当前位置： SCI文献检索 > MOLECULAR AND CELLULAR BIOCHEMISTRY期刊下所有文献 > Two structurally distinct and spatially compartmentalized adenylate kinases are expressed from the AK1 gene in mouse brain.

Two structurally distinct and spatially compartmentalized adenylate kinases are expressed from the AK1 gene in mouse brain.

Abstract：

:Adenylate kinases (AK, EC 2.7.4.3) have been considered important enzymes for energy homeostasis and metabolic signaling. To gain a better understanding of their cell-specific significance we studied the structural and functional aspects of products of one adenylate kinase gene, AK1, in mouse tissues. By combined computer database comparison and Northern analysis of mRNAs, we identified transcripts of 0.7 and 2.0 kilobases with different 5' and 3' non-coding regions which result from alternative use of promoters and polyadenylation sites. These mRNAs specify two distinct proteins, AK1 and a membrane-bound AK1 isoform (AK1beta), which differ in their N-terminal end and are co-expressed in several tissues with high-energy demand, including the brain. Immunohistochemical analysis of brain tissue and primary neurons and astrocytes in culture demonstrated that AK1 isoforms are expressed predominantly in neurons. AK1beta, when tested in transfected COS-1 and N2a neuroblastoma cells, located at the cellular membrane and was able to catalyze phosphorylation of ADP in vitro. In addition, AK1beta mediated AMP-induced activation of recombinant ATP-sensitive potassium channels in the presence of ATP. Thus, two structurally distinct AK1 isoforms co-exist in the mouse brain within distinct cellular locations. These enzymes may function in promoting energy homeostasis in the compartmentalized cytosol and in translating cellular energetic signals to membrane metabolic sensors.

journal_name

Mol Cell Biochem

journal_title

Molecular and cellular biochemistry

authors

Janssen E,Kuiper J,Hodgson D,Zingman LV,Alekseev AE,Terzic A,Wieringa B

doi

10.1023/b:mcbi.0000009859.15267.db

keywords:

subject

Has Abstract

pub_date

2004-01-01 00:00:00

pages

59-72

issue

1-2

eissn

0300-8177

issn

1573-4919

journal_volume

256-257

pub_type

杂志文章

相关文献

MOLECULAR AND CELLULAR BIOCHEMISTRY文献大全
  • Identification of an HMG-like protein involved in regulation of Na+/H+ exchanger expression.

    abstract：:In this study we characterized regulation of the Na+/H+ exchanger promoter in several tissue types. A conserved poly (dA:dT) region was important in regulation of the promoter. Nuclear extracts from rat myocardium and from mouse proximal tubule cells protected the poly (dA:dT) region of the NHE1 promoter. A protein fr...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：

    authors： Wang H,Yang W,Fliegel L

    更新日期：1997-11-01 00:00:00

  • Alterations of biochemical marker levels and myonuclear numbers in rat skeletal muscle after ischemia-reperfusion.

    abstract：:Prolonged ischemia-reperfusion results in various damages in skeletal muscle. Following reperfusion, although the damaged muscles undergo regeneration, the precise process and mechanism of regeneration have not yet been fully understood. Here, we show the altered levels of plasma biochemical markers of muscle damage, ...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-012-1470-0

    authors： Itoh M,Shimokawa N,Tajika Y,Murakami T,Aotsuka N,Lesmana R,Farenia R,Iwasaki T,Okda J,Yorifuji H,Koibuchi N

    更新日期：2013-01-01 00:00:00

  • p38 Mitogen-activated protein kinase affects transforming growth factor-beta/Smad signaling in human dental pulp cells.

    abstract：:Transforming Growth Factor-beta (TGF-beta) plays an essential role in differentiation of dental pulp cells into odontoblasts during reparative dentine formation. However, the mechanism by which TGF-beta stimulates dental repair remains rather obscure. Human dental pulp cells were used as an in vitro model in the prese...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-006-9193-8

    authors： Wang FM,Hu T,Tan H,Zhou XD

    更新日期：2006-10-01 00:00:00

  • Proteomics analysis of plasma membrane from liver sinusoidal endothelial cells after partial hepatectomy by an improved two-dimensional electrophoresis.

    abstract：:Liver regeneration is an angiogenesis-associated phenomenon. To identify key plasma membrane (PM) proteins of endothelial cells involved in the initiation of angiogenesis during liver regeneration, the PM of liver sinusoidal endothelial cells (LSEC) at 72 h after partial hepatectomy was enriched by an established in v...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-010-0537-z

    authors： Li X,Xiong L,Xie C,Cao J,Deng H,Lin Y,Cao R,Li J,Chen P,Liang S

    更新日期：2010-11-01 00:00:00

  • Silencing of lncRNA DLEU1 inhibits tumorigenesis of ovarian cancer via regulating miR-429/TFAP2A axis.

    abstract：:Long non-coding RNAs (lncRNAs) are known as crucial regulators in the development of OC. In the current study, we aim to explore the function and molecular mechanism of lncRNA DLEU1 in OC. Quantitative real-time polymerase chain reaction (qRT-PCR) was applied to determine the expression of DLEU1, miR-429, and TFAP2A i...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-020-03971-9

    authors： Xu H,Wang L,Jiang X

    更新日期：2020-11-10 00:00:00

  • Thyroid hormone and cardioprotection: study of p38 MAPK and JNKs during ischaemia and at reperfusion in isolated rat heart.

