On the cell-free system of protein synthesis from mammalian mitochondria.

Abstract:

:Some properties of a submitochondrial cell-free system for protein synthesis are described. The system was prepared from rat liver mitochondria lysed with Triton X-100, and the lysate was characterized by a linear rate of [14C]amino acid incorporation for 15-20 min with subsequent decline in activity. The incorporation reaction was inhibited by chloramphenicol and was insensitive to cycloheximide. Poly(U) addition stimulated [14CA1phenylalanine incorporation by the preincubated submitochondrial system. Upon the addition of 7.5S mRNA that was isolated from mitochondria the major translation product was identified as a hydrophobic poly-peptide which in some properties (solubility in chloroform-methanol mixture) was similar to one of polypeptides synthesized by the sub-mitochondrial system on endogeneous mRNAs.

journal_name

Mol Cell Biochem

authors

Kisselev OI,Gaitskhoki VS,Neifakh SA

doi

10.1007/BF01734170

subject

Has Abstract

pub_date

1977-02-04 00:00:00

pages

91-6

issue

1-3

eissn

0300-8177

issn

1573-4919

journal_volume

14

pub_type

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