Abstract：
:A major component of the soluble fraction of rat heart is a homopolymer (Mr about 400-650 k) of a small protein (Mr about 20 k). This cardiac protein, which is highly homologous to alpha-B-crystallin, was isolated in its native state and visualized by electron microscopy. A homogeneous population of globular particles with an average diameter of about 14-16 nM could be seen using either negative staining or rotary shadowing procedures. The structures were globular in nature with a central depression (torus-like structures). Polyclonal antibodies, raised against the cardiac crystallin, were used for the immunocytochemical localization of the macromolecular complexes. Using fluorescent secondary antibodies, a clear and sharp striation of fixed and permeabilized rat heart myocytes could be observed, similar to that observed with anti-desmin antibodies and characteristic for the central region of the I-band. Cardiac crystallin was not found associated with F-actin in preparations of rat heart myofibrils. On the other hand, it was a major contaminant of cardiac desmin preparations. These observations suggest that cardiac crystallin is involved in the organization of cytoskeletal filaments of the Z-lines.
journal_name
Mol Cell Biochemjournal_title
Molecular and cellular biochemistryauthors
Longoni S,Lattonen S,Bullock G,Chiesi Mdoi
10.1007/BF00221053subject
Has Abstractpub_date
1990-09-21 00:00:00pages
121-8issue
2eissn
0300-8177issn
1573-4919journal_volume
97pub_type
杂志文章abstract：:Platelets are involved in hemostasis, wound healing, and tumor growth. Autologous blood products are commonly used to facilitate healing in a variety of clinical surgery applications. Recently, it was shown that platelet-rich plasma (PRP) has more specific growth factors that participate in the healing process. This s...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
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更新日期：1983-01-01 00:00:00
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更新日期：2013-01-01 00:00:00
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更新日期：2013-12-01 00:00:00
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更新日期：2015-01-01 00:00:00
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章,评审
doi：10.1007/BF00234161
更新日期：1991-02-02 00:00:00
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/BF00944200
更新日期：1994-10-12 00:00:00
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journal_title：Molecular and cellular biochemistry
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doi：
更新日期：2002-10-01 00:00:00
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journal_title：Molecular and cellular biochemistry
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更新日期：1997-05-01 00:00:00
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更新日期：1991-02-27 00:00:00
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journal_title：Molecular and cellular biochemistry
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更新日期：2002-05-01 00:00:00
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更新日期：2010-11-01 00:00:00
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journal_title：Molecular and cellular biochemistry
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更新日期：1995-03-09 00:00:00
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更新日期：2008-09-01 00:00:00
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章,评审
doi：10.1023/a:1012261903611
更新日期：2001-09-01 00:00:00
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章,评审
doi：
更新日期：1998-03-01 00:00:00
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journal_title：Molecular and cellular biochemistry
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更新日期：1993-08-11 00:00:00
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pub_type： 杂志文章
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更新日期：2019-04-01 00:00:00
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章,评审
doi：
更新日期：1998-11-01 00:00:00
