Abstract:
:Pancreatic islet cytosol contains a calcium-calmodulin dependent protein kinase that can mediate the phosphorylation of an endogenous protein that has an Mr of 57 000, as well as exogenous muscle pyruvate kinase (subunit Mr, 57 000). EGTA and trifluoperazine decreased the phosphorylation. Alkaline inactivation of pyruvate kinase made it a better substrate for the kinase. As in rat islet cytosol, rabbit islet cytosol catalyzed the phosphorylation of a 57 000 Mr protein in the presence of calcium and calmodulin. This phosphoprotein was immunoprecipitated with anti-pyruvate kinase antibody. This is consistent with the idea that the 57 000 Mr phosphoprotein in islet cytosol is the subunit of pyruvate kinase. The paper following this paper shows that the kinetic and immunologic properties of the islet pyruvate kinase indicate it is the M2 isoenzyme and that its phosphorylation does not affect its catalytic activity.
journal_name
Mol Cell Biochemjournal_title
Molecular and cellular biochemistryauthors
MacDonald MJ,Kowluru Adoi
10.1007/BF00219374subject
Has Abstractpub_date
1985-10-01 00:00:00pages
107-14issue
2eissn
0300-8177issn
1573-4919journal_volume
68pub_type
杂志文章abstract::Sirtuin 3 (SIRT3) modulates mitochondria-localized processes and is implicated in the metabolic reprogramming of cancer cells, especially fatty acid (FA) synthesis. However, the relationship between SIRT3 and aberrant lipid synthesis in cervical cancer remains unclear. Here, we investigated the clinical relevance of S...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/s11010-019-03644-2
更新日期:2020-01-01 00:00:00
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journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/BF00224754
更新日期:1981-10-30 00:00:00
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journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/s11010-007-9585-4
更新日期:2008-01-01 00:00:00
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journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/s11010-008-9816-3
更新日期:2008-09-01 00:00:00
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journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/BF02354825
更新日期:1981-03-13 00:00:00
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pub_type: 杂志文章
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journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
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journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
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更新日期:1990-06-25 00:00:00
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pub_type: 杂志文章
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journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
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journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/BF01075920
更新日期:1995-12-06 00:00:00
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journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/s11010-019-03591-y
更新日期:2019-11-01 00:00:00
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pub_type: 杂志文章
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更新日期:2010-05-01 00:00:00
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pub_type: 杂志文章
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journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/BF02354886
更新日期:1981-07-01 00:00:00
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journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/s11010-017-2961-9
更新日期:2017-06-01 00:00:00
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journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章,评审
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更新日期:1996-04-12 00:00:00
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journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/s11010-005-1082-z
更新日期:2005-07-01 00:00:00
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pub_type: 杂志文章
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更新日期:2007-05-01 00:00:00
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journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
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更新日期:1999-04-01 00:00:00
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journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/BF00229431
更新日期:1986-05-01 00:00:00
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journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
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更新日期:2008-01-01 00:00:00
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journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
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更新日期:2014-12-01 00:00:00
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journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:
更新日期:2003-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:2002-12-01 00:00:00
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journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
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更新日期:2001-05-01 00:00:00
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pub_type: 杂志文章
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更新日期:2019-02-01 00:00:00