当前位置： SCI文献检索 > MOLECULAR AND CELLULAR BIOCHEMISTRY期刊下所有文献 > Functional validation of ATF4 and GADD34 in Neuro2a cells by CRISPR/Cas9-mediated genome editing.

Functional validation of ATF4 and GADD34 in Neuro2a cells by CRISPR/Cas9-mediated genome editing.

Abstract：

:Activating transcription factor 4 (ATF4), which is ubiquitously expressed, plays a crucial role in regulating various stress-responsive genes under pathophysiological conditions. Further, growth arrest and DNA damage-inducible gene 34 (GADD34), a downstream target of ATF4, has been reported to negatively regulate ATF4 expression. To understand the relationship between intrinsic ATF4 and GADD34 under resting and ER stress conditions, we used a novel gene editing approach, CRISPR/Cas9, to integrate antibiotic-resistant genes into the target genes, ATF4 and GADD34. First, we manipulated the ATF4 gene in the mouse neuroblastoma cell line, Neuro2a, and compared the ER stress responses between parental and ATF4-edited Neuro2a cells. Next, we established Neuro2a cells with edited GADD34 and ATF4/GADD34 genes and found that ATF4 acts as a proapoptotic factor, but GADD34 depletion did not attenuate the expression of cleaved caspase-3 induced by tunicamycin treatment. These findings provide new insights into the ATF4 signaling cascades. Additionally, the rapid establishment of cells lacking multiple genes using this CRISPR/Cas9 system will be a powerful tool for exploring various cellular issues under pathophysiological conditions.

journal_name

Mol Cell Biochem

journal_title

Molecular and cellular biochemistry

authors

Oh-Hashi K,Sugiura N,Amaya F,Isobe KI,Hirata Y

doi

10.1007/s11010-017-3156-0

subject

Has Abstract

pub_date

2018-03-01 00:00:00

pages

65-75

issue

1-2

eissn

0300-8177

issn

1573-4919

pii

10.1007/s11010-017-3156-0

journal_volume

440

pub_type

杂志文章

相关文献

MOLECULAR AND CELLULAR BIOCHEMISTRY文献大全
  • Effect of sinomenine on vascular smooth muscle cell dedifferentiation and neointima formation after vascular injury in mice.

    abstract：:Sinomenine, a pure alkaloid extract from Sinomenium acutum, has anti-inflammatory and immunoregulatory functions. This study investigated the efficiency and the signalling pathways involved in the effect of sinomenine on vascular smooth muscle cell (VSMC) dedifferentiation in response to platelet-derived growth factor...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-012-1474-9

    authors： Zhu L,Hao Y,Guan H,Cui C,Tian S,Yang D,Wang X,Zhang S,Wang L,Jiang H

    更新日期：2013-01-01 00:00:00

  • PKCepsilon inhibits the hyperglycemia-induced apoptosis signal in adult rat ventricular myocytes.

    abstract：:Recruitment of the protein kinase C (PKC) family of isozymes is an integral component of the signaling events that direct cardiac phenotype expressed during postnatal development and in response to pathologic stimuli. Hyperglycemia is a potent activating signal for cardiac PKC isozymes and induces the apoptosis progra...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-005-3858-6

    authors： Malhotra A,Kang BP,Hashmi S,Meggs LG

    更新日期：2005-01-01 00:00:00

  • Hypoglycemic and antihyperglycemic effect of Brassicajuncea diet and their effect on hepatic glycogen content and the key enzymes of carbohydrate metabolism.

    abstract：:Plants in the form of food or other remedial forms have been used for the treatment of various human ailments. Diabetes is one such disorder in which various form of plants and herbal remedies have been tried. Brassicajuncea (BJ) seeds (Rai) are consumed in India as a spice in various food items. Previous studies have...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1023/a:1020814709118

    authors： Grover JK,Yadav S,Vats V

    更新日期：2002-12-01 00:00:00

  • Human intestine epithelial cell acetyl- and butyrylcholinesterase.

