当前位置： SCI文献检索 > MOLECULAR AND CELLULAR BIOCHEMISTRY期刊下所有文献 > Molecular cloning and expression of alpha2,8-sialyltransferase (ST8Sia I, GD3 Synthase) in Xenopus.

Molecular cloning and expression of alpha2,8-sialyltransferase (ST8Sia I, GD3 Synthase) in Xenopus.

Abstract：

:GD3, a minor ganglioside in most normal tissues, is involved in important biological events and its expression could increase in pathological conditions. Organism integrity requires a tight balance between the anabolic and catabolic processes, thus it is important to control the intracellular expression of those "key" enzymes, which act at the "branching point" of ganglioside metabolism; one of these is the GD3-synthase (ST8Sia I). In this paper, we report the sequences of two ST8Sia I mRNAs found in Xenopus laevis and their genomic organization; the canonical form resulted constituted of 5 exons and 4 introns, while the "short" mRNA lacks of the exon 2. The expression of the two ST8Sia I mRNAs during embryo development and their tissue distribution in adult animals showed the single or simultaneous presence of the two forms. Experiments of in vitro expression and evaluation of enzymatic activity of the two hypothetical proteins turned out to be ST8Sia I. In the end, considering the growing interest toward the specie Xenopus tropicalis, due to its diploid genome that render it more suitable for genetic studies, we also cloned X. tropicalis ST8Sia I.

journal_name

Mol Cell Biochem

journal_title

Molecular and cellular biochemistry

authors

Rimoldi S,Papis E,Bernardini G,Prati M,Gornati R

doi

10.1007/s11010-006-9406-1

subject

Has Abstract

pub_date

2007-07-01 00:00:00

pages

143-53

issue

1-2

eissn

0300-8177

issn

1573-4919

journal_volume

301

pub_type

杂志文章

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