Abstract：
:Experiments were designed to determine if cotton bract tannin, a component of cotton mill dust, would promote the phosphorylation of alveolar macrophage proteins in doses potentially achievable in vivo. Rabbit alveolar macrophages were loaded with 32PO4 and challenged with various doses of tannin for time periods ranging from two seconds to 120 minutes. Changes in protein phosphorylation began after two seconds and were maximal at five to fifteen minutes. Dose response studies using an exposure time of one hour showed phosphorylation changes began at 1 micrograms/mL and were maximal at 10 to 30 micrograms/mL. Phosphorylation changes were similar to but not identical to those induced by the protein kinase C activator, phorbol myristate acetate (PMA). Calcium ionophore, A-23187 had no clear effect either alone or in conjunction with PMA. These results indicate that cotton bract tannin is able to rapidly promote protein phosphorylation of alveolar macrophages at doses potentially achievable in vivo. Other mechanisms in addition to those of protein kinase C appear to be involved in this protein phosphorylation process.
journal_name
Mol Cell Biochemjournal_title
Molecular and cellular biochemistryauthors
Hempel SL,Rohrbach MSdoi
10.1007/BF00222616subject
Has Abstractpub_date
1989-04-11 00:00:00pages
163-70issue
2eissn
0300-8177issn
1573-4919journal_volume
86pub_type
杂志文章abstract：:The TP53 signal transduction pathway is an attractive target for cancer treatments. In this study, we conducted a comprehensive molecular evaluation of 907 patients with cancer in Japan to identify genomic alterations in the TP53 pathway. TP53 mutations were frequently detected in many cancers, except melanoma, thymic...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
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更新日期：2017-07-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s11010-017-3017-x
更新日期：2017-09-01 00:00:00
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-008-9710-z
更新日期：2008-04-01 00:00:00
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章,评审
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
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pub_type： 杂志文章,评审
doi：10.1007/BF00925964
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更新日期：2007-07-01 00:00:00
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
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更新日期：2007-10-01 00:00:00
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更新日期：2019-01-01 00:00:00
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/BF00223492
更新日期：1990-03-05 00:00:00
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1023/a:1006835808554
更新日期：1999-04-01 00:00:00
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pub_type： 杂志文章,评审
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更新日期：2002-08-01 00:00:00
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更新日期：1990-06-25 00:00:00
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更新日期：2016-01-01 00:00:00
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更新日期：1987-03-01 00:00:00
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journal_title：Molecular and cellular biochemistry
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更新日期：2000-09-01 00:00:00
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pub_type： 杂志文章
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更新日期：1988-12-01 00:00:00
