当前位置： SCI文献检索 > MOLECULAR AND CELLULAR BIOCHEMISTRY期刊下所有文献 > False responses of Renilla luciferase reporter control to nuclear receptor TR4.

False responses of Renilla luciferase reporter control to nuclear receptor TR4.

Abstract：

:Renilla luciferase reporter is a widely used internal control in dual luciferase reporter assay system, where its transcription is driven by a constitutively active promoter. However, the authenticity of the Renilla luciferase response in some experimental settings has recently been questioned. Testicular receptor 4 (TR4, also known as NR2C2) belongs to the subfamily 2 of nuclear receptors. TR4 binds to a direct repeat regulatory element in the promoter of a variety of target genes and plays a key role in tumorigenesis, lipoprotein regulation, and central nervous system development. In our experimental system using murine pituitary corticotroph tumor AtT20 cells to investigate TR4 actions on POMC transcription, we found that overexpression of TR4 resulted in reduced Renilla luciferase expression whereas knockdown TR4 increased Renilla luciferase expression. The TR4 inhibitory effect was mediated by the TR4 DNA-binding domain and behaved similarly to the GR and its agonist, Dexamethasone. We further demonstrated that the chimeric intron, commonly present in various Renilla plasmid backbones such as pRL-Null, pRL-SV40, and pRL-TK, was responsible for TR4's inhibitory effect. The results suggest that an intron-free Renilla luciferase reporter may provide a satisfactory internal control for TR4 at certain dose range. Our findings advocate caution on the use of Renilla luciferase as an internal control in TR4-directed studies to avoid misleading data interpretation.

journal_name

Mol Cell Biochem

journal_title

Molecular and cellular biochemistry

authors

Zhang D,Atlasi SS,Patel KK,Zhuang Z,Heaney AP

doi

10.1007/s11010-017-2961-9

subject

Has Abstract

pub_date

2017-06-01 00:00:00

pages

139-147

issue

1-2

eissn

0300-8177

issn

1573-4919

pii

10.1007/s11010-017-2961-9

journal_volume

430

pub_type

杂志文章

相关文献

MOLECULAR AND CELLULAR BIOCHEMISTRY文献大全
  • Mechanisms of transcriptional activation of cAMP-responsive element-binding protein CREB.

    abstract：:The CREB-CREM transcription factors are the main gene regulatory effectors of the cAMP signaling pathway. The investigations of this family of transcription factors had a profound impact on the understanding of signaling-induced gene transcription. Here we discuss some key aspects of the underlying biology, review tra...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章,评审

    doi：

    authors： Haus-Seuffert P,Meisterernst M

    更新日期：2000-09-01 00:00:00

  • Characterization of muscarinic acetylcholine receptors on intact neuroblastoma x glioma NG108-15 cell upon induced differentiation.

    abstract：:Studies have established that major increases in muscarinic acetylcholine receptor (mAchR) binding in the brain appear to coincide with synaptogenesis. The neuroblastoma x glioma hybrid NG108-15 cell line has been demonstrated to possess numerous functional characteristics of intact neurons, including synapse formatio...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/BF00222618

    authors： Ghahary A,Cheng KW

    更新日期：1989-04-11 00:00:00

  • Genomic organization and neonatal expression of the bovine myostatin gene.

    abstract：:Myostatin belongs to the Transforming Growth Factor-beta (TGF-beta) superfamily and is expressed in developing and mature skeletal muscle. Biologically, the role of myostatin seems to be extremely well conserved during evolution since inactivating mutations in myostatin gene cause similar phenotype of heavy muscling i...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1023/a:1010801511963

    authors： Jeanplong F,Sharma M,Somers WG,Bass JJ,Kambadur R

    更新日期：2001-04-01 00:00:00

  • Silibinin-induced autophagy mediated by PPARα-sirt1-AMPK pathway participated in the regulation of type I collagen-enhanced migration in murine 3T3-L1 preadipocytes.

