Abstract：
:Studies presented here show that cellular NAD, which we hypothesize to be the relevant biomarker of niacin status, is significantly lower in humans than in the commonly studied animal models of carcinogenesis. We show that nicotinamide and the resulting cellular NAD concentration modulate expression of the tumor suppressor protein, p53, in human breast, skin, and lung cells. Studies to determine the optimal NAD concentrations for responding to DNA damage in breast epithelial cells reveal that DNA damage appears to stimulate NAD biosynthesis and that recovery from DNA damage occurs several hours earlier in the presence of higher NAD or in cells undergoing active NAD biosynthesis. Finally, analyses of normal human skin tissue from individuals diagnosed with actinic keratoses or squamous cell carcinomas show that NAD content of the skin is inversely correlated with the malignant phenotype. Since NAD is important in modulating ADP-ribose polymer metabolism, cyclic ADP-ribose synthesis, and stress response proteins, such as p53, following DNA damage, understanding how NAD metabolism is regulated in the human has important implications in developing both prevention and treatment strategies in carcinogenesis.
journal_name
Mol Cell Biochemjournal_title
Molecular and cellular biochemistryauthors
Jacobson EL,Shieh WM,Huang ACkeywords:
subject
Has Abstractpub_date
1999-03-01 00:00:00pages
69-74issue
1-2eissn
0300-8177issn
1573-4919journal_volume
193pub_type
杂志文章abstract：:In rat cardiac sarcolemmal membranes a phosphoinositide-specific phospholipase C (PLC) was found to be present. The enzyme hydrolysed exogenous [3H-]phosphatidylinositol 4,5-biphosphate ([3H-]PtdIns(4,5)P2) in an optimized assay mixture containing 15 micrograms SL protein, 100 mM NaCl, 1 mM free Ca2+, 14 mM Na-cholate...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
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abstract：:Recent studies have shown that adult osteoporosis could be induced by ageing and estrogen deficiency and homocysteine (Hcy) is an independent risk factor of fracture in osteoporosis patients. In this study, we found hypermethylation of the promoter A region in the estrogen receptor alpha (ERα) gene, and methylation in...
journal_title：Molecular and cellular biochemistry
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journal_title：Molecular and cellular biochemistry
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doi：10.1007/BF01084267
更新日期：1994-01-12 00:00:00
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/BF00224926
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journal_title：Molecular and cellular biochemistry
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章,评审
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章,评审
doi：
更新日期：2001-06-01 00:00:00
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
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journal_title：Molecular and cellular biochemistry
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journal_title：Molecular and cellular biochemistry
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更新日期：2009-03-01 00:00:00
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journal_title：Molecular and cellular biochemistry
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更新日期：1997-09-01 00:00:00
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更新日期：1997-09-01 00:00:00
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journal_title：Molecular and cellular biochemistry
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journal_title：Molecular and cellular biochemistry
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
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journal_title：Molecular and cellular biochemistry
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