Abstract：
:delta-Crystallin is a major structural protein of avian and reptilian lenses that is absent from the lenses of fish, amphibia and mammals. It appears to be a tetrameric protein with a native molecular weight near 200 000 (200K) and polypeptide molecular weight near 50K and 48K) (see Note added in proof). The alpha-crystallin polypeptides are extremely similar, associate in various combinations of four and are held together by hydrophobic interactions. Although principally cytoplasmic, delta-crystallin may associate with the cell membrane. delta-Crystallin differs from other lens crystallins in its alpha-helical content, native and subunit molecular weights, antigenicity, low wavelength of maximum fluorescence emission (315 nm) after excitation at 280 nm and amino acid composition (high in leucine; low in aromatic residues en no cysteine). Analyses of peptides, native and subunit molecular weights, and circular dichroism spectra indicate that the primary, secondary, tertiary and subunit structures of delta-crystallin have been generally conserved during evolution. There are at least two tandemly arranged delta-crystallin containing 13-15 introns in the chicken; a similar structure exists for a cloned delta-crystallin gene in the duck. Experiments with chicken show that delta-crystallin synthesis occurs principally in the embryo, especially during lens fiber cell differentiation. delta-Crystallin synthesis also takes place during lens fiber cell differentiation in culture. There is evidence for both transcriptional and post-transcriptional regulation of delta-crystallin synthesis. Current studies on the crystallographic and primary structures of delta-crystallin, on the structure, evolution and expression of the delta-crystallin genes, and on the translation of delta-crystallin mRNAs make this specialized lens protein an active area of investigation.
journal_name
Mol Cell Biochemjournal_title
Molecular and cellular biochemistryauthors
Piatigorsky Jdoi
10.1007/BF00231304subject
Has Abstractpub_date
1984-01-01 00:00:00pages
33-56issue
1-2eissn
0300-8177issn
1573-4919journal_volume
59pub_type
杂志文章,评审abstract：:Two membrane bound guanylate cyclases are expressed in vertebrate photoreceptor cells. They serve a key function in photoreceptor physiology as they synthesize the intracellular transmitter of photoexcitation guanosine 3',5'-cyclic monophosphate (cGMP). Both cyclases named ROS-GC1 and ROS-GC2 form a subclass of membra...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章,评审
doi：
更新日期：2002-01-01 00:00:00
abstract：:Gastric pathology is a common complication in diabetes mellitus. The aim of the study was to evaluate the functions and morphological changes of the parietal cells of the rat stomach after streptozotocin-induced diabetes. Diabetes mellitus was induced in Wistar rats by a single intraperitoneal injection of streptozoto...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-010-0435-4
更新日期：2010-08-01 00:00:00
abstract：:The sarcoplasmic reticulum (SR) plays a critical role in mediating cardiac contractility and its function is abnormal in the diabetic heart. However, the mechanisms underlying SR dysfunction in the diabetic heart are not clear. Because protein phosphorylation regulates SR function, this study examined the phosphorylat...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1023/b:mcbi.0000028762.97754.26
更新日期：2004-06-01 00:00:00
abstract：:The present study investigates the prophylactic effect of Nymphaea alba against ferric nitrilotriacetate (Fe-NTA)-induced renal oxidative stress, hyperproliferative response and renal carcinogenesis in Wistar rats. Treatment with Fe-NTA (9 mg Fe/kg body weight, intraperitoneally) enhanced iron-ascorbate-induced renal ...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-005-2258-2
更新日期：2005-03-01 00:00:00
abstract：:Cultured endothelial cells have been used in the past as a source of endothelium-derived relaxing factor (EDRF) and of prostacyclin (PGI2). Although cell cultures are essential for observation of prolonged exposure to media or when there is delayed response, they are time consuming and sterile conditions are essential...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/BF00239544
更新日期：1991-11-13 00:00:00
abstract：:Carbohydrate has been removed from a number of glycoproteins without major effect on the structure or enzyme activity of the protein. Thus carbohydrate has been suggested to underly a non-primary function for proteins, such as in relatively non-specific interactions with other carbohydrates or macromolecules, stabiliz...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章,评审
doi：10.1007/BF00230632
