Abstract:
:Studies show that genetic polymorphisms in apolipoproteins, which are in charge of lipid transport, predispose to atherogenic dyslipidemia. This study aimed to investigate the impact of apolipoprotein E, A5, and B genotypes and dietary intake on lipid profile in a sample of elderly women in Brazil. Two hundred and fifty-two women (60 years or older) living in the outskirts of the Brazilian Federal District underwent clinical and laboratory assessments to characterize glycemic and lipidemic variables, and also to exclude confounding factors (smoking, drinking, hormone replacement, cognitive impairment, physical activity). Three-day food records were used to determine usual dietary intake, whereas genotypic evaluations were in accordance to established methodologies. Genotype frequencies were consistent with the Hardy-Weinberg equilibrium. Prior to adjustment, individuals carrying the epsilon2 allele showed higher serum levels of triglycerides (P<0.05) and VLDL (P<0.005) compared to epsilon4 carriers, whereas LDL levels were considerably elevated in epsilon4 compared to epsilon2 carriers. In the presence of high intake of total fat or a low ratio of polyunsaturated to saturated fatty acid, epsilon4 carriers lost protection against hypertriglyceridemia. There was no association of the apolipoprotein A5 and B genotypes with lipidemic levels independently of the fat intake regimen. Results are suggestive of a dysbetalipoproteinemic-like phenotype in postmenopausal women, with remarkable gene-diet interaction.
journal_name
Mol Cell Biochemjournal_title
Molecular and cellular biochemistryauthors
Paula RS,Souza VC,Benedet AL,Souza ER,Toledo JO,Moraes CF,Gomes L,Alho CS,Córdova C,Nóbrega OTdoi
10.1007/s11010-009-0313-0subject
Has Abstractpub_date
2010-04-01 00:00:00pages
307-15issue
1-2eissn
0300-8177issn
1573-4919journal_volume
337pub_type
杂志文章abstract::A method is described for analysing molecular species of glycerophospholipids. Diglycerides obtained by phospholipase C-catalysed hydrolysis of the phospholipid are separated into the diacyl- alkylacyl- and alkenylacyl- subclasses by HPLC on silicic acid. The molecular species of diacylglycerol are separated by HPLC o...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:
更新日期:1997-07-01 00:00:00
abstract::S-glutathionylation, the formation of mixed disulfides of glutathione with cysteine residues of proteins, is a broadly observed physiological modification that occurs in response to oxidative stress. Since cysteine residues are particularly susceptible to oxidative modification by reactive oxygen species, S-glutathion...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/s11010-007-9501-y
更新日期:2007-10-01 00:00:00
abstract::This study was undertaken to clarify the role of high-energy phosphate metabolism in hydrogen peroxide-induced cardiac dysfunction using phosphorus and fluorine nuclear magnetic resonance spectroscopy. The exposure of a Langendorff-perfused heart to hydrogen peroxide (200-400 micromol/L, 8 min) provoked biphasic contr...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1023/a:1007042611127
更新日期:2000-01-01 00:00:00
abstract::The phosphorylation of rat cardiac microsomal proteins was investigated with special attention to the effects of okadaic acid (an inhibitor of protein phosphatases), inhibitor 2 of protein phosphatase 1 and inhibitor of cyclic AMP-dependent protein kinase (protein kinase A). The results showed that okadaic acid (5 mic...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1023/a:1006879427457
更新日期:1997-10-01 00:00:00
abstract::Endothelial progenitor cell (EPC) transplantation has shown advantages in the treatment of myocardial infarction (MI) in animal models and clinical trials through mechanisms of direct intercellular contacts, autocrine, and paracrine. However, the effects of EPC transplantation for MI treatment remain controversial and...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/s11010-020-03945-x
更新日期:2020-10-23 00:00:00
abstract::Sensitive to Apoptosis Gene (SAG), a RING component of SCF E3 ubiquitin ligase, was shown to be phosphorylated by protein kinase CK2 at the Thr10 residue. It is, however, unknown whether this phosphorylation is stress-responsive or whether the phosphorylation changes its E3 ubiquitin ligase activity. To address these,...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/s11010-006-9287-3
更新日期:2007-01-01 00:00:00
abstract::Liver-X-Receptor alpha (LXR-alpha) that belongs to nuclear receptor/transcriptional factor family has been recognized to play crucial role in the regulation of lipid metabolism and inflammation. Consequently, the present study was addressed to explore the functional genomics of LXR-alpha within human blood immunomodul...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/s11010-006-9216-5
更新日期:2006-11-01 00:00:00
