HMGb1 promotes scratch wound closure of HaCaT keratinocytes via ERK1/2 activation.


:HMGb1 is a DNA-binding protein whose role as an extracellular cytokine in inflammation and tissue regeneration has also been reported. Given the importance of keratinocytes in wound healing, we have studied the mechanism of action of HMGb1 on HaCaT keratinocytes during in vitro scratch wound repair. Western blot and confocal immunofluorescence microscopy showed that these cells express significant amounts of HMGb1, that the protein is prevalently localized in the nucleus, and that its release by cells is negligible. Western blot also showed that these cells express the HMGb1 receptor RAGE. Cell exposure to HMGb1 in the absence of serum resulted in a stimulation of cell proliferation and ERK1/2 activation. HMGb1 also accelerated the wound closure of scratch wounded cells and promoted cell migration, as evaluated by a transwell assay. The HMGb1-induced increases of cell proliferation, cell migration, and wound closure were abolished by the MEK inhibitor PD98059. Taken together, data show that, although HMGb1 is not released by HaCaT, when applied exogenously it can induce a marked increase of the wound repair of these cells. Data also suggest that HMGb1 acts via the RAGE/MEK/ERK pathway. These results bring scientific support to the potential application of HMGb1 in regenerative medicine.


Mol Cell Biochem


Ranzato E,Patrone M,Pedrazzi M,Burlando B




Has Abstract


2009-12-01 00:00:00












  • Effect of pentoxifylline on arachidonic acid metabolism, neutral lipid synthesis and accumulation during induction of the lipocyte phenotype by retinol in murine hepatic stellate cell.

    abstract::In liver fibrosis, the quiescent hepatic stellate cells (HSC) are activated to proliferate and express the activated myofibroblast phenotype, losing fat droplets and the stored vitamin A, and depositing more extracellular matrix. Therapeutic strategies for liver fibrosis are focused on HSC. Pentoxifylline (PTF), an an...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Cardoso CC,Paviani ER,Cruz LA,Guma FC,Borojevic R,Guaragna RM

    更新日期:2003-12-01 00:00:00

  • The transcription factor TBX2 regulates melanogenesis in melanocytes by repressing Oca2.

    abstract::The T-box transcription factor TBX2 is known for its role as a critical regulator of melanoma cell proliferation, but its role in regulating melanogenesis has not been widely studied. Here we use a series of experiments to show in primary and immortalized mouse melanocytes that TBX2 acts as regulator of melanogenesis ...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Chen Y,Pan L,Su Z,Wang J,Li H,Ma X,Liu Y,Lu F,Qu J,Hou L

    更新日期:2016-04-01 00:00:00

  • Combined efficacy of tamoxifen and coenzyme Q10 on the status of lipid peroxidation and antioxidants in DMBA induced breast cancer.

    abstract::An increasing amount of experimental and epidemiological evidence implicates the involvement of oxygen derived radicals in the pathogenesis of cancer development. It is well known that chemical carcinogenesis is multistage process. Free radicals arefound to be involved in both initiation and promotion of multistage ca...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Perumal SS,Shanthi P,Sachdanandam P

    更新日期:2005-05-01 00:00:00

  • Effect of albumin on acyl-CoA: lysolecithin acyltransferase, lysolecithin: lysolecithin acyltransferase and acyl-CoA hydrolase from rabbit lung.

    abstract::Acyl-CoA: lysolecithin and lysolecithin: lysolecithin acyltransferases, as well as acyl-CoA hydrolase are important enzymes in lung lipid metabolism. They use amphiphylic lipids as substrates and differ in subcellular localization. In this sense, lipid-protein interactions can be an essential factor in their activity....

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Pérez-Gil J,Estrada P,Acebal C,Arche R

    更新日期:1990-05-10 00:00:00

  • Nucleoside phosphotransferase of chick embryo.

    abstract::This paper describes a purification procedure and some properties of a nonspecific nucleoside phosphotransferase of chick embryo, an activity which catalyzes the transfer of chick embryo, an activity which catalyzes the transfer of the phosphate ester from a deoxyribonucleotide or a pyrimidine ribonucleotide to a deox...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Tesoriere G,Vento R,Calvaruso G,Taibi G

    更新日期:1979-06-15 00:00:00

  • Regulation of low density lipoprotein receptor mRNA levels by estradiol 17beta and chorionic gonadotropin in human placenta.

    abstract::Inhibition of synthesis of estradiol 17beta by the addition of inhibitors of aromatase, a key enzyme in the biosynthesis of estradiol 17beta, or addition of tamoxifen - an estrogen receptor antagonist, to human placental minces resulted in an increase in the level of LDL-receptor mRNA. This increase could be blocked b...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Shanker YG,Shetty UP,Rao AJ

