Abstract：
:Renal PEPT1 and PEPT2 cotransporters play an important role in the balance of circulating body oligopeptides and selected peptidomimetic drugs. We aim to comprehensively characterise age-related changes of the renal PEPT cotransporters at the gene, protein, and functional level. Brush border membrane vesicles (BBMV) and outer medulla membrane vesicles (OMMV) were isolated from the kidneys of young, middle-aged and old rats. The protein expression of PEPT1 was not only increased in BBMV from old rats, but PEPT1 also appeared in OMMV from middle-aged and old rats. SLC15A1 gene expression in the renal cortex increased in middle-aged group. PEPT2 protein expression was not only increased with ageing, but PEPT2 also was found in BBMV from middle-aged and old groups. SLC15A2 gene expression in the renal outer medulla increased in the old group. These changes in the expressions and localisations of PEPT1 and PEPT2 could explain the changes to transport activity in BBMV and OMMV. These findings provide novel insights that would be useful for maintaining protein nutrition and optimising the delivery of some peptidomimetic drugs in elderly individuals.
journal_name
Mol Cell Biochemjournal_title
Molecular and cellular biochemistryauthors
Alghamdi OA,King N,Andronicos NM,Jones GL,Chami B,Witting PK,Moens PDJdoi
10.1007/s11010-018-3413-xsubject
Has Abstractpub_date
2019-02-01 00:00:00pages
71-82issue
1-2eissn
0300-8177issn
1573-4919pii
10.1007/s11010-018-3413-xjournal_volume
452pub_type
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