Effects of N-(2-mercaptopropionyl)glycine on ischemic-reperfused dog kidney in vivo and membrane preparation in vitro.

abstract：:SIAH-1, an E3 ubiquitin ligase, plays an important role in regulating cell cycle, tumorigenesis and several neurodegenerative diseases. In this study, we found a novel SIAH-1-interacting protein, EEF1D (Eukaryotic translation elongation factor 1 delta). The interaction was confirmed in vitro and in vivo, and both prot...

journal_title：Molecular and cellular biochemistry

authors： Wu H,Shi Y,Lin Y,Qian W,Yu Y,Huo K

更新日期：2011-11-01 00:00:00

abstract：:The monoterpene perillyl alcohol (POH) is a drug used in the treatment of several malignant tumors, including gliomas. The present study defines a POH inhibitory effect on Na/K-ATPase activity from kidney and brain guinea pig extracts and from a human glioblastoma cell line. This inhibition showed a high degree of sel...

journal_title：Molecular and cellular biochemistry

authors： Garcia DG,Amorim LM,de Castro Faria MV,Freire AS,Santelli RE,Da Fonseca CO,Quirico-Santos T,Burth P

更新日期：2010-12-01 00:00:00

abstract：:We measured the relative sliding velocity of cardiomyopathic hamster cardiac myosin on actin cables by using an in vitro motility assay system. We also investigated the relationship between the velocity and both myosin isozyme content and ATPase activity. Cardiac myosin was obtained from cardiomyopathic hamsters (BIO ...

journal_title：Molecular and cellular biochemistry

authors： Yamashita H,Sugiura S,Sata M,Serizawa T,Iizuka M,Shimmen T,Momomura S

更新日期：1993-02-17 00:00:00

abstract：:In mammalian cells, increases in calcium concentration cause increases in oxidative phosphorylation. This effect is mediated by the activation of four mitochondrial dehydrogenases by calcium ions; FAD-glycerol 3-phosphate dehydrogenase, pyruvate dehydrogenase, NAD-isocitrate dehydrogenase and oxoglutarate dehydrogenas...

journal_title：Molecular and cellular biochemistry

authors： Nichols BJ,Denton RM

更新日期：1995-08-01 00:00:00

abstract：:The purified Ca2+/Mg2+ ATPase from rat heart plasma membrane was activated by Ca2+ and Mg2+ with Ka values of 1.47 mM and 2.51 mM, respectively; other divalent cations also activated the enzyme but to a lesser extent. Divalent cations like Cu2+, Zn2+, Ni2+, Cd2+ were potent inhibitors of the enzyme activity in the pre...

journal_title：Molecular and cellular biochemistry

authors： Zhao D,Elimban V,Dhalla NS

更新日期：1991-10-16 00:00:00

abstract：:Twenty-six days of fat deficiency brought about a decrease of linoleic and an increase of oleic acid in rough endoplasmic reticulum (RER) of guinea pig liver. Arachidonic acid was only slightly decreased in some phospholipids whereas eicose-5,8,11-trienoic acid was not enhanced except in phosphatidyl-inositol. All the...

journal_title：Molecular and cellular biochemistry

authors： Soulages JL,Brenner RR

更新日期：1987-12-01 00:00:00

abstract：:HMGb1 is a DNA-binding protein whose role as an extracellular cytokine in inflammation and tissue regeneration has also been reported. Given the importance of keratinocytes in wound healing, we have studied the mechanism of action of HMGb1 on HaCaT keratinocytes during in vitro scratch wound repair. Western blot and c...

journal_title：Molecular and cellular biochemistry

authors： Ranzato E,Patrone M,Pedrazzi M,Burlando B

更新日期：2009-12-01 00:00:00

abstract：:Tedisamil is antiarrhythmic class III drug with antifibrillating/defibrillating potency linked to enhancement of intermyocyte gap junctional electrical coupling most likely via its sympathomimetic cAMP-related mechanisms. This study was designed to examine the effect of tedisamil on cAMP level in guinea pig hearts in ...

journal_title：Molecular and cellular biochemistry

authors： Tribulova N,Ravingerova T,Okruhlicova L,Gabauer I,Fickova M,Pancza D,Slezak J,Manoach M

更新日期：2000-07-01 00:00:00

abstract：:Transforming growth factor-β1 (TGF-β1) has been thought to play a major role during cardiac fibrosis in the development of diabetic cardiomyopathy, and cardiac fibrosis mainly as a result of an increase of collagen type III occurs in the human hearts with diabetes. Thrombospondin-1 (TSP-1) has been reported to activat...

journal_title：Molecular and cellular biochemistry

authors： Tang M,Zhou F,Zhang W,Guo Z,Shang Y,Lu H,Lu R,Zhang Y,Chen Y,Zhong M

更新日期：2011-01-01 00:00:00

abstract：:ADP-ribosylation factors (ARFs) comprise a family of approximately 20 kDa guanine nucleotide-binding proteins that were discovered as one of several cofactors required in cholera toxin-catalyzed ADP-ribosylation of Gs alpha, the guanine nucleotide-binding protein responsible for stimulation of adenylyl cyclase, and wa...