abstract::Three antisera were prepared against non-histone protein classes named NHCP1, NHCP2 and dehistonized chromatin (with different affinity to DNA) from hamster liver. Two main antigenic bands of MW 17,000 and 36,000 were specific in the NHCP1 fraction and one antigen of MW 56,000 was specific for the NHCP2 fraction from ...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/BF00220720
更新日期:1990-01-18 00:00:00
abstract::The pyridine nucleotides have important non-redox activities as cellular effectors and metabolic regulators [1-3]. The enzyme-catalyzed cleavage of the nicotinamide-ribosyl bond of NAD+ and the attendant delivery of the ADPRibosyl moiety to acceptors is central to these many diverse biological activities. Included are...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章,评审
doi:10.1007/BF00928468
更新日期:1994-09-01 00:00:00
abstract::Vascular endothelial and -smooth muscle cells have been shown to use fatty acids as substrates for oxidative phosphorylation. Endothelial cells are more vulnerable to oxidative stress than muscle cells and are prone to loose carnitine early during hypoperfusion. This has been suggested by two observations. The first i...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/BF01270579
更新日期:1992-10-21 00:00:00
abstract::Previous studies reported that estrogen receptor β (ERβ) is localized to mitochondria, whereas little is known about the physiological functions of mitochondrial ERβ. In the present study, we explored the role of mitochondrial ERβ in regulating apoptosis using stable ERβ-expressing and ERβ knockdown cells lines. We fo...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/s11010-014-2293-y
更新日期:2015-03-01 00:00:00
abstract::Recent studies have shown that adult osteoporosis could be induced by ageing and estrogen deficiency and homocysteine (Hcy) is an independent risk factor of fracture in osteoporosis patients. In this study, we found hypermethylation of the promoter A region in the estrogen receptor alpha (ERα) gene, and methylation in...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/s11010-011-0836-z
更新日期:2011-09-01 00:00:00
abstract::Fish and fish oils contain the omega-3 fatty acids known as eicosapentaenoic acid (EPA) plus docosahexaenoic acid (DHA). Epidemiological studies have shown an inverse relation between the dietary consumption of fish containing EPA/DHA and mortality from coronary heart disease. These relationships have been substantiat...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1023/B:MCBI.0000041863.11248.8d
更新日期:2004-08-01 00:00:00
abstract::Synthetic peptides reproducing the amino and carboxyl terminal region of CK2beta subunit have been analyzed for their ability to mimic different properties of full length beta subunit. Peptide beta[1-77], containing both the autophosphorylation site and the down-regulatory domain 55-64, is readily phosphorylated by al...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:
更新日期:1999-01-01 00:00:00
abstract::Colchicine has been demonstrated to suppress the release of fibroblast growth factors, retard collagen formation and augment collagenase activity. Trials with colchicine in patients with hepatic fibrosis have suggested clinical benefit. The development of impaired myocardial function in the spontaneously hypertensive ...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1023/a:1006889126666
更新日期:1997-01-01 00:00:00
abstract::The present study was aimed to investigate the protective effects of 17β-estradiol (E2) and estrogen receptor α (ERα) on isoproterenol (ISO)-treated H9c2 cardiomyoblast cells. In the present study, we treated H9c2 cells with ISO, a β-adrenergic receptor agonist, to induce myocardiac hypertrophy. Pre-administration of ...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/s11010-017-3048-3
更新日期:2017-10-01 00:00:00
abstract::Alpha-fetoprotein (AFP) for long was known as immunomodulator and tumor marker having multifaceted actions on the activity of normal and transformed cells. In present study, we have investigated the involvement of AFP in regulation of THP-1 cell line invasion and underlying mechanisms. Treatment with human recombinant...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/s11010-013-1650-6
更新日期:2013-07-01 00:00:00
abstract::Glucocorticoids inhibit transcription of the genes encoding rRNA (rDNA) in P1798 lymphoma cells. This is due to a decrease in the amount or activity of a transcription factor, called TFIC. TFIC has been purified to apparent homogeneity and the properties of this protein have been investigated in detail. TFIC is tightl...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/BF00229820
更新日期:1991-05-29 00:00:00
abstract::There is a wealth of information regarding the import and export of nuclear proteins in general. Nevertheless, the available data that deals with the nucleocytoplasmic movement of steroid hormone receptors remains highly limited. Some research findings reported during the past five years have succeeded in identifying ...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章,评审
