当前位置： SCI文献检索 > MOLECULAR AND CELLULAR BIOCHEMISTRY期刊下所有文献 > Evidence that glycine induces lipid peroxidation and decreases glutathione concentrations in rat cerebellum.

Evidence that glycine induces lipid peroxidation and decreases glutathione concentrations in rat cerebellum.

Abstract：

:Patients with non-ketotic hyperglycinemia (NKH) present severe neurological symptoms and brain abnormalities involving cerebellum. Although the pathomechanisms underlying the cerebellum damage have not been studied, high tissue levels of glycine (GLY), the biochemical hallmark of this disorder have been suggested to contribute to the neuropathology of this disease. We investigated the in vitro effects of GLY on important parameters of oxidative stress and energy metabolism in cerebellum of 30-day-old rats. Our results show that GLY increased 2',7'-dichlorofluorescin oxidation, suggesting that reactive species production are augmented by GLY in the cerebellum. However, hydrogen peroxide generation was not altered by GLY. GLY also increased thiobarbituric acid-reactive substances (TBA-RS) levels and reduced the glutathione (GSH) content, indicating that this amino acid provokes lipid oxidative damage and compromises the non-enzymatic antioxidant defenses, respectively, in cerebellum. The antioxidants melatonin and trolox (the hydrosoluble analog of vitamin E) prevented the GLY-induced increase of TBA-RS and decrease of GSH in cerebellum, indicating the involvement of hydroxyl and peroxyl radicals in these effects. The NMDA receptor antagonist MK-801 also attenuated GLY-induced decrease of GSH, suggesting that this effect is mediated through NMDA receptor. In contrast, GLY did not alter the protein carbonyl formation and total and protein-bound sulfhydryl group content, as well as catalase and superoxide dismutase activities. Furthermore, GLY did not alter the activities of the respiratory chain complexes and creatine kinase. Our present data indicate that oxidative stress elicited by GLY in vitro may be a potential pathomechanism involved in the cerebellar dysfunction observed in NKH.

journal_name

Mol Cell Biochem

journal_title

Molecular and cellular biochemistry

authors

Moura AP,Grings M,Marcowich GF,Bumbel AP,Parmeggiani B,de Moura Alvorcem L,Wajner M,Leipnitz G

doi

10.1007/s11010-014-2118-z

subject

Has Abstract

pub_date

2014-10-01 00:00:00

pages

125-34

issue

1-2

eissn

0300-8177

issn

1573-4919

journal_volume

395

pub_type

杂志文章

在线工具

Evidence that glycine induces lipid peroxidation and decreases glutathione concentrations in rat cerebellum.