    abstract：:Preadipocyte migration is a fundamental and important process for the development of tissue organization, especially in the development of primitive adipose tissue and adipocyte tissue wound healing. However, excessive migration may result in abnormal development and fibrosis-related diseases such as hypertrophic scar...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-018-3368-y

    authors： Liu X,Xu Q,Long X,Liu W,Zhao Y,Hayashi T,Hattori S,Fujisaki H,Ogura T,Tashiro SI,Onodera S,Yamato M,Ikejima T

    更新日期：2019-01-01 00:00:00

  • Membrane bound and soluble adenosine triphosphatase of Escherichia coli K 12. Kinetic properties of the basal and trypsin-stimulated activities.

    abstract：:Basal and trypsin-stimulated adenosine triphosphatase activities of Escherichia coli K 12 have been characterized at pH 7.5 in the membrane-bound state and in a soluble form of the enzyme. The saturation curve for Mg2+/ATP = 1/2 was hyperbolic with the membrane-bound enzyme and sigmoidal with the soluble enzyme. Tryps...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/BF01732200

    authors： Carreira J,Muñoz E

    更新日期：1975-11-14 00:00:00

  • Characterization of apoA-I-dependent lipid efflux from adipocytes and role of ABCA1.

    abstract：:Adipose tissue is a major reservoir of cholesterol and, as such, it may play a significant role in cholesterol homeostasis. The aims of this study were to obtain a quantitative characterization of apolipoprotein A-I (apoA-I)-dependent lipid efflux from adipocytes and examine the role of ATP-binding cassette transporte...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-010-0505-7

    authors： Howard AD,Verghese PB,Arrese EL,Soulages JL

    更新日期：2010-10-01 00:00:00

  • Variables of myocardial backdiffusion, determined with 17-iodo-131 heptadecanoic acid in the normal dog heart.

    abstract：:Under normal and ischemic conditions backdiffusion of radiolabeled non-esterified fatty acids (NEFA) has been demonstrated. In the fasted normal canine heart the extraction fraction (EF) during interventions with glucose or lactate loading, vasodilation, and metabolic level augmentation was determined, and compared wi...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/BF00223443

    authors： Duwel CM,Visser FC,van Eenige MJ,Roos JP

    更新日期：1989-06-27 00:00:00

  • Metabolic phenotyping of the diseased rat heart using 13C-substrates and ex vivo perfusion in the working mode.

    abstract：:The objective of the present study was to compare energy substrate fluxes through metabolic pathways leading to mitochondrial citrate synthesis and release in normal and diseased rat hearts using 13C-substrates and mass isotopomer analysis by gas chromatography-mass spectrometry (GCMS). This study was prompted by our ...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：

    authors： Vincent G,Khairallah M,Bouchard B,Des Rosiers C

    更新日期：2003-01-01 00:00:00

  • Increased superoxide anion production is associated with early atherosclerosis and cardiovascular dysfunctions in a rabbit model.

    abstract：OBJECTIVE:Hypercholesterolemia (HC) has been associated with impairment of vascular and myocardial functions. As HC could generate an alteration in the oxidative status, we studied the effects of a 1-month cholesterol diet on cardiovascular oxidative stress. METHODS AND RESULTS:New Zealand rabbits received cholesterol...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-006-9263-y

    authors： Collin B,Busseuil D,Zeller M,Perrin C,Barthez O,Duvillard L,Vergely C,Bardou M,Dumas M,Cottin Y,Rochette L

    更新日期：2007-01-01 00:00:00

  • New perspectives in triple-negative breast cancer therapy based on treatments with TGFβ1 siRNA and doxorubicin.

