Abstract:
:Triplex forming oligonucleotides (TFO) are known to be potential agents for modifying gene function. In most instances they are utilized for repression of transcription. However hybrid molecules containing cis-acting elements in a duplex DNA in a hairpin form contiguously with the TFO can bind transcription factors in vitro. In the present manuscript we demonstrate that hairpin-TFO can be employed in vivo for targeted activation of gene expression of two genes mapping on chromosome XI of Saccharomyces cerevisiae. The cis-acting GAL4 protein-binding site contained in the hairpin-TFO is targeted in vivo to the 5' upstream sequence of STE6 and CBT1 genes that are transcribed in opposite directions and share a poly(pu/py) sequence that can form triple helical structure. The hairpin-TFO is targeted to this site and promotes the activation of both the genes. These results demonstrate four important aspects relating to activation of gene expression: (i) accessibility of duplex DNA packaged into chromatin to triplex forming sequences in vivo, (ii) the potential use of hairpin-TFO in therapeutics by activation of transcription in vivo, (iii) Sharing of transcription factors between two genes transcribed in opposite directions and (iv) specific activation of genes even when their cognate site is not covalently linked to the gene being activated.
journal_name
Mol Cell Biochemjournal_title
Molecular and cellular biochemistryauthors
Ghosh MK,Katyal A,Chandra R,Brahmachari Vdoi
10.1007/s11010-005-7283-7keywords:
subject
Has Abstractpub_date
2005-10-01 00:00:00pages
147-55issue
1-2eissn
0300-8177issn
1573-4919journal_volume
278pub_type
杂志文章abstract::We measured the relative sliding velocity of cardiomyopathic hamster cardiac myosin on actin cables by using an in vitro motility assay system. We also investigated the relationship between the velocity and both myosin isozyme content and ATPase activity. Cardiac myosin was obtained from cardiomyopathic hamsters (BIO ...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/BF00926857
更新日期:1993-02-17 00:00:00
abstract::The influence of altered levels of endogenous catecholamines following adrenalectomy or 6-hydroxydopamine (6-OH) treatment (alone or in combination) on enzymatic (glutathione reductase, catalase, glutathione peroxidase and Cu, Zn superoxide dismutase) and non-enzymatic (glutathione) antioxidant components of heart, li...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:
更新日期:1995-11-08 00:00:00
abstract:BACKGROUND:We reported that urinary L-FABP reflected the progression of chronic kidney disease (CKD). This study is aimed to evaluate the clinical significance of urinary liver type fatty acid binding protein (L-FABP) as a biomarker for monitoring CKD. METHODS:Urinary L-FABP was measured using human L-FABP ELISA kit (...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/s11010-005-9047-9
更新日期:2006-03-01 00:00:00
abstract::β-Adrenergic receptor (β-AR) stimulation induces cardiac myocyte apoptosis and plays an important role in myocardial remodeling. Here we investigated expression of various apoptosis-related genes affected by β-AR stimulation, and examined first time the role of ataxia telangiectasia mutated kinase (ATM) in cardiac myo...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/s11010-011-0769-6
更新日期:2011-07-01 00:00:00
abstract::Deficient wound healing is a common multifactorial complication in diabetic patients, but the cellular and molecular mechanisms involved are poorly defined. In the present study, we analyzed the effects of hyperglycemia on integrins expression in rat dermal fibroblasts and addressed its role in cell adhesion and migra...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/s11010-016-2780-4
更新日期:2016-10-01 00:00:00
abstract::Population data have consistently demonstrated a correlation between circulating branched-chain amino acids (BCAA) and insulin resistance. Most recently valine catabolite, 3-hydroxyisobutyrate, has emerged as a potential cause of BCAA-mediated insulin resistance; however, it is unclear if valine independently promotes...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/s11010-020-03720-y
更新日期:2020-05-01 00:00:00
abstract::Since the glucose-lowering effects of vanadium could be related to increased muscle glycogen synthesis, we examined the in vivo effects of vanadium and insulin treatment on glycogen synthase (GS) activation in Zucker fatty rats. The GS fractional activity (GSFA), protein phosphatase-1 (PP1), and glycogen synthase kina...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1023/a:1016116700632
更新日期:2002-07-01 00:00:00