journal_title：Molecular and cellular biochemistry

authors： Welsh CF,Moss J,Vaughan M

更新日期：1994-09-01 00:00:00

abstract：:Tripeptide aminopeptidase (EC 3.4.11.4) was purified from bovine dental follicles by a series of chromatographies. Purified enzyme had a specific activity of 59.5 units per mg protein with L-prolyl-glycylglycine as substrate. The pH optimum was 8.0. The purified native enzyme had a Mr of 230,000 and was shown to be a ...

journal_title：Molecular and cellular biochemistry

authors： Hiraoka BY,Harada M

更新日期：1993-12-08 00:00:00

abstract：:Persistent activation of nuclear factor B (NF-κB) is very important in the modulation of macrophages cellular response to microbial infections. The deubiquitinase USP14, which is critical for ubiquitin-mediated proteasomal degradation of proteins, is known to be involved in cancer, neurological diseases, and aging. Ho...

journal_title：Molecular and cellular biochemistry

authors： Liu N,Kong T,Chen X,Hu H,Gu H,Liu S,Chen X,Yang Q,Li A,Xiong X,Zhang Z

更新日期：2017-07-01 00:00:00

abstract：:The effects of Mg2+ concentration (Mg2+o, 0, 1.2, 2.4, and 4.8 mM) on the incidence of reperfusion arrhythmias and on the cellular electrical activity were studied in spontaneously beating rat hearts. The surface electrogram and the membrane potential were recorded in control conditions, during 10 min of regional isch...

journal_title：Molecular and cellular biochemistry

authors： Ponce Zumino A,Risler NR,Schanne OF,Ruiz Petrich E,Carrión A

更新日期：1997-06-01 00:00:00

abstract：:Cervical cancer is the most common cancer in Indian females and is associated with infection with high risk Human papillomaviruses (HPVs). Curcumin (Diferuloyl methane), a chemopreventive agent, is a natural compound extracted from Curcuma longa that allows suppression of carcinogenesis. The objective of this study wa...

journal_title：Molecular and cellular biochemistry

authors： Singh M,Singh N

更新日期：2009-05-01 00:00:00

abstract：:A crude preparation of heavy sarcoplasmic reticulum (HSR) was isolated using 1 gram of muscle obtained from swine susceptible to malignant hyperthermia (MH) and from control swine. The caffeine and ATP concentration-dependency of Ca-release was determined using suction filtration with radioisotopic 45Ca as a tracer. R...

journal_title：Molecular and cellular biochemistry

authors： O'Brien PJ

更新日期：1990-03-05 00:00:00

abstract：:Evidence suggests that functional atrial natriuretic peptide (ANP) receptors occur in surface gastric mucosal epithelial cells. To evaluate functional aspects of ANP in a model of these cells we examined the expression of natriuretic peptide receptors (NPR) subtypes A and C in the non-transformed rat gastric mucosal e...

journal_title：Molecular and cellular biochemistry

authors： Gower WR Jr,Carter GM,McAfee Q,Solivan SM

更新日期：2006-12-01 00:00:00

abstract：:Differentiation of 3T3-L1 preadipocytes, induced by methyl-isobutylxanthine (MIX), dexamethasone (DEX), and insulin, results in cells with the morphological and biochemical characteristics of adipocytes. Following incubation of 3T3-L1 cells with MIX, DEX, and insulin, poly(ADP-ribose) synthetase activity decreased abr...

journal_title：Molecular and cellular biochemistry

authors： Pekala PH,Moss J

更新日期：1983-01-01 00:00:00

abstract：:Logistic complexities of heart transplantation embossed the necessity of utilizing novel methods, which enable heart regeneration. Human cardiosphere-derived cells (hCDCs) are taken into consideration as a promising cell resource in cell therapy in recent years. In this study, we designed an electrochemical stimulatio...

journal_title：Molecular and cellular biochemistry

authors： Nazari H,Kehtari M,Rad I,Ashtari B,Joghataei MT

更新日期：2020-07-01 00:00:00

abstract：:Fat cells were preincubated for 2 h in the presence and absence of growth hormone (GH) and Dexamethasone (Dex) before the addition of increasing concentrations of either epinephrine, theophylline or glucagon and final incubation of the cells for an additional 5 minutes. GH and Dex increased by 85%, 28% and 72%, respec...

journal_title：Molecular and cellular biochemistry

authors： Harris R,Bennun A

更新日期：1976-12-10 00:00:00

abstract：:One of the most clinically relevant biological activities of curcumin is its anti-cancer property, implicating multiple intracellular pathways in the process. In the present report, we investigated the effect of curcumin on the activation of apoptotic and anti-angiogenic pathways in Ehrlich Ascites Tumor (EAT) cells. ...