doi:10.1023/b:mcbi.0000026060.81570.35
更新日期:2004-05-01 00:00:00
abstract::Stress genes can be ascribed to have been generated by the organism for their intrinsic urge to survive against the changing environmental odds, during the evolutionary process. This concept has been supported by a large number of reports describing individual types of phenomena. These have been reconciled and globali...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章,评审
doi:
更新日期:1999-06-01 00:00:00
abstract::Cigarette smoking is associated with a number of fatal diseases, including cancer of different organs. A number of oxoaldehydes are found in cigarette smoke, among which methylglyoxal (MG) is known to cause toxicity to cells upon accumulation. In biological systems, MG is converted to s-d-lactoylglutathione by glyoxal...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1023/a:1017992403656
更新日期:2002-01-01 00:00:00
abstract::Ciprofloxacin induced an increment of reactive oxygen species in sensitive strains of Staphylococcus aureus leading to oxidative stress detected by chemiluminescence while resistant strains did not suffer such stress. Oxidation of lipids was performed by employing thiobarbituric acid reaction to detect the formation o...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/s11010-005-9051-0
更新日期:2006-04-01 00:00:00
abstract::Connection between oxidative stress and clinical outcome in acute ischemic stroke (AIS) has been poorly investigated. This study was aimed to assess redox state (through measurement of oxidative stress markers) of patients with acute ischemic stroke during different stages of follow-up period, and to find association ...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/s11010-015-2425-z
更新日期:2015-08-01 00:00:00
abstract::The epithelial cells of the human intestine exhibit a cholinesterase activity which is restricted to the apex of the villi. This activity displays a maximum in the colon and a minimum in the jejunum. Contrary to most of the studied vertebrates, the human cells present both acetylcholinesterase and butyrylcholinesteras...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/BF00233119
更新日期:1991-12-11 00:00:00
abstract::Adriamycin (doxorubicin) is one of the most effective chemotherapeutic agents against a variety of cancers, but its usefulness is seriously curtailed by the risk of developing heart failure. Available laboratory evidence suggests that an increase in oxidative stress, brought about by increased free radical production ...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章,评审
doi:10.1023/a:1007094214460
更新日期:2000-04-01 00:00:00
abstract::Histone acetylation and deacetylaion play important roles in chromatin remodeling and gene expression. An imbalance of these reactions leads to aberrant behavior of the cells in the cell cycle, which in turn contributes to carcinogenesis. Histone deacetylase (HDAC) inhibitors have been shown to have anti-tumor effects...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/s11010-010-0510-x
更新日期:2010-10-01 00:00:00
abstract::A membrane-bound L-asparaginase (EC 3.5.1.1) of Tetrahymena pyriformis was purified to homogeneity. The purified enzyme is a lipoprotein, since it is inactivated by phospholipase C and its activity is restored by the addition of naturally occurring lipids, such as phosphatidylcholine, triolein and oleyl acetate. The r...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/BF00226142
更新日期:1988-10-01 00:00:00
abstract::Apoptosis is a physiological mechanism for the control of DNA integrity in mammalian cells. Vanadium induces both DNA damage and apoptosis. It is suggested that vanadium-induced apoptosis serves to eliminate DNA-damaged cells. This study is designed to clarify a role of reactive oxygen species in the mechanism of apop...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1023/a:1007078915585
更新日期:1999-12-01 00:00:00
abstract::Ischemia/reperfusion injury causes cardiomyocyte apoptosis, ventricular remodeling, leading to a dilated heart. Hypoxia is one of the causes involved in ischemia damage, and BNIP3 is a hypoxia-inducible marker and also a sensor to induce mitochondria-dependent apoptosis. Recent reports discussed ablating BNIP3 can res...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/s11010-010-0578-3
更新日期:2010-12-01 00:00:00
abstract::It has been reported that apelin functions as an adipokine, which has been associated to obesity and insulin resistance. The objective of this study was to analyze the apelin mRNA expression in white adipose tissue (WAT) from high-fat (Cafeteria) fed rats, in order to examine potential relationships with obesity marke...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/s11010-007-9531-5
更新日期:2007-11-01 00:00:00