Abstract：
:In view of the accumulation of H2O2 in the myocardium due to ischemia-reperfusion and changes in beta-adrenoceptor mechanisms in the ischemic-reperfused heart, we investigated the effects of H2O2 on the beta-adrenoceptor, G-protein and adenylyl cyclase complex. Rat hearts were perfused with 1 mM H2O2 for 10 min before isolating membranes for measuring the biochemical activities. The stimulation of adenylyl cyclase by different concentrations of isoproterenol was depressed upon perfusing hearts with H2O2. Both the affinity and density of beta1-adrenoceptors as well as the density of the beta2-adrenoceptors were decreased whereas the affinity of beta2-adrenoceptors was increased by H2O2 perfusion. Competition curves did not reveal any effect of H2O2 on the proportion of coupled receptors in the high affinity state. The basal as well as forskolin-, NaF- and Gpp(NH)p-stimulated adenylyl cyclase activities were depressed by perfusing the heart with H2O2. Catalase alone or in combination with mannitol was able to significantly decrease the magnitude of alterations due to H2O2. The positive inotropic effect of 1 microM isoproterenol was markedly attenuated upon perfusing hearts with 200-500 microM H2O2 for 10 min. These results suggest that H2O2 may depress the beta1-adrenoceptor, Gs-proteins and catalytic subunit of the adenylyl cyclase enzyme and thus may play an important role in attenuating the beta-adrenoceptor linked signal transduction due to ischemia-reperfusion injury.
journal_name
Mol Cell Biochemjournal_title
Molecular and cellular biochemistryauthors
Persad S,Panagia V,Dhalla NSsubject
Has Abstractpub_date
1998-09-01 00:00:00pages
99-106issue
1-2eissn
0300-8177issn
1573-4919journal_volume
186pub_type
杂志文章abstract：:The continuous advancements in cancer research have contributed to the overwhelming evidence of the presence of telomerase in primary and secondary tumours together with hsp90 and c-Myc. This review will discuss the important role of telomerase together with hsp90 and c-Myc within the initiation and progression of gli...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章,评审
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更新日期：2006-02-01 00:00:00
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
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更新日期：2005-08-01 00:00:00
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
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更新日期：1996-09-20 00:00:00
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
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更新日期：1990-03-27 00:00:00
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更新日期：2007-07-01 00:00:00
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更新日期：1994-06-15 00:00:00
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更新日期：2014-09-01 00:00:00
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更新日期：2008-01-01 00:00:00
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章,评审
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更新日期：2003-05-01 00:00:00
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journal_title：Molecular and cellular biochemistry
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doi：
更新日期：1998-07-01 00:00:00
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-021-04058-9
更新日期：2021-01-28 00:00:00
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
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更新日期：2009-11-01 00:00:00
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
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更新日期：2003-08-01 00:00:00
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
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更新日期：2004-06-01 00:00:00
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
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更新日期：1995-05-24 00:00:00
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更新日期：1992-12-16 00:00:00
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journal_title：Molecular and cellular biochemistry
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doi：
更新日期：2001-11-01 00:00:00
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更新日期：2015-08-01 00:00:00
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更新日期：2002-09-01 00:00:00
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更新日期：2010-11-01 00:00:00
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更新日期：2013-12-01 00:00:00
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journal_title：Molecular and cellular biochemistry
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doi：
更新日期：2001-06-01 00:00:00
abstract：:Apoptosis is a physiological mechanism for the control of DNA integrity in mammalian cells. Vanadium induces both DNA damage and apoptosis. It is suggested that vanadium-induced apoptosis serves to eliminate DNA-damaged cells. This study is designed to clarify a role of reactive oxygen species in the mechanism of apop...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
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更新日期：1999-12-01 00:00:00
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journal_title：Molecular and cellular biochemistry
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更新日期：2014-11-01 00:00:00
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journal_title：Molecular and cellular biochemistry
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更新日期：2001-05-01 00:00:00
abstract：:There is a striking interaction of genes and environment in the etiology of type 2 diabetes mellitus (T2DM). While endocrine disrupting chemicals (EDCs) like bisphenol-A (BPA) have received special attention for their mechanistic role in metabolic disruption, there is a lack of clinically relevant data on BPA levels i...
journal_title：Molecular and cellular biochemistry
pub_type： 临床试验,杂志文章
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更新日期：2019-08-01 00:00:00
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journal_title：Molecular and cellular biochemistry
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更新日期：2003-01-01 00:00:00
