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Effects of fatty acid deficiency on the lipid composition and physical properties of guinea pig rough endoplasmic reticulum.

Abstract：

:Twenty-six days of fat deficiency brought about a decrease of linoleic and an increase of oleic acid in rough endoplasmic reticulum (RER) of guinea pig liver. Arachidonic acid was only slightly decreased in some phospholipids whereas eicose-5,8,11-trienoic acid was not enhanced except in phosphatidyl-inositol. All these changes were relevant specifically in phosphatidylinositol molecules and less important in phosphatidylcholine and phosphatidylethanolamine. Fat deficiency did not modify the relative proportion of phospholipids and cholesterol. Therefore, fat deficient guinea pig microsomes are a good model to study the effect of unsaturated fatty acids on membrane properties. Fluorescent anisotropy of RER membranes, lipids and phospholipids labeled with diphenylhexatriene, was increased by the fat deficiency. The most important increase was observed in liposomes of a mixture of RER phosphatidylinositol, phosphatidylserine and sphingomyelin. A small change was found in phosphatidylcholine and phosphatidylethanolamine dispersions at 37 degrees C. The modification of the lipid unsaturation evoked fluorescent anisotropy changes. Temperature-dependent fluorescent polarization curves of RER membranes labeled with trans-parinaric acid did not show inflections in the temperature range from 5 to 45 degrees C but, RER lipids and phospholipids presented a phase separation at about 20 degrees C. This inflection point was not modified by the fat deficient diet. In those liposomes prepared with a mixture of RER phosphatidylinositol, phosphatidylserine and sphingomyelin, the inflection point was produced at about 37 degrees C.

journal_name

Mol Cell Biochem

journal_title

Molecular and cellular biochemistry

authors

Soulages JL,Brenner RR

doi

10.1007/BF00229685

subject

Has Abstract

pub_date

1987-12-01 00:00:00

pages

109-19

issue

2

eissn

0300-8177

issn

1573-4919

journal_volume

78

pub_type

杂志文章

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