    abstract：:The epithelial cells of the human intestine exhibit a cholinesterase activity which is restricted to the apex of the villi. This activity displays a maximum in the colon and a minimum in the jejunum. Contrary to most of the studied vertebrates, the human cells present both acetylcholinesterase and butyrylcholinesteras...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/BF00233119

    authors： Sine JP,Ferrand R,Cloarec D,Lehur PA,Colas B

    更新日期：1991-12-11 00:00:00

  • Vitamin E-deficiency induced changes in ovary and uterus.

    abstract：:Female rats at weaning (30 days age) were maintained on vitamin E-deficient diet for 70 days. The vitamin E-deficient and control animals were sacrificed on 100 days of age. To study recovery a group of animals were supplemented with normal diet for last 25 days after initial 45 days of deficient diet or vice versa. T...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1023/a:1006954032164

    authors： Das P,Chowdhury M

    更新日期：1999-08-01 00:00:00

  • Cannabidiol promotes browning in 3T3-L1 adipocytes.

    abstract：:Recruitment of the brown-like phenotype in white adipocytes (browning) and activation of existing brown adipocytes are currently being investigated as a means to combat obesity. Thus, a wide variety of dietary agents that contribute to browning of white adipocytes have been identified. The present study was designed t...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-016-2702-5

    authors： Parray HA,Yun JW

    更新日期：2016-05-01 00:00:00

  • Enzymes of carbohydrate metabolism in young and adult rats fed diets differing in fat and carbohydrate.

    abstract：:Glycogen content as well as glycolytic, gluconeogenic and fatty acid synthesis enzyme activities were monitored in young and adult male rats fed diets differing in fat content: 11% (low), 22% (medium) and 42% (high) of total energy from fat. The results showed significant differences in the responses of young and adul...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/BF00226063

    authors： Brooks SP,Lampi BJ

    更新日期：1996-06-07 00:00:00

  • Binding of glyceraldehyde 3-phosphate dehydrogenase to microtubules.

    abstract：:The interaction of glyceraldehyde 3-phosphate dehydrogenase with microtubules has been studied by measurement of the amount of enzyme which co-assembles with in vitro reconstituted microtubules. The binding of glyceraldehyde 3-phosphate dehydrogenase to microtubules is a saturable process; the maximum binding capacity...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/BF00221912

    authors： Durrieu C,Bernier-Valentin F,Rousset B

    更新日期：1987-03-01 00:00:00

  • Role of H2O2 in changing beta-adrenoceptor and adenylyl cyclase in ischemia-reperfused hearts.

    abstract：:In view of the accumulation of H2O2 in the myocardium due to ischemia-reperfusion and changes in beta-adrenoceptor mechanisms in the ischemic-reperfused heart, we investigated the effects of H2O2 on the beta-adrenoceptor, G-protein and adenylyl cyclase complex. Rat hearts were perfused with 1 mM H2O2 for 10 min before...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：

    authors： Persad S,Panagia V,Dhalla NS

    更新日期：1998-09-01 00:00:00

  • Spin trapping endogenous radicals in MC-1010 cells: evidence for hydroxyl radical and carbon-centered ascorbyl radical adducts.

    abstract：:Incubation of MC-1010 cells with the spin-trapping agent 5,5-dimethyl-1-pyrroline 1-oxide (DMPO) followed by brief treatment with the solid oxidant lead dioxide (PbO2) yielded, after filtration, a cell-free solution that contained two nitroxyl adducts. The first was the hydroxyl radical adduct, 5,5-dimethyl-2-hydroxyp...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/BF00928153

    authors： Bernofsky C,Bandara BM

    更新日期：1995-07-19 00:00:00

  • MiR-96 downregulates RECK to promote growth and motility of non-small cell lung cancer cells.