abstract::The normal translocation of nascent polypeptides into the lumen of the endoplasmic reticulum (ER) is thought to be aided in part by a translocon-associated protein (TRAP) complex consisting of 4 protein subunits. The association of mature proteins with the ER and Golgi, or other intracellular locales, such as lysosome...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1023/a:1026598516681
更新日期:2000-12-01 00:00:00
abstract::The choroid plexus (CP) participates in the synthesis, secretion and regulation of the cerebrospinal fluid, in the removal of its toxic compounds and in the regulation of the availability of essential metal ions to the brain. It expresses and secretes metallothioneins 1/2 (MT-1/2) which are key components in the maint...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/s11010-012-1547-9
更新日期:2013-04-01 00:00:00
abstract::Studies presented here show that cellular NAD, which we hypothesize to be the relevant biomarker of niacin status, is significantly lower in humans than in the commonly studied animal models of carcinogenesis. We show that nicotinamide and the resulting cellular NAD concentration modulate expression of the tumor suppr...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:
更新日期:1999-03-01 00:00:00
abstract::The interaction of deferiprone (1,2-dimethyl-3-hydroxy-pyrid-4-one) L1, the first clinically available oral iron chelator, with the tetrameric allosteric protein hemoglobin from human red blood cells has been investigated spectrofluorometrically and by circular dichroism spectroscopy. The interaction is hydrogenbond l...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1023/a:1007049701572
更新日期:2000-01-01 00:00:00
abstract::DEAE-cellulose column chromatography of Neurospora crassa soluble mycelial extracts leads to the resolution of three major protein kinase activity peaks designated PKI, PKII, and PKIII. PKII activity is stimulated by Ca2+ and Neurospora or brain calmodulin. Maximal stimulation was observed at 2 microM-free Ca2+ and 1 ...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/BF00234573
更新日期:1991-04-10 00:00:00
abstract::The present study was addressed to understand the interrelationship between Receptor-Ck activation, mevalonate pathway and primary response genes such as c-fos, c-myc and cyclin 'D' involved in the cell cycle. The results reported here unambiguously revealed that the phosphatidic acid (generated through the activation...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1023/a:1006890215502
更新日期:1998-04-01 00:00:00
abstract::The action of micrococcal nuclease, DNase I and DNase II on mouse TLT hepatoma chromatin revealing the periodicity of its structure as visualized by denaturing and non-denaturing gel electrophoresis, was consistent with the action of these enzymes on other chromatins. Micrococcal nuclease showed a complex subnucleosom...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/BF00232599
更新日期:1978-04-11 00:00:00
abstract::Although studies performed in vitro and with transfected cells in culture suggest a role for liver fatty acid binding protein (L-FABP) in regulating fatty acid oxidation and fat deposition, the physiological significance of this possibility is not completely clear. To begin to address this question, the effect of L-FA...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/s11010-008-9989-9
更新日期:2009-04-01 00:00:00
abstract::The marine blood clam species Anadara granosa (L) belong to arcidae, a family with some extraordinary haematological features. The plasma of this species exhibited strong haemagglutinating activities, from which a galactosyl binding lectin, Anadarin P, was purified in a single step affinity chromatography using Sephar...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/BF00230405
更新日期:1992-11-04 00:00:00
abstract::We have tested the hypothesis that activation of the insulin receptor tyrosine kinase is due to autophosphorylation of tyrosines 1146, 1150 and 1151 within a putative autoinhibitory domain. A synthetic peptide corresponding to residues 1134-1162, with tyrosines substituted by alanine or phenylalanine, of the insulin r...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/BF00926082
更新日期:1993-03-24 00:00:00
abstract::To define metabolic influences on cardiac myosin expression and sarcoplasmic reticulum (SR) Ca(2+)-stimulated ATPase streptozotocin-diabetic rats were treated for 9-10 wk with etomoxir, an inhibitor of carnitine palmitoyl transferase I (CPT-1) and fatty acid synthesis, or an antilipolytic drug, acipimox. Etomoxir redu...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/BF00925676
更新日期:1994-03-16 00:00:00
abstract::Increasing evidence has shown that normal stem cells may act as cancer-initiating cells and contribute to the development and progression of cancer. HBx has a close relationship with hepatocellular carcinoma, however, the role of HBx in hepatic progenitor cells (HPCs) is poorly understood. In this study, we sought to ...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/s11010-013-1769-5