    abstract：:Triple-negative breast cancer (TNBC), which accounts for 10-20% of all breast cancers, has the worst prognosis. Although chemotherapy treatment is a standard for TNBC, it lacks a specific target. Therefore, new therapeutic strategies are required to be investigated. In this study, a combined doxorubicin (DOX) and smal...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-020-03881-w

    authors： Ciocan-Cȃrtiţă CA,Jurj A,Raduly L,Cojocneanu R,Moldovan A,Pileczki V,Pop LA,Budişan L,Braicu C,Korban SS,Berindan-Neagoe I

    更新日期：2020-12-01 00:00:00

  • Liver-specific ZP domain-containing protein (LZP) as a new partner of Tamm-Horsfall protein harbors on renal tubules.

    abstract：:Liver-specific ZP domain-containing protein (LZP) was recently identified as a secreted protein that is specifically expressed in liver. However, the physiological effects of LZP are largely unknown. In this study, we found that LZP was detectable in mouse kidneys, testes, ovaries and heart, in addition to liver. LZP ...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-008-9921-3

    authors： Shen HL,Xu ZG,Huang LY,Liu D,Lin DH,Cao JB,Zhang X,Wang ZQ,Wang WH,Yang PY,Han ZG

    更新日期：2009-01-01 00:00:00

  • Glioma: what is the role of c-Myc, hsp90 and telomerase?

    abstract：:The continuous advancements in cancer research have contributed to the overwhelming evidence of the presence of telomerase in primary and secondary tumours together with hsp90 and c-Myc. This review will discuss the important role of telomerase together with hsp90 and c-Myc within the initiation and progression of gli...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章,评审

    doi：10.1007/s11010-006-2495-z

    authors： Shervington A,Cruickshanks N,Wright H,Atkinson-Dell R,Lea R,Roberts G,Shervington L

    更新日期：2006-02-01 00:00:00

  • Cysteine-rich secretory protein 3 plays a role in prostate cancer cell invasion and affects expression of PSA and ANXA1.

    abstract：:Cysteine-rich secretory protein 3 (CRISP-3) is upregulated in prostate cancer as compared to the normal prostate tissue. Higher expression of CRISP-3 has been linked to poor prognosis and hence it has been thought to act as a prognostic marker for prostate cancer. It is proposed to have a role in innate immunity but i...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-015-2564-2

    authors： Pathak BR,Breed AA,Apte S,Acharya K,Mahale SD

    更新日期：2016-01-01 00:00:00

  • The expression of monocyte chemotactic protein (MCP-1) in human vascular endothelium in vitro and in vivo.

    abstract：:A monocyte chemotactic protein (MCP-1) is thought to play a major role in recruiting monocytes to the vascular endothelium where the adherence of monocytes is one of the earliest events in atherogenesis. We cloned MCP-1 cDNA from a lambda gt 11 cDNA library constructed from human aortic endothelial mRNA to test whethe...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/BF01772208

    authors： Li YS,Shyy YJ,Wright JG,Valente AJ,Cornhill JF,Kolattukudy PE

    更新日期：1993-09-08 00:00:00

  • In vivo effect of vitamin E on serum and tissue glycoprotein levels in perchloroethylene induced cytotoxicity.

    abstract：:The antioxidant efficacy of vitamin E on Perchloroethylene (PER) induced cytotoxicity has been studied in rats. Feeding PER to rats for 42 days using sesame oil as vehicle alters total protein and protein bound carbohydrate components in liver and kidney of experimental animals. Supplementation of vitamin E prevented ...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/BF00926735

    authors： Ebrahim AS,Gopalakrishnan R,Murugesan A,Sakthisekaran D

    更新日期：1995-03-09 00:00:00

  • Effect of a dianthin G analogue in the differentiation of rat bone marrow mesenchymal stem cells into cardiomyocytes.

    abstract：:Loss of cardiomyocytes due to myocardial infarction results in ventricular remodeling which includes non-contractile scar formation, which can lead to heart failure. Stem cell therapy aims to replace the scar tissue with the functional myocardium. Mesenchymal stem cells (MSCs) are undifferentiated cells capable of sel...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-020-03855-y

    authors： Qazi RE,Naeem N,Khan I,Qadeer Q,Shaheen F,Salim A

    更新日期：2020-12-01 00:00:00

  • Vitamin A supplementation inhibits chemiluminescence and lipid peroxidation in isolated rat liver microsomes and mitochondria.