    abstract：:MicroRNAs play critical roles in the development and progression of non-small cell lung cancer (NSCLC). miR-96 acts as an oncogene in some malignancies, while its role in NSCLC is unclear. Here, we validated that miR-96 was significantly increased in both human NSCLC tissues and cell lines. Inhibition of miR-96 expres...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-014-1966-x

    authors： Guo H,Li Q,Li W,Zheng T,Zhao S,Liu Z

    更新日期：2014-05-01 00:00:00

  • Modulation of the c-Jun N-terminal kinase activity in the embryonic heart in response to anoxia-reoxygenation: involvement of the Ca2+ and mitoKATP channels.

    abstract：:Whether the response of the fetal heart to ischemia-reperfusion is associated with activation of the c-Jun N-terminal kinase (JNK) pathway is not known. In contrast, involvement of the sarcolemmal L-type Ca2+ channel (LCC) and the mitochondrial KATP (mitoKATP) channel has been established. This work aimed at investiga...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-008-9750-4

    authors： Sarre A,Gardier S,Maurer F,Bonny C,Raddatz E

    更新日期：2008-06-01 00:00:00

  • HMGb1 promotes scratch wound closure of HaCaT keratinocytes via ERK1/2 activation.

    abstract：:HMGb1 is a DNA-binding protein whose role as an extracellular cytokine in inflammation and tissue regeneration has also been reported. Given the importance of keratinocytes in wound healing, we have studied the mechanism of action of HMGb1 on HaCaT keratinocytes during in vitro scratch wound repair. Western blot and c...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-009-0192-4

    authors： Ranzato E,Patrone M,Pedrazzi M,Burlando B

    更新日期：2009-12-01 00:00:00

  • Activation of the integrins alpha 5beta 1 and alpha v beta 3 and focal adhesion kinase (FAK) during arteriogenesis.

    abstract：:Migration and proliferation of smooth muscle cells (SMC) are important events during arteriogenesis, but the underlying mechanism is still only partially understood. The present study investigates the expression of integrins alpha 5 beta 1 and v beta 3 as well as focal adhesion kinase (FAK) and phosphorylated FAK (pY3...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-008-9953-8

    authors： Cai WJ,Li MB,Wu X,Wu S,Zhu W,Chen D,Luo M,Eitenmüller I,Kampmann A,Schaper J,Schaper W

    更新日期：2009-02-01 00:00:00

  • Alterations in the activities of rat tissue hexose monophosphate dehydrogenases in response to premature weaning and dietary restriction at mid-lactation.

    abstract：:The activities of the hexose monophosphate dehydrogenases increased in adipose tissue, remained unchanged in liver and decreased in mammary gland following the weaning of rats at mid-lactation (day 14). When dietary intake was restricted at mid-lactation, the activities of the hexose monophosphate dehydrogenases incre...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/BF00223478

    authors： Martins RN,Stokes GB

    更新日期：1987-08-01 00:00:00

  • The abnormal expressions of tristetraprolin and the VEGF family in uraemic rats with peritoneal dialysis.

    abstract：:The effect of peritoneal dialysis (PD) with high-glucose dialysis fluid on the VEGF family, tristetraprolin (TTP), angiogenesis and lymphangiogenesis was investigated. Forty male SD rats were randomised into five groups: normal group, sham operation group, uraemia group, PD 2-week group and PD4-week group. After 4 wee...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-014-2033-3

    authors： Xiao J,Gao H,Jin Y,Zhao Z,Guo J,Liu Z,Zhao Z

    更新日期：2014-07-01 00:00:00

  • Membrane bound and soluble adenosine triphosphatase of Escherichia coli K 12. Kinetic properties of the basal and trypsin-stimulated activities.

    abstract：:Basal and trypsin-stimulated adenosine triphosphatase activities of Escherichia coli K 12 have been characterized at pH 7.5 in the membrane-bound state and in a soluble form of the enzyme. The saturation curve for Mg2+/ATP = 1/2 was hyperbolic with the membrane-bound enzyme and sigmoidal with the soluble enzyme. Tryps...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/BF01732200

    authors： Carreira J,Muñoz E

    更新日期：1975-11-14 00:00:00

  • The sole serine/threonine protein kinase and its cognate phosphatase from Aquifex aeolicus targets pyrimidine biosynthesis.