更新日期:2013-11-01 00:00:00
abstract::This review examines the mechanism of translocation of cytoplasmically synthesized proteins into mitochondria. Approximately 10% of the mitochondrial proteins are synthesized within the organelles while most mitochondrial proteins are coded for by nuclear genes and synthesized on cytoplasmic ribosomes. Those mitochond...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/BF00423100
更新日期:1982-03-19 00:00:00
abstract::An increasing amount of experimental and epidemiological evidence implicates the involvement of oxygen derived radicals in the pathogenesis of cancer development. It is well known that chemical carcinogenesis is multistage process. Free radicals arefound to be involved in both initiation and promotion of multistage ca...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/s11010-005-0325-3
更新日期:2005-05-01 00:00:00
abstract::A quarter of the human population with European background carries at least one allele of the OPRD1 gene that encodes the delta opioid receptor with cysteine at the amino acid position 27 (hδOR(Cys27)) instead of the evolutionary conserved phenylalanine (hδOR(Phe27)). The two variants have indistinguishable pharmacolo...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/s11010-011-0725-5
更新日期:2011-05-01 00:00:00
abstract::The term leishmaniasis refers collectively to various clinical syndromes that are caused by obligate intracellular protozoa of the genus Leishmania. Approximately 350 million people in 8 countries are estimated to be threatened by the disease. The World Health Organization estimated that there are 12 million cases of ...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章,评审
doi:10.1023/a:1026047429604
更新日期:2003-11-01 00:00:00
abstract::Hepcidin is a cysteine-rich, dual-function peptide with antimicrobial activity that plays a crucial role in iron homeostasis. Here, we have identified two hepcidin-like cDNA sequences from pigeon, Columba livia. The two cDNAs consist of 295 and 380 nucleotides, respectively, and were named HP1 and HP2. Sequence alignm...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/s11010-007-9543-1
更新日期:2007-11-01 00:00:00
abstract::Utrophin is a widely expressed cytoskeleton protein and is associated with lipid droplets (LDs) in adipocytes. The scaffold protein beta 2 syntrophin (SNTB2) controls signaling events by recruiting distinct membrane and cytoskeletal proteins, and binds to utrophin. Here we show that SNTB2 forms a complex with utrophin...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/s11010-018-3409-6
更新日期:2019-02-01 00:00:00
abstract::Gastrointestinal symptoms are fairly common in diabetes mellitus. Glimepride, is a latest second generation sulfonylurea used for the treatment of type II diabetes mellitus, is a insulin secrectagogue; indirectly, it also increases insulin secretion and its specific effect on pancreatic ATP-dependent K(+) channel inhi...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/s11010-011-0812-7
更新日期:2011-08-01 00:00:00
abstract::Two alternatively spliced forms of human PPAR alpha mRNA, PPAR alpha1 and PPAR alpha2, have been identified. PPAR alpha1 mRNA gives rise to an active PPAR alpha protein while PPAR alpha2 mRNA gives rise to a form of PPAR which lacks the ligand-binding domain. PPAR alpha2 is unable to activate a peroxisome proliferator...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1023/a:1007248007372
更新日期:2001-01-01 00:00:00
abstract::MicroRNA-125b (miR-125b) reduces myocardial infarct area and restrains myocardial ischemia reperfusion injury (I/R). In this study, we aimed to investigate the effect of bone marrow mesenchymal stem cell (BMSC)-derived exosomes carrying miR-125b on I/R rats. The myocardial I/R model in rats was constructed by ligation...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/s11010-019-03671-z
更新日期:2020-02-01 00:00:00
abstract::Transforming growth factor-β1 (TGF-β1) has been thought to play a major role during cardiac fibrosis in the development of diabetic cardiomyopathy, and cardiac fibrosis mainly as a result of an increase of collagen type III occurs in the human hearts with diabetes. Thrombospondin-1 (TSP-1) has been reported to activat...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/s11010-010-0590-7
更新日期:2011-01-01 00:00:00
abstract::The treatment of obstructive sleep apnea-hypopnea syndrome targets the narrow anatomic structure of the upper airway (UA) and lacks an effective therapy for UA dilator muscle dysfunction. Long-term hypoxia can cause damage to UA dilator muscles and trigger a vicious cycle. We previously confirmed that hypoxia-inducibl...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/s11010-020-03995-1
更新日期:2021-01-03 00:00:00