Abstract：
:We investigated retinol effects in ornithine decarboxylase activity in Sertoli cells. We also tested the hypothesis that free radical scavengers and iron chelators may attenuate the effect of retinol. Sertoli cells isolated from 15-day-old Wistar rats were previously cultured for 48 h and then treated with retinol by 24 h with or without mannitol (1 mM) or 1,10 phenanthroline (100 microM). We measured ornithine decarboxylase and catalase activities and malondialdehyde concentrations in response to retinol treatment. In response to 7 microM retinol treatment ornithine decarboxylase activity increased 30%. Retinol-induced ornithine decarboxylase activity was significantly decreased by addition of free radical scavenger (mannitol) or iron chelator (1,10 phenanthroline). In addition the same effect was observed in catalase increased activity and in malondialdehyde concentrations. These results suggest that retinol treatment induced ornithine decarboxylase and catalase activity and increased malondialdehyde concentration. These effects appear to be mediate by ROS.
journal_name
Mol Cell Biochemjournal_title
Molecular and cellular biochemistryauthors
Klamt F,Dal-Pizzol F,Ribeiro NC,Bernard EA,Benfato MS,Moreira JCdoi
10.1023/a:1007081703928keywords:
subject
Has Abstractpub_date
2000-05-01 00:00:00pages
71-6issue
1-2eissn
0300-8177issn
1573-4919journal_volume
208pub_type
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