    abstract：:Serine/Threonine kinases participate in complex, interacting signaling pathways in eukaryotes, prokaryotes, and archae. While most organisms contain many different kinases, the extreme hyperthermophile, Aquifex aeolicus encodes a single hypothetical Ser/Thr kinase. A gene homologous to eukaryotic protein phosphatases ...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-008-9710-z

    authors： Purcarea C,Fernando R,Evans HG,Evans DR

    更新日期：2008-04-01 00:00:00

  • Active transport of Ca2+ in bacteria: bioenergetics and function.

    abstract：:The bioenergetics of Ca2+ transport in bacteria are discussed with special emphasis on the interrelationship between transport and other cellular functions such as substrate oxidation by the respiratory chain and oxidative phosphorylation. The unusual polarity of Ca2+ movement provides an exceptional tool to compare a...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章,评审

    doi：10.1007/BF02354906

    authors： Devés R,Brodie AF

    更新日期：1981-04-27 00:00:00

  • The activities of antioxidant enzymes and the level of malondialdehyde in cerebellum of rats subjected to methotrexate: protective effect of caffeic acid phenethyl ester.

    abstract：:Methotrexate (MTX), a folic acid antagonist, is widely used as a cytotoxic chemotherapeutic agent. MTX-associated neurotoxicity is an important clinical problem. The aim of this study was to investigate the role of caffeic acid phenethyl ester (CAPE) on cerebellar oxidative stress induced by MTX in rats. A total of 19...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-006-9196-5

    authors： Uzar E,Koyuncuoglu HR,Uz E,Yilmaz HR,Kutluhan S,Kilbas S,Gultekin F

    更新日期：2006-10-01 00:00:00

  • In vitro cellular uptake and neuroprotective efficacy of poly-arginine-18 (R18) and poly-ornithine-18 (O18) peptides: critical role of arginine guanidinium head groups for neuroprotection.

    abstract：:We have previously demonstrated that Cationic Arginine-Rich Peptides (CARPs) and in particular poly-arginine-18 (R18; 18-mer of arginine) exhibit potent neuroprotective properties in both in vitro and in vivo neuronal injury models. Based on the current literature, there is a consensus that arginine residues by virtue...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-019-03646-0

    authors： MacDougall G,Anderton RS,Ouliel E,Gao J,Redmond SL,Knuckey NW,Meloni BP

    更新日期：2020-01-01 00:00:00

  • High-fructose diet is as detrimental as high-fat diet in the induction of insulin resistance and diabetes mediated by hepatic/pancreatic endoplasmic reticulum (ER) stress.

    abstract：:In the context of high human consumption of fructose diets, there is an imperative need to understand how dietary fructose intake influence cellular and molecular mechanisms and thereby affect β-cell dysfunction and insulin resistance. While evidence exists for a relationship between high-fat-induced insulin resistanc...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-016-2828-5

    authors： Balakumar M,Raji L,Prabhu D,Sathishkumar C,Prabu P,Mohan V,Balasubramanyam M

    更新日期：2016-12-01 00:00:00

  • Synergistic effect of TRM2/RNC1 and EXO1 in DNA double-strand break repair in Saccharomyces cerevisiae.

    abstract：:In our recently published study, we provided in vitro as well as in vivo data demonstrating the involvement of TRM2/RNC1 in homologous recombination based repair (HRR) of DNA double strand breaks (DSBs), in support of such claims reported earlier. To further validate its role in DNA DSB processing, our present study r...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-007-9493-7

    authors： Choudhury SA,Asefa B,Kauler P,Chow TY

    更新日期：2007-10-01 00:00:00

  • Cloning and characterization of the rat TIS11 gene.

    abstract：:The TIS11 gene is an immediate early gene that is induced rapidly and transiently by phorbol 12-myristate 13-acetate and various growth factors. To study transcriptional regulation of the gene, a genomic clone of rat TIS11 was isolated, and the organization of exon-intron structure and transcriptional initiation site ...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1023/a:1007172316657

    authors： Kaneda N,Murata T,Niimi Y,Minamiyama M

    更新日期：2000-10-01 00:00:00

  • Mitoapocynin, a mitochondria targeted derivative of apocynin induces mitochondrial ROS generation and apoptosis in multiple cell types including cardiac myoblasts: a potential constraint to its therapeutic use.