    abstract：:In the present study we investigated if administration of vitamin A could protect rat liver microsomes and mitochondria from in vitro peroxidation. Appreciable decrease of chemiluminescence and lipid peroxidation was measured in microsomal membranes from rats receiving vitamin A, with respect to control animals. In me...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/BF00248464

    authors： Palacios A,Piergiacomi VA,Catalá A

    更新日期：1996-01-12 00:00:00

  • Critical segment of apocytochrome c for its insertion into membrane.

    abstract：:Apocytochrome c has a potent ability to insert spontaneously into membrane. To identify which sequences were critical for this insertion activity, a series of peptides N19, C8, C15 and C21, corresponding to sequences 1-19, 81-88, 74-88 and 68-88 of apocytochrome c, respectively, were synthesized and purified. Insertio...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1023/b:mcbi.0000038216.13105.08

    authors： Wang X,Han X,Yang F

    更新日期：2004-07-01 00:00:00

  • OCT4 mutations in human erythroleukemic cells: implications for multiple drug resistance (MDR) phenotype.

    abstract：:The OCT4 transcription factor is a crucial stem cells marker and it has been related to the cancer stem cells concept. Moreover, it has also been associated to the multiple drug resistance (MDR) phenotype. Our first results pointed out a straight relation between OCT4 and ABC transporters in K562-derivative MDR (Lucen...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-014-2260-7

    authors： Oliveira BR,Figueiredo MA,Trindade GS,Marins LF

    更新日期：2015-02-01 00:00:00

  • Signalling by protein kinase C isoforms in the heart.

    abstract：:Understanding transmembrane signalling process is one of the major challenge of the decade. In most tissues, since Fisher and Krebs's discovery in the 1950's, protein phosphorylation has been widely recognized as a key event of this cellular function. Indeed, binding of hormones or neurotransmitters to specific membra...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章,评审

    doi：10.1007/BF00227882

    authors： Pucéat M,Vassort G

    更新日期：1996-04-12 00:00:00

  • The content of lipoperoxidation products in normal and atherosclerotic human aorta.

    abstract：:To evaluate the role of lipid oxidation in atherogenesis the levels of lipid- and protein-bound products of peroxidation in normal and atherosclerotic areas of human aorta were investigated. The level of fluorescent (360/430 nm) lipid products was measured in chloroform-methanol extracts of aortic tissue. Normal intim...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1023/a:1012267520932

    authors： Tertov VV,Kaplun VV,Mikhailova IA,Suprun IV,Orekhov AN

    更新日期：2001-09-01 00:00:00

  • Redox signaling.

    abstract：:Reactive oxygen species (ROS) and reactive nitrogen species (RNS) have recently been shown to be involved in a multiplicity of physiological responses through modulation of signaling pathways. Some of the specific signaling components altered by reactive oxygen and nitrogen species (RONS) have begun to be identified. ...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章,评审

    doi：

    authors： Forman HJ,Torres M,Fukuto J

    更新日期：2002-05-01 00:00:00

  • Chemical and immunological properties of B. anthracis arginase and its metabolic involvement.

    abstract：:An arginase isolated from a capsulated Bacillus anthracis strain was highly purified and crystallized. The chemical and immunological characteristics of this enzyme re described. Some very important properties differ from those of another bacterial arginase, i.e. Staphylococcus aureus arginase, described in a previous...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/BF00285642

    authors： Soru E

    更新日期：1983-01-01 00:00:00

  • Insulin sensitivity in experimental cirrhosis.

