Abstract：
:Logistic complexities of heart transplantation embossed the necessity of utilizing novel methods, which enable heart regeneration. Human cardiosphere-derived cells (hCDCs) are taken into consideration as a promising cell resource in cell therapy in recent years. In this study, we designed an electrochemical stimulation system, which sends square pulses to the hCDCs and records their electrical response. Morphology, viability and differentiation of hCDCs are monitored at certain time courses of the treatment. Differentiating hCDCs aligned perpendicularly with respect to the direction of applied electric current, and obtained a spindle-like morphology, while they remained viable. At the same time, specific cardiac marker genes including GATA4, cTnT and α-MHC showed a considerable up-regulation. Our findings confirm that hCDCs differentiate to committed cardiomyocytes when hCDCs receive an electrical energy of 0.06 - 0.12 Wh. This amount of electrical energy could be applied to the stem cells using versatile electrical stimulation patterns via commercially available devices.
journal_name
Mol Cell Biochemjournal_title
Molecular and cellular biochemistryauthors
Nazari H,Kehtari M,Rad I,Ashtari B,Joghataei MTdoi
10.1007/s11010-020-03742-6subject
Has Abstractpub_date
2020-07-01 00:00:00pages
29-39issue
1-2eissn
0300-8177issn
1573-4919pii
10.1007/s11010-020-03742-6journal_volume
470pub_type
杂志文章abstract：:Endothelial progenitor cell (EPC) transplantation has shown advantages in the treatment of myocardial infarction (MI) in animal models and clinical trials through mechanisms of direct intercellular contacts, autocrine, and paracrine. However, the effects of EPC transplantation for MI treatment remain controversial and...
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/BF00224773
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journal_title：Molecular and cellular biochemistry
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journal_title：Molecular and cellular biochemistry
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章,评审
doi：10.1007/BF00285225
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/BF00223443
更新日期：1989-06-27 00:00:00
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journal_title：Molecular and cellular biochemistry
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doi：10.1023/a:1020648425895
更新日期：2002-11-01 00:00:00
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journal_title：Molecular and cellular biochemistry
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doi：10.1007/BF00285223
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更新日期：2004-08-01 00:00:00
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journal_title：Molecular and cellular biochemistry
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