    abstract：:Mitoapocynin is a triphenylphosphonium conjugated derivative of apocynin that specifically locates to the mitochondria. It has been developed as a mitochondrially targeted therapeutic antioxidant. We attempted to attenuate the mitochondrial ROS induced in H9c2 cardiac myoblast cells treated with norepinephrine. Mitoap...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-020-04039-4

    authors： Mahmood A,Bisoyi P,Banerjee R,Yousuf M,Goswami SK

    更新日期：2021-01-30 00:00:00

  • Investigation of antioxidative effects of a cardioprotective solution in heart tissue.

    abstract：:A multi-component solution, containing α-ketoglutaric acid (α-KG), 5-hydroxymethylfurfural (5-HMF), N-acetyl-seleno-L-methionine (NASeLM), and N-acetyl-L-methionine (NALM) as active ingredients, has been tested considering its supposed antioxidative effect with respect to heart transplantations. Oxidative stress was i...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-019-03591-y

    authors： Russ M,Jauk S,Wintersteiger R,Andrä M,Brcic I,Ortner A

    更新日期：2019-11-01 00:00:00

  • 17β-Estradiol and/or estrogen receptor alpha blocks isoproterenol-induced calcium accumulation and hypertrophy via GSK3β/PP2A/NFAT3/ANP pathway.

    abstract：:The present study was aimed to investigate the protective effects of 17β-estradiol (E2) and estrogen receptor α (ERα) on isoproterenol (ISO)-treated H9c2 cardiomyoblast cells. In the present study, we treated H9c2 cells with ISO, a β-adrenergic receptor agonist, to induce myocardiac hypertrophy. Pre-administration of ...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-017-3048-3

    authors： Pai P,Velmurugan BK,Kuo CH,Yen CY,Ho TJ,Lin YM,Chen YF,Lai CH,Day CH,Huang CY

    更新日期：2017-10-01 00:00:00

  • Altered coupling states between calcium transport and (Ca2+, Mg2+)-ATPase in the AS-30D ascites hepatocarcinoma plasma membrane.

    abstract：:Plasma membrane fractions from normal, regenerating liver and the AS-30D ascites hepatocarcinoma exhibited a high degree of enrichment when a set of plasma membrane enzyme markers were studied in comparison to the ones associated to the mitochondrial and cytosolic compartments. While the (Ca2+, Mg2+)-ATPase observed f...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/BF00230808

    authors： Mas-Oliva J,Pérez-Montfort R,Cárdenas-García M,Rivas-Duro M

    更新日期：1991-01-16 00:00:00

  • Involvement of Annexin A2 in p53 induced apoptosis in lung cancer.

    abstract：:Tumor suppressor p53 plays important roles in cell cycle regulation, apoptosis and DNA repair in different cell types including lung cancer. There are different p53 apoptotic pathways in high and low metastatic ability lung cancer cells. However, the exactly mechanism in the pathway is still unclear. Here we found tha...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-007-9649-5

    authors： Huang Y,Jin Y,Yan CH,Yu Y,Bai J,Chen F,Zhao YZ,Fu SB

    更新日期：2008-02-01 00:00:00

  • Liver fatty acid binding protein gene ablation enhances age-dependent weight gain in male mice.

    abstract：:Although studies performed in vitro and with transfected cells in culture suggest a role for liver fatty acid binding protein (L-FABP) in regulating fatty acid oxidation and fat deposition, the physiological significance of this possibility is not completely clear. To begin to address this question, the effect of L-FA...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-008-9989-9

    authors： Martin GG,Atshaves BP,McIntosh AL,Payne HR,Mackie JT,Kier AB,Schroeder F

    更新日期：2009-04-01 00:00:00