    更新日期:1998-10-01 00:00:00

  • Increased coronary arteriolar contraction to serotonin in juvenile pigs with metabolic syndrome.

    abstract::Metabolic syndrome (MetS) is associated with alterations in coronary vascular smooth muscle and endothelial function. The current study examined the contractile response of the isolated coronary arterioles to serotonin in pigs with and without MetS and investigated the signaling pathways responsible for serotonin-indu...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Lawandy I,Liu Y,Shi G,Zhang Z,Scrimgeour LA,Pavlov V,Jaworski R,Sellke FW,Feng J

    更新日期:2019-11-01 00:00:00

  • Inhibitor of growth 1 (ING1) acts at early steps of multiple DNA repair pathways.

    abstract::ING proteins are tumor suppressors involved in the regulation of gene transcription, cell cycle arrest, apoptosis, and senescence. Here, we show that ING1b expression is upregulated by several DNA-damaging agents, in a p53-independent manner. ING1b stimulates DNA repair of a variety of DNA lesions requiring activation...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Ceruti JM,Ogara MF,Menéndez C,Palmero I,Cánepa ET

    更新日期:2013-06-01 00:00:00

  • Variables of myocardial backdiffusion, determined with 17-iodo-131 heptadecanoic acid in the normal dog heart.

    abstract::Under normal and ischemic conditions backdiffusion of radiolabeled non-esterified fatty acids (NEFA) has been demonstrated. In the fasted normal canine heart the extraction fraction (EF) during interventions with glucose or lactate loading, vasodilation, and metabolic level augmentation was determined, and compared wi...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Duwel CM,Visser FC,van Eenige MJ,Roos JP

    更新日期:1989-06-27 00:00:00

  • The in vitro retardation of porcine cataractogenesis by the calpain inhibitor, SJA6017.

    abstract::Calpain inhibitors show the potential to serve as non-surgical alternatives in treating diabetic cataract and other types of these disorders. Here, we have tested the recently developed calpain inhibitor, SJA6017, for its ability to inhibit cataractogenesis in porcine lenses. These lenses were incubated in increasing ...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Biswas S,Harris F,Singh J,Phoenix DA

    更新日期:2004-06-01 00:00:00

  • Effects of streptozotocin-induced long-term diabetes on parietal cell function and morphology in rats.

    abstract::Gastric pathology is a common complication in diabetes mellitus. The aim of the study was to evaluate the functions and morphological changes of the parietal cells of the rat stomach after streptozotocin-induced diabetes. Diabetes mellitus was induced in Wistar rats by a single intraperitoneal injection of streptozoto...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Bastaki SM,Adeghate E,Chandranath IS,Amir N,Tariq S,Hameed RS,Adem A

    更新日期:2010-08-01 00:00:00

  • Expression of constitutive heat shock protein-70 in normal (non-stressed) rabbit urinary bladder tissue.

    abstract::The expression of constitutive HSP-70 in the urinary bladder was determined by SDS-PAGE and western blotting using a mouse monoclonal antibody against HSP-70. The western blot analysis showed that the mouse anti-HSP-70 cross-reacted with a 70 kDa protein present in the extracts of the urinary bladder muscle and mucosa...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Zhao Y,Levin RM,Monson FC,Chacko S

    更新日期:1993-04-07 00:00:00

  • Mitochondrial bioenergetics, uncoupling protein-2 activity, and reactive oxygen species production in the small intestine of a TNBS-induced colitis rat model.

    abstract::Inflammatory bowel disease (IBD) is often associated with a decrease in energy-dependent nutrient uptake across the jejunum that serves as the main site for absorption in the small intestine. This association has prompted us to investigate the bioenergetics underlying the alterations in jejunal absorption in 2,4,6-tri...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Al Ojaimi Y,Khachab M,Bazzi S,Bahr GM,Echtay KS

    更新日期:2020-07-01 00:00:00

  • A novel protein accumulated during maturation of the pods of the plant Impatiens balsamina.

    abstract::A high molecular weight protein has been isolated as a major polypeptide comprising 85% of the total extractable proteins in the fruit pericarp of the plant Impatiens balsamina. The protein has been purified to homogeneity following fractionation of the crude cell supernatant with ammonium sulphate, chromatography on ...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Pal M,Biswas S

    更新日期:1994-01-26 00:00:00

  • SAMD9 is a (epi-) genetically regulated anti-inflammatory factor activated in RA patients.