journal_title：Molecular and cellular biochemistry

authors： Belakavadi M,Salimath BP

更新日期：2005-05-01 00:00:00

abstract：:In vivo administration of pyridine has been shown to increase the activity and content of several forms of cytochrome P450 by transcriptional and posttranscriptional mechanisms. The effect of pyridine on CYP1A and CYP2E1 isozymes was studied in a rat hepatocyte culture model. Hepatocytes were isolated from non-induced...

journal_title：Molecular and cellular biochemistry

authors： Wu D,Ramin SA,Cederbaum AI

更新日期：1997-08-01 00:00:00

abstract：:Arjunolic acid, a new triterpene and a potent principle from the bark of Terminalia arjuna, has been shown to provide significant cardiac protection in isoproterenol induced myocardial necrosis in rats. To further explore the mechanism of action of arjunolic acid, antiplatelet activity, anticoagulant assays, electroca...

journal_title：Molecular and cellular biochemistry

authors： Sumitra M,Manikandan P,Kumar DA,Arutselvan N,Balakrishna K,Manohar BM,Puvanakrishnan R

更新日期：2001-08-01 00:00:00

abstract：UNLABELLED:Carnosic acid is a well-known antioxidant. Recently, it has been identified as modulator of nuclear factor erythroid 2-related factor 2 (Nrf2). The effect of carnosic acid in the context of cardiovascular disorders has not been studied. In the present study, we investigated the beneficial effect and the unde...

journal_title：Molecular and cellular biochemistry

authors： Sahu BD,Putcha UK,Kuncha M,Rachamalla SS,Sistla R

更新日期：2014-09-01 00:00:00

abstract：:We firstly characterized zinc uptake phenomenon across basolateral membrane vesicles (BLMVs) isolated from normal rat kidney. The process was found to be time, temperature, and substrate concentration dependent, and displayed saturability. Zn(2+) uptake was competitively inhibited in the presence of 2 mM Cd with Ki of...

journal_title：Molecular and cellular biochemistry

authors： Kaur J,Sharma N,Attri S,Gogia L,Prasad R

更新日期：2006-02-01 00:00:00

abstract：:The amylase-protein amylase inhibitor system offers a unique model of specific and reversilbe protein-protein interaction. The monomeric and dimeric inhibitors, exhibiting closely related properties and interacting with the same amylase, also provide a convenient test to compare effects of monomer-monomer and monomer-...

journal_title：Molecular and cellular biochemistry

authors： Silano V,Poerio E,Buonocore V

更新日期：1977-12-29 00:00:00

abstract：:To examine the essential mechanisms of steroid production in ovarian theca cells, we analyzed the expression of genes associated with steroid production using simple culture system with serum medium. In addition, we examined the involvement of DAX-1, COUP-TFII, and Ad4BP/SF-1 transcription factors on the steroid produ...

journal_title：Molecular and cellular biochemistry

authors： Murayama C,Miyazaki H,Miyamoto A,Shimizu T

更新日期：2008-07-01 00:00:00

abstract：:Plexiform neurofibromas commonly found in patients with Neurofibromatosis type I (NF1) have a 5% risk of being transformed into malignant peripheral nerve sheath tumors (MPNST). Germline mutations in the NF1 gene coding for neurofibromin, which is a Ras GTPase activating protein (RasGAP) and a negative regulator of Ra...

journal_title：Molecular and cellular biochemistry

authors： Kraniak JM,Sun D,Mattingly RR,Reiners JJ Jr,Tainsky MA

更新日期：2010-11-01 00:00:00

abstract：:Under synchronized conditions daily rhythms run in precise phase relationships. Long lasting shift-work disturbs circadian rhythms and causes metabolism dysfunction. As a result of frequent shifts of the light (L):dark (D) cycle the circadian system has to adjust to a new regimen repeatedly, and organism can never ach...

journal_title：Molecular and cellular biochemistry

authors： Szántóová K,Zeman M,Veselá A,Herichová I

更新日期：2011-02-01 00:00:00

abstract：:Endothelin-1 (ET-1), a peptide isolated from the culture medium of endothelial cells, mediates a variety of physiological and pathological responses including mitogenesis. We have compared the expression of ET receptors in untransformed versus ras-transformed NIH-3T3 murine fibroblasts and in untransformed versus SV40...

journal_title：Molecular and cellular biochemistry

authors： Nambi P,Mattern MR,Wu HL,Pullen M,Nuthulaganti P,Hofmann GA,Kumar C

更新日期：1997-10-01 00:00:00

abstract：:MicroRNAs (miRNAs, miRs) play important roles in pathogenesis and development of human diseases, including malignancy. Some may affect tumor progression through targeting tumor suppressor genes. MiR-135b has been shown to be upregulated in CRC. In this study, we evaluated the role of miR-135b in colorectal cancer (CRC...

journal_title：Molecular and cellular biochemistry

authors： Wu W,Wang Z,Yang P,Yang J,Liang J,Chen Y,Wang H,Wei G,Ye S,Zhou Y

更新日期：2014-03-01 00:00:00