00:00

abstract：:In this study we tested the hypothesis that reduced myofibrillar ATPase activities in end-stage heart failure are associated with a redistribution of myosin isozymes. Cardiac myofibrils were isolated from left ventricular free wall from normal human hearts and hearts at end-stage heart failure caused by coronary arter...

journal_title：Molecular and cellular biochemistry

authors： Alousi AA,Grant AM,Etzler JR,Cofer BR,Van der Bel-Kahn J,Melvin D

更新日期：1990-07-17 00:00:00

abstract：:In order to initiate the study of the functional differences between myosin alkali light chain isoforms and to investigate the mechanisms of their differential expression, we have isolated cDNA clones for two human alkali light chain isoforms. Here we report DNA sequence and RNA blotting analyses that demonstrate that...

journal_title：Molecular and cellular biochemistry

authors： Wade R,Feldman D,Gunning P,Kedes L

更新日期：1989-06-01 00:00:00

abstract：:Angiotensin II (Ang II) has been shown to induce receptor activator of nuclear factor-κB ligand (RANKL) expression in osteoblasts associated with its effect on reactive oxygen species (ROS) production. The objective of the present study was to investigate the potential pathways by which Ang II induces RANKL expression...

journal_title：Molecular and cellular biochemistry

authors： Zhang Y,Zhang Y,Kou J,Wang C,Wang K

更新日期：2014-11-01 00:00:00

abstract：:The binding of nuclear factor on the promoter region of the regucalcin gene and the expression of regucalcin in the kidney cortex of rats was investigated. Nuclear extracts from kidney cortex were used for oligonucleotide competition gel mobility shift assay. An oligonucleotide between position -523 and -506 in the 5'...

journal_title：Molecular and cellular biochemistry

authors： Misawa H,Yamaguchi M

更新日期：2001-03-01 00:00:00

abstract：:A homeostatic balance exists between the cellular generation of oxidant species and endogenous antioxidants under normal physiological conditions. Human Immunodeficiency Virus (HIV) infection is known to affect this balance causing oxidative stress. However, the interaction of HIV infection with a substance abuse on c...

journal_title：Molecular and cellular biochemistry

authors： Pérez-Casanova A,Husain K,Noel RJ Jr,Rivera-Amill V,Kumar A

更新日期：2008-01-01 00:00:00

abstract：:Hepatic stellate cells (HSCs), also referred to as Ito cells, perisinusiodal cells and fat-storing cells, have numerous vital functions. They are the main extracellular matrix-producing cells within the liver and are involved in the storage of retinol. HSCs are also known to secrete a number of liver mitogens. Current...

journal_title：Molecular and cellular biochemistry

authors： Riccalton-Banks L,Bhandari R,Fry J,Shakesheff KM

更新日期：2003-06-01 00:00:00

abstract：:MicroRNAs have been implicated in regulating diverse cellular pathways. Emerging evidence indicates that miR-143 plays causal roles in cancer tumorigenesis as a tumor suppress gene; however, its role in prostate cancer tumorigenesis remains largely unknown. The aims of this study were to verify the effect of miR-143 o...

journal_title：Molecular and cellular biochemistry

authors： Xu B,Niu X,Zhang X,Tao J,Wu D,Wang Z,Li P,Zhang W,Wu H,Feng N,Wang Z,Hua L,Wang X

更新日期：2011-04-01 00:00:00

abstract：:Five isoforms of CK2 may exist simultaneously in yeast cells: free catalytic subunits CK2α', CK2α and three holoenzymatic structures composed of αα'ββ', α(2)ββ' and α'(2)ββ'. Each isolated and purified form exhibits properties typical for CK2, but they differ in substrate specificity as well as in sensitivity to speci...

journal_title：Molecular and cellular biochemistry

authors： Janeczko M,Masłyk M,Szyszka R,Baier A

更新日期：2011-10-01 00:00:00

abstract：:The RNase hydrolysis of random-coil (alkaline form) poly A follows biphasic kinetics at low salt concentrations. However, its resistance to RNase increases with the ionic strength. Helical (acidic form) poly A is alos susceptible to RNase but its hydrolysis follows first-order kinetics, and its resistance increases as...

journal_title：Molecular and cellular biochemistry

authors： Iqbal ZM

更新日期：1975-10-31 00:00:00

abstract：:Knowledge of transcription and translation has advanced our understanding of cardiac diseases. Here, we present the hypothesis that the stability of mRNA mediated by the 3'-untranslated region (3'-UTR) plays a role in changing gene expression in cardiovascular pathophysiology. Several proteins that bind to sequences i...

journal_title：Molecular and cellular biochemistry

authors： Misquitta CM,Iyer VR,Werstiuk ES,Grover AK

更新日期：2001-08-01 00:00:00

abstract：:Endocrine disruptors have become a global social and public health problem since the late 1980s. Bisphenol A (BPA) has a steroid-like skeleton similar to estrogen and progesterone, and is an endocrine disruptor that disturbs the physiological hormone balance. The potential involvement of BPA in malignancy of endometri...

journal_title：Molecular and cellular biochemistry

authors： Yaguchi T

更新日期：2019-02-01 00:00:00

abstract：:The involvement of a hypocalcemic hormone calcitonin (CT) in the expression of hepatic Ca(2+)-binding protein regucalcin mRNA was investigated. The change of regucalcin mRNA levels was analyzed by Northern blotting using liver regucalcin complementary DNA (0.9 kb). A single oral administration of calcium chloride (100...

journal_title：Molecular and cellular biochemistry

authors： Yamaguchi M,Kanayama Y,Shimokawa N

更新日期：1994-07-13 00:00:00

abstract：:Coupling of ATP-generating with ATP-consuming processes is an essential component in the cardiac bioenergetics responsible for optimal myocardial function. Although a number of enzymatic systems have been implicated in securing proper intracellular energy communication, their integrative response in a failing myocardi...

journal_title：Molecular and cellular biochemistry

authors： Dzeja PP,Pucar D,Redfield MM,Burnett JC,Terzic A

更新日期：1999-11-01 00:00:00

abstract：:Lens epithelial cells are the metabolic unit of the lens and antioxidant enzymes are mainly concentrated here. The purpose of this study was to maintain human lens epithelial cells (HLEC) in culture and examine the status of antioxidant enzymes (glutathione peroxidase (GSHPx), catalase (CAT), glutathione-S-transferase...

journal_title：Molecular and cellular biochemistry

authors： Mohanty I,Joshi S,Trivedi D,Srivastava S,Tandon R,Gupta SK

更新日期：2002-09-01 00:00:00

abstract：:This article summarizes recent ultrastructure findings from our laboratory and documents some of the information accumulated primarily since 1975 from many laboratories. Special attention is given to documentation by scanning electron microscopy which affords insight into platelet activation (adhesion, aggregation, re...

journal_title：Molecular and cellular biochemistry

authors： Barnhart MI

更新日期：1978-12-22 00:00:00