    abstract：:It has been recently shown that long-term thyroxine administration increases the tolerance of the heart to ischaemia. The present study investigated whether thyroxine induced cardioprotection involves alterations in the pattern of p38 mitogen activated protein kinase (p38MAPK) and c-Jun NH2-terminal kinases (JNKs) act...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：

    authors： Pantos C,Malliopoulou V,Paizis I,Moraitis P,Mourouzis I,Tzeis S,Karamanoli E,Cokkinos DD,Carageorgiou H,Varonos D,Cokkinos DV

    更新日期：2003-01-01 00:00:00

  • The effects of chronic administration of nandrolone decanoate on redox status in exercised rats.

    abstract：:For the past 40 years, anabolic-androgenic steroids have been used by a wide variety of athletes with the hope of improving their training, endurance, and performance. The aim of this study was to examine the chronic effects of nandrolone decanoate (20 mg/kg, s.c, Deca-Durabolin DECA(®)) on oxidative stress biomarkers...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-015-2571-3

    authors： Nikolic T,Zivkovic V,Jevdjevic M,Djuric M,Srejovic I,Djuric D,Jeremic N,Djuric D,Bolevich S,Jakovljevic V

    更新日期：2016-01-01 00:00:00

  • Modulation of antioxidant enzyme activities, platelet aggregation and serum prostaglandins in rats fed spray-dried milk containing n-3 fatty acid.

    abstract：:Spray-dried milk enriched with n-3 fatty acids from linseed oil (LSO) or fish oil (FO) were fed to rats to study its influence on liver lipid peroxides, hepatic antioxidant enzyme activities, serum prostaglandins and platelet aggregation. Significant level of alpha linolenic acid, eicosapentaenoic acid and docosahexae...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-005-7094-x

    authors： Ramaprasad TR,Baskaran V,Krishnakantha TP,Lokesh BR

    更新日期：2005-09-01 00:00:00

  • Features of short-term myocardial hibernation.

    abstract：:When severe ischemia, such as that resulting from a sudden and complete coronary artery occlusion, is prolonged for more than 20-40 min, myocardial infarction develops, and there is irreversible loss of contractile function. When myocardial ischemia is less severe but nevertheless prolonged, the myocardium is dysfunct...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章,评审

    doi：

    authors： Heusch G,Schulz R

    更新日期：1998-09-01 00:00:00

  • The effect of repeated tryptophan administration on body weight, food intake, brain lipid peroxidation and serotonin immunoreactivity in mice.

    abstract：:Tryptophan as a circulating precursor of serotonin (5-HT) may suppress food intake and body weight. Tryptophan administration can enhance the generation of reactive oxygen species (ROS) by inducing oxidative pathway in vivo and in vitro. We have examined the effect of repeated tryptophan administration on food consump...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-005-9103-5

    authors： Coşkun S,Ozer C,Gönül B,Take G,Erdoğan D

    更新日期：2006-06-01 00:00:00

  • Up-regulation of expression of translation factors--a novel molecular mechanism for cadmium carcinogenesis.

    abstract：:The molecular mechanisms potentially responsible for cadmium carcinogenesis were investigated by differential gene expression analysis of Balb/c-3T3 cells morphologically transformed with cadmium chloride. Differential display analysis of gene expression revealed overexpression of mouse Translation Initiation Factor 3...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1023/b:mcbi.0000007265.38475.f7

    authors： Joseph P,Lei YX,Ong TM

    更新日期：2004-01-01 00:00:00

  • Concerning the decreased D-3-hydroxybutyrate dehydrogenase activity in the liver and heart of hyperthyroid rats.

    abstract：:Whereas in rat liver mitochondria the hyperthyroid state causes an increase both in fatty acid unsaturation and in the Ea of D-3-hydroxybutyrate dehydrogenase and a decrease in phase transition temperature, in hyperthyroid rat heart mitochondria these changes are negligible. D-3-hydroxybutyrate dehydrogenase in both t...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/BF00226186

    authors： Lippolis R,Morini P,Conserva AR,Casalino E,Landriscina C

    更新日期：1990-03-27 00:00:00

  • Mitochondrial bioenergetics, uncoupling protein-2 activity, and reactive oxygen species production in the small intestine of a TNBS-induced colitis rat model.

    abstract：:Inflammatory bowel disease (IBD) is often associated with a decrease in energy-dependent nutrient uptake across the jejunum that serves as the main site for absorption in the small intestine. This association has prompted us to investigate the bioenergetics underlying the alterations in jejunal absorption in 2,4,6-tri...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-020-03749-z

    authors： Al Ojaimi Y,Khachab M,Bazzi S,Bahr GM,Echtay KS

    更新日期：2020-07-01 00:00:00

  • Identification of mitochondrial deficiency using principal component analysis.

    abstract：:The mitochondrial pathologies are a heterogeneous group of metabolic disorders that are characterized by anomalies of oxidative phosphorylation, especially in the respiratory chain. The diagnosis of these pathologies involves many investigations among which biochemical study is at present the main tool. However, the a...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：

    authors： Durrieu G,Letellier T,Antoch J,Deshouillers JM,Malgat M,Mazat JP

    更新日期：1997-09-01 00:00:00

  • The significance of aryl acylamidase activity of acetylcholinesterase in osteoblast differentiation and mineralization.

    abstract：:Osteoblast differentiation is an essential event in the developmental process, which is favoured by the production of extra cellular matrix proteins and various enzymes including discrete ones like acetylcholinesterase (AChE). Despite the fact that AChE facilitates osteoblast differentiation, the significance of its c