Abstract：
:Hepatic stellate cells (HSCs), also referred to as Ito cells, perisinusiodal cells and fat-storing cells, have numerous vital functions. They are the main extracellular matrix-producing cells within the liver and are involved in the storage of retinol. HSCs are also known to secrete a number of liver mitogens. Current isolation techniques are cumbersome and most require a pronase digestion step, which destroys any hepatocytes present. We present a simple method for isolation and culture of hepatic stellate cells from the normally discarded washings from a two-step collagenase hepatocyte isolation, which has shown a yield of more than 1.5 x 10(6) viable HSCs after 5 days in culture. The cells were positively identified as HSCs by staining for two intermediate filaments (desmin and GFAP) and observing their distinct morphology from other liver cell types. This efficient method allows rapid and consistent isolation of stellate cells to give a culture that may be passaged several times.
journal_name
Mol Cell Biochemjournal_title
Molecular and cellular biochemistryauthors
Riccalton-Banks L,Bhandari R,Fry J,Shakesheff KMdoi
10.1023/a:1024184826728keywords:
subject
Has Abstractpub_date
2003-06-01 00:00:00pages
97-102issue
1-2eissn
0300-8177issn
1573-4919journal_volume
248pub_type
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