更新日期：1986-11-01 00:00:00
abstract：:Natural compounds with free-radical scavenging activity have potential role in maintaining human health and preventing diseases. In this study, we report the antioxidant and cytoprotective properties of 14-aminotetradecanoic acid (ATDA) isolated from the Decalepis hamiltonii roots. ATDA is a potent scavenger of supero...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-011-1196-4
更新日期：2012-05-01 00:00:00
abstract：:Many recent studies have suggested that bergapten (BP), a class of native compound with numerous biological activities such as anti-resorptive properties, may exert protective effects against postmenopausal bone loss. However, it remains unknown whether BP regulates or improves the osteogenic function of bone marrow s...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-015-2517-9
更新日期：2015-11-01 00:00:00
abstract：:The levels of plasma apolipoprotein (apo) E, an anti-atherogenic protein involved in mammalian cholesterol transport, were found to be 2-3 fold lower in mice over-expressing human apoA-I gene. ApoE is mainly associated with VLDL and HDL-size particles, but in mice the majority of the apoE is associated with the HDL pa...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1023/a:1007107712725
更新日期：2000-06-01 00:00:00
abstract：:The objective of the present study was to compare energy substrate fluxes through metabolic pathways leading to mitochondrial citrate synthesis and release in normal and diseased rat hearts using 13C-substrates and mass isotopomer analysis by gas chromatography-mass spectrometry (GCMS). This study was prompted by our ...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：
更新日期：2003-01-01 00:00:00
abstract：:Previously, we isolated and characterized the gene encoding the 3'-Nucleotidase/Nuclease (Ld3'NT/NU) from the human pathogen, Leishmania donovani. This unique cell surface enzyme has been shown to be involved in the salvage of host-derived purines, which are essential for the survival of this important protozoan paras...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1023/a:1010809420104
更新日期：2001-04-01 00:00:00
abstract：:The inhibitory effect of menaquinone-7 (MK-7; vitamin K2) on osteoclast-like cell formation and osteoclastic bone resorption in vitro is found (Mol Cell Biochem 228: 39-47, 2001). This study, furthermore, was undertaken to determine the effect of MK-7 on the bone-resorbing factor-induced bone resorption using the femo...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1023/a:1022818111655
更新日期：2003-03-01 00:00:00
abstract：:Molecular hydrogen (H2) is an agent with potential applications in oxidative stress-related and/or inflammatory disorders. H2 is usually administered by inhaling H2-containing air (HCA) or by oral intake of H2-rich water (HRW). Despite mounting evidence, the molecular mechanism underlying the therapeutic effects and t...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-015-2353-y
更新日期：2015-05-01 00:00:00
abstract：:Arjunolic acid, a new triterpene and a potent principle from the bark of Terminalia arjuna, has been shown to provide significant cardiac protection in isoproterenol induced myocardial necrosis in rats. To further explore the mechanism of action of arjunolic acid, antiplatelet activity, anticoagulant assays, electroca...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1023/a:1011927812753
更新日期：2001-08-01 00:00:00
abstract：:It has been reported that apelin functions as an adipokine, which has been associated to obesity and insulin resistance. The objective of this study was to analyze the apelin mRNA expression in white adipose tissue (WAT) from high-fat (Cafeteria) fed rats, in order to examine potential relationships with obesity marke...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-007-9531-5
更新日期：2007-11-01 00:00:00
abstract：:Cyclic AMP (300 micron) activates phosphofructokinase from dialyzed haemolysates of mature rat erythrocytes. The main conclusions are: a) Cyclic AMP, at pH 7.1 and low concentrations of fructose-6-phosphate, is able to reverse the inhibition produced by different amounts of ATP (up to 1.5 mM). b) The cyclic nucleotide...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/BF01734111
更新日期：1977-05-03 00:00:00
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-013-1848-7
更新日期：2014-01-01 00:00:00
abstract：:The present study is to investigate the effect and mechanism of action of interleukin (IL)-17A and its receptor IL-17RA on non-small cell lung cancer (NSCLC). A total of 139 NSCLC patients were included in the study. NSCLC tissues and tumor-adjacent tissues were collected from the patients. Human NSCLC cell lines H157...
