Abstract：
:Cellular FLICE-like inhibitory protein (Flip) is a negative regulator of nuclear factor κB signaling which has been shown previously to complicate with cardiac hypertrophy. In the present study, we tested the hypothesis that the knockout of Flip would increase cardiac hypertrophy in vivo and in vitro. The effects of Flip knockout on cardiac hypertrophy were investigated using in vitro and in vivo models. Flip was downregulated in transverse aortic constriction (TAC)-induced animal hearts and cardiomyocytes that had been treated with angiotensin II or phenylephrine for 1 h. An in vivo, heart hypertrophy model, was performed by TAC in Flip knockdown and sham mice. The extent of hypertrophy of heart was quantitated by echocardiography, and further confirmed by pathological and molecular examination of heart tissue samples. Conditional knockout of Flip in the murine heart increases the hypertrophic response induced by TAC, whereas cardiac function was preserved with reduced Flip levels in response to hypertrophic stimuli. Western blot experiments further showed Flip knockout activated markedly ASK1/P38 signaling cascades in vivo and in vitro. In conclusion, Flip preserves cardiac functions and inhibits cardiac hypertrophy partially by blocking ASK1/P38 signaling.
journal_name
Mol Cell Biochemjournal_title
Molecular and cellular biochemistryauthors
Huang Y,Wu L,Wu J,Li Y,Hou Ldoi
10.1007/s11010-014-2175-3subject
Has Abstractpub_date
2014-12-01 00:00:00pages
87-95issue
1-2eissn
0300-8177issn
1573-4919journal_volume
397pub_type
杂志文章abstract：:The possibility of amino acids biosynthesis from sucrose, metabolites of Krebs cycle or glyoxylate and ammonium by intact bacteroids has been studied. The suspension of intact Rhizobium lupini bacteroids in phosphate buffer solution pH 7.8 was shown to catalyse the biosynthesis from sucrose and ammonium of some amino ...
journal_title：Molecular and cellular biochemistry
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doi：10.1007/BF00215586
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doi：
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journal_title：Molecular and cellular biochemistry
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：
更新日期：1998-03-01 00:00:00
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/BF00227210
更新日期：1983-01-01 00:00:00
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
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更新日期：2001-04-01 00:00:00
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/BF00926579
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章,评审
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
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更新日期：2019-02-01 00:00:00
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journal_title：Molecular and cellular biochemistry
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更新日期：2010-12-01 00:00:00
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
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更新日期：2007-10-01 00:00:00
abstract：:Cryptotanshinone (CPT) is a natural compound extracted from herbal medicine that has been previously shown to possess antitumor properties in various types of human cancer cells. In the present study, we examined the potential role of CPT in the treatment of colorectal cancer. Using SW480, HCT116, and LOVO colorectal ...
journal_title：Molecular and cellular biochemistry
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更新日期：2015-08-01 00:00:00