    abstract::To identify PBMC-expressed genes significant for RA, and to ascertain their upstream regulatory factors, as well as downstream functional effects relevant to RA pathogenesis. We performed peripheral blood mononuclear cells (PBMCs) transcriptome-wide mRNA expression profiling in a case-control discovery sample. Differe...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: He P,Wu LF,Bing PF,Xia W,Wang L,Xie FF,Lu X,Lei SF,Deng FY

    更新日期:2019-06-01 00:00:00

  • In vitro cellular uptake and neuroprotective efficacy of poly-arginine-18 (R18) and poly-ornithine-18 (O18) peptides: critical role of arginine guanidinium head groups for neuroprotection.

    abstract::We have previously demonstrated that Cationic Arginine-Rich Peptides (CARPs) and in particular poly-arginine-18 (R18; 18-mer of arginine) exhibit potent neuroprotective properties in both in vitro and in vivo neuronal injury models. Based on the current literature, there is a consensus that arginine residues by virtue...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: MacDougall G,Anderton RS,Ouliel E,Gao J,Redmond SL,Knuckey NW,Meloni BP

    更新日期:2020-01-01 00:00:00

  • PTPN12 controls PTEN and the AKT signalling to FAK and HER2 in migrating ovarian cancer cells.

    abstract::Several tyrosine phosphatases control cell motility; understanding their signaling helps to decipher cancer mechanisms. Previously, we found that the negative regulation of migration exerted by PTPN12 in ovarian cancer SKOV-3 cells involves direct FAK Y397 targeting, in HER2-dependent way. In this study, we describe t...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Villa-Moruzzi E

    更新日期:2013-03-01 00:00:00

  • Enzyme stabilization: state of the art.

    abstract::'Enzyme stabilization' is one of the most important fields in basic and applied enzymology. In basic enzymology, it is of particular relevance to understand enzyme stabilization principles first elucidating how and why the enzymes lose their biological activity and then deriving structure-stability relationships exist...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章,评审


    authors: Gianfreda L,Scarfi MR

    更新日期:1991-02-02 00:00:00

  • The effects of MAPK inhibitors on antimycin A-treated Calu-6 lung cancer cells in relation to cell growth, reactive oxygen species, and glutathione.

    abstract::Antimycin A (AMA) inhibits succinate oxidase, NADH oxidase, and mitochondrial electron transport chain between cytochrome b and c. We recently demonstrated that AMA inhibited the growth of Calu-6 lung cancer cells through apoptosis. Here, we investigated the effects of AMA and/or MAPK inhibitors on Calu-6 lung cancer ...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Han YH,Park WH

    更新日期:2010-01-01 00:00:00

  • The role of iNOS-derived NO in the antihypertrophic actions of B-type natriuretic peptide in neonatal rat cardiomyocytes.

    abstract::In the infarcted rat heart, the increase of NO occurs in the hypertrophied myocardium of non-infarcted areas and its antihypertrophic efficacy has been well established. As another endogenous regulator and the reliable index of heart pathology, B-type natriuretic peptide also exhibits the antihypertrophic properties i...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Wang T,Yan M,Li J,Zheng X

    更新日期:2007-08-01 00:00:00

  • miR-133a mediates the hypoxia-induced apoptosis by inhibiting TAGLN2 expression in cardiac myocytes.

    abstract::Myocardial hypoxia is a major cause of cardiac dysfunction due to its triggering cell injury and apoptosis. Deregulated microRNAs and their roles in cardiomyocyte apoptosis have attracted much attention. miR-133a is among the most abundant of the miRNAs present in the normal heart, and significant changes in expressio...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Li AY,Yang Q,Yang K

    更新日期:2015-02-01 00:00:00

  • IL-17A/IL-17RA promotes invasion and activates MMP-2 and MMP-9 expression via p38 MAPK signaling pathway in non-small cell lung cancer.

    abstract::The present study is to investigate the effect and mechanism of action of interleukin (IL)-17A and its receptor IL-17RA on non-small cell lung cancer (NSCLC). A total of 139 NSCLC patients were included in the study. NSCLC tissues and tumor-adjacent tissues were collected from the patients. Human NSCLC cell lines H157...

    journal_title:Molecular and cellular biochemistry

    pub_type: 临床试验,杂志文章


    authors: Wu Z,He D,Zhao S,Wang H

    更新日期:2019-05-01 00:00:00

  • Glioma: what is the role of c-Myc, hsp90 and telomerase?

    abstract::The continuous advancements in cancer research have contributed to the overwhelming evidence of the presence of telomerase in primary and secondary tumours together with hsp90 and c-Myc. This review will discuss the important role of telomerase together with hsp90 and c-Myc within the initiation and progression of gli...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章,评审


    authors: Shervington A,Cruickshanks N,Wright H,Atkinson-Dell R,Lea R,Roberts G,Shervington L