journal_title：Molecular and cellular biochemistry
pub_type： 临床试验,杂志文章
doi：10.1007/s11010-018-3483-9
更新日期：2019-05-01 00:00:00
abstract：:The results of our previous studies demonstrated that acute streptozotocin-induced diabetes in rats evoked a decrease in skin collagen content with little effect on glycosaminoglycans (GAG) content. In our present study we employed the model of chronic diabetes in order to check its effect on skin GAG content. It was ...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/BF00248454
更新日期：1996-01-12 00:00:00
abstract：:Endothelial cell nitric oxide synthase (NOS) is known to have a N-myristoylation consensus sequence. Such a consensus sequence is not evident in the macrophage, smooth muscle and neuronal NOS. A functional role for this N-terminal myristoylation is not clear yet. In the present study, we examined the effect of N-termi...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/BF01076076
更新日期：1995-11-22 00:00:00
abstract：:6, 4'-Dihydroxy-7-methoxyflavanone (DMF) has been shown to possess anti-inflammatory, anti-oxidative, and neuroprotective activities. However, its effect on oxidative stress-induced aging remains undemonstrated. This study aimed at investigating the anti-senescence effect of DMF on hydrogen peroxide (H2O2)-induced pre...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-020-03951-z
更新日期：2020-10-27 00:00:00
abstract：:The EDA+ fibronectin splicing variant is overexpressed in psoriatic non-lesional epidermis and sensitizes keratinocytes to mitogenic signals. However, regulation of its abundance is only partially understood. In our recent cDNA microarray experiment, we identified three SR-rich splicing factors-splicing factor, argini...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-017-3090-1
更新日期：2017-12-01 00:00:00
abstract：:Tripeptide aminopeptidase (EC 3.4.11.4) was purified from bovine dental follicles by a series of chromatographies. Purified enzyme had a specific activity of 59.5 units per mg protein with L-prolyl-glycylglycine as substrate. The pH optimum was 8.0. The purified native enzyme had a Mr of 230,000 and was shown to be a ...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/BF00926579
更新日期：1993-12-08 00:00:00
abstract：UNLABELLED:Inhibition of Na+/H+ exchange with amiloride analogues has been shown to provide functional protection during ischemia and reperfusion and to reduce infarct size in isolated rat hearts. In rat hearts, treatment with ethylisopropyl-amiloride (EIPA, a selective Na+/H- exchange inhibitor) was additive to the pr...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：
更新日期：1998-09-01 00:00:00
abstract：:Oxidation of semiquinone by O2 in the Q cycle is known to be one of the sources of superoxide anion (O2.-) in aerobic cells. In this paper, such a phenomenon was analyzed using the chemical kinetics model of electron transfer from succinate to cytochrome c, including coenzyme Q, the complex III non-heme iron protein F...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：
更新日期：1998-07-01 00:00:00
abstract：:The renin-angiotensin (RAS) pathway has an important role in the etiology of heart failure and given the importance of RAS as a therapeutic target in various cardiomyopathies, genetic polymorphisms in the RAS genes may modulate the risk and severity of disease in cardiomyopathy patients. In the present study, we exami...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-016-2891-y
更新日期：2017-03-01 00:00:00
abstract：:To identify PBMC-expressed genes significant for RA, and to ascertain their upstream regulatory factors, as well as downstream functional effects relevant to RA pathogenesis. We performed peripheral blood mononuclear cells (PBMCs) transcriptome-wide mRNA expression profiling in a case-control discovery sample. Differe...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-019-03499-7
更新日期：2019-06-01 00:00:00
abstract：:The ex vivo and in vitro effects of quercetin on NTPDase, adenosine deaminase (ADA), and acetycholinesterase (AChE) activities in lymphocytes, as well as the effects of quercetin on butyrylcholinesterase (BChE) activity in serum and myeloperoxidase (MPO) activity in plasma were determined in rats. For the ex vivo expe...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-014-2155-7
更新日期：2014-11-01 00:00:00
abstract：:Previous results with p9-RNA, obtained from lymph nodes of animals immunized with the peptide p9 of HIV-1, suggested that its effects on lymphocytes could be mediated by RNA-dependent protein kinase (PKR). Here we report that p9-RNA activates PKR leading to the degradation of the inhibitor I-kappaB alpha and the conco...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1023/a:1024107512419
更新日期：2003-05-01 00:00:00
abstract：:Placental chorionic somatomammotropin (hCS-A or B) and growth hormone variant (hGH-V) are members of the human growth hormone family, and are related by structure and function to pituitary growth hormone (hGH-N). However, while the hGH-N gene is expressed specifically in the anterior pituitary, hGH-V and hCS are produ...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/BF00230184
更新日期：1991-08-14 00:00:00