    abstract：:To investigate insulin action in muscle and adipose tissue in hepatic cirrhosis, a recently described animal model was used. Dimethylnitrosamine administration induced histologically proven cirrhosis. Contrary to expectation, muscle strips from cirrhotic rats displayed increased insulin sensitivity both with respect t...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/BF00228281

    authors： Chowdhury SA,Taylor R

    更新日期：1989-08-15 00:00:00

  • Purification and characterization of liver cytochrome P-446 isolated from protein energy malnourished rats.

    abstract：:A liver cytochrome P-450 isozyme has been purified to homogeneity from protein-energy malnourished rats induced with beta-naphthoflavone (beta-NF). The purification steps included chromatography on DEAE-Sephadex-A-25, DEAE-cellulose (DE-53), hydroxylapatite (HA) and carboxymethyl-sephadex (CM) columns. The reduced car...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/BF00229392

    authors： Gil L,Vasquez H,Orellana M,Selkirk J,Wold F,Strobel H

    更新日期：1988-01-01 00:00:00

  • Reversal of the growth inhibitory effect of alpha-difluoromethylornithine by putrescine but not by other divalent cations.

    abstract：:Treatment with alpha-difluoromethylornithine (DFMO), an enzyme-activated irreversible inhibitor of ornithine decarboxylase (ODC), depletes the putrescine and spermidine content, and reduces the growth rate of Ehrlich ascites tumor cells. The addition of putrescine, which is the immediate precursor of spermidine, promp...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/BF00224773

    authors： Oredsson SM,Anehus S,Heby O

    更新日期：1984-09-01 00:00:00

  • Sequence analysis of a few species of termites (Order: Isoptera) on the basis of partial characterization of COII gene.

    abstract：:The present study was aimed to get the nucleotide sequences of a part of COII mitochondrial gene amplified from individuals of five species of Termites (Isoptera: Termitidae: Macrotermitinae). Four of them belonged to the genus Odontotermes (O. obesus, O. horni, O. bhagwatii and Odontotermes sp.) and one to Microterme...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-009-0152-z

    authors： Sobti RC,Kumari M,Sharma VL,Sodhi M,Mukesh M,Shouche Y

    更新日期：2009-11-01 00:00:00

  • HSF1 regulates expression of G-CSF through the binding element for NF-IL6/CCAAT enhancer binding protein beta.

    abstract：:Heat shock factor 1 (HSF1) is the major heat shock transcription factor and plays an essential role in mediating the cellular response to physiological and environmental stress. We found that LPS-induced expression of the granulocyte-colony stimulating factor (G-CSF) gene was upregulated in HSF1 knock-out (HSF1(-/-)) ...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-010-0624-1

    authors： Zhang L,Yang M,Wang Q,Liu M,Liang Q,Zhang H,Xiao X

    更新日期：2011-06-01 00:00:00

  • BNIP3 induces IL6 and calcineurin/NFAT3 hypertrophic-related pathways in H9c2 cardiomyoblast cells.

    abstract：:Ischemia/reperfusion injury causes cardiomyocyte apoptosis, ventricular remodeling, leading to a dilated heart. Hypoxia is one of the causes involved in ischemia damage, and BNIP3 is a hypoxia-inducible marker and also a sensor to induce mitochondria-dependent apoptosis. Recent reports discussed ablating BNIP3 can res...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-010-0578-3

    authors： Weng YJ,Kuo WW,Kuo CH,Tung KC,Tsai CH,Lin JA,Tsai FJ,Hsieh DJ,Huang CY,Hwang JM

    更新日期：2010-12-01 00:00:00

  • Antioxidant properties of myocardial fuels.

    abstract：:Oxidative metabolism of blood-borne fuels provides myocardium the energy required to sustain its contractile performance. Recent research has revealed that, in addition to supplying energy, certain fuels are able to detoxify harmful oxidants and bolster the myocardium's endogenous antioxidant defenses. These antioxida...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章,评审

    doi：10.1023/a:1026009519783

    authors： Mallet RT,Sun J

    更新日期：2003-11-01 00:00:00