Abstract:
:Bladder smooth muscle contraction is mediated by both direct calcium entry through the cell membrane, and by calcium induced calcium release (CICR) from the sarcoplasmic reticulum (SR) storage sites. Ryanodine is a neutral plant alkaloid which binds to an ion channel located on the SR membrane. Its effects in cardiac skeletal muscle are well characterized where it inhibits the efflux of intracellular calcium stores, and thus it serves as a negative inotrope. It has also been shown that in the developing rabbit myocardium, there is a gradual increase in the expression of this ion channel. Little has been written about the expression and function of the ryanodine sensitive ion channel in smooth muscle. Recently we have shown that neonatal rabbit bladder smooth muscle is not very sensitive to ryanodine, while that from mature rabbits is extremely sensitive. This leads us to quantify the expression of the ryanodine sensitive ion channel. In this paper we demonstrate that the Kd values do not change to any significant degree with normal rabbit bladder development. However the Bmax values for 3 day, 2, 4, 6, and 8 week rabbit bladder smooth muscle are 7, 10, 15, 29, and 44 fmol specifically bound ryanodine/mg protein. The differences between the neonatal groups and the mature groups are significant (P < 0.5). This increase in ryanodine sensitive ion channel expression with normal growth would suggest that with normal maturation, the bladder smooth muscle cell acquires an increased pool of sequestered intracellular calcium. This would follow a similar pattern of development that has already been described in rabbit myocardium.
journal_name
Mol Cell Biochemjournal_title
Molecular and cellular biochemistryauthors
Gong C,Zderic SA,Levin RMdoi
10.1007/BF00944078subject
Has Abstractpub_date
1994-08-31 00:00:00pages
169-72issue
2eissn
0300-8177issn
1573-4919journal_volume
137pub_type
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