    更新日期:2006-02-01 00:00:00

  • Notch2 signaling contributes to cell growth, invasion, and migration in salivary adenoid cystic carcinoma.

    abstract::Many studies have explored whether the Notch signaling pathway has a tumor-suppressive or an oncogenic role in various tumors; however, the role of the Notch signaling pathway in salivary adenoid cystic carcinoma (SACC) is still unknown. In this study, we attempt to define the role of Notch2 signaling in cell growth, ...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Qu J,Song M,Xie J,Huang XY,Hu XM,Gan RH,Zhao Y,Lin LS,Chen J,Lin X,Zheng DL,Lu YG

    更新日期:2016-01-01 00:00:00

  • Biochemical and morphological alterations in lungs induced by experimental inhibition of fibrinolytic activity.

    abstract::The fibrinolytic system is known to play an important role in the protection of lung architecture and function. This study investigated the effects on lungs of inhibiting the fibrinolytic system using tranexamic acid (TXA). Thirty cats were used, 15 experimental and 15 control. TXA was administered intravenously to th...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Hoşgör I,Yarat A,Tüzüner N,Alkan F,Emekli N,Ahmad S

    更新日期:2002-12-01 00:00:00

  • Cytotoxicity of glucoevatromonoside alone and in combination with chemotherapy drugs and their effects on Na+,K+-ATPase and ion channels on lung cancer cells.

    abstract::Cardiac glycosides (CGs) are useful drugs to treat cardiac illnesses and have potent cytotoxic and anticancer effects in cultured cells and animal models. Their receptor is the Na+,K+ ATPase, but other plasma membrane proteins might bind CGs as well. Herein, we evaluated the short- and long-lasting cytotoxic effects o...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Schneider NFZ,Menegaz D,Dagostin ALA,Persich L,Rocha SC,Ramos ACP,Cortes VF,Fontes CFL,de Pádua RM,Munkert J,Kreis W,Braga FC,Barbosa LA,Silva FRMB,Simões CMO

    更新日期:2021-01-18 00:00:00

  • Serum concentrations of MCP-1 and IL-6 in combination predict the presence of coronary artery disease and mortality in subjects undergoing coronary angiography.

    abstract::Cytokines play a key role in the pathogenesis of coronary artery disease (CAD). The aim of current study was to investigate the relationship between the serum concentrations of 12 cytokines with mortality and extent of CAD in individuals undergoing angiography and healthy controls. 342 CAD patients were recruited and ...

    journal_title:Molecular and cellular biochemistry

    pub_type: 临床试验,杂志文章


    authors: Tajfard M,Latiff LA,Rahimi HR,Moohebati M,Hasanzadeh M,Emrani AS,Esmaeily H,Taghipour A,Mirhafez SR,Ferns GA,Mardan-Nik M,Mohammadzadeh E,Avan A,Hanachi P,Ghayour-Mobarhan M

    更新日期:2017-11-01 00:00:00

  • Metabolic studies on rabbit bladder smooth muscle and mucosa.

    abstract::Recent studies indicate that the mucosa of the urinary bladder may play a major role in the maintenance of normal bladder function. The mucosal surface of the urinary bladder serves as a protective layer against the irritative solutes found in the urine. The integrity of this barrier can be broken by overdistension, a...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Hypolite JA,Longhurst PA,Gong C,Briscoe J,Wein AJ,Levin RM

    更新日期:1993-08-11 00:00:00

  • Redox status in acute ischemic stroke: correlation with clinical outcome.

    abstract::Connection between oxidative stress and clinical outcome in acute ischemic stroke (AIS) has been poorly investigated. This study was aimed to assess redox state (through measurement of oxidative stress markers) of patients with acute ischemic stroke during different stages of follow-up period, and to find association ...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Paspalj D,Nikic P,Savic M,Djuric D,Simanic I,Zivkovic V,Jeremic N,Srejovic I,Jakovljevic V

    更新日期:2015-08-01 00:00:00

  • Expression of the ErbB4 receptor causes reversal regulation of PP2A in the Shc signal transduction pathway in human cancer cells.

    abstract::Expression of ErbB4 receptor is correlated with the incidence of non-metastatic types of human cancers, whereas the overexpression of other ErbB receptor families (ErbB1/EGFR, ErbB2 and ErbB3) is correlated to the formation of metastatic tumors. However, the molecular mechanism underlying this phenomenon has been uncl...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Yumoto N,Yu X,Hatakeyama M

    更新日期:2006-04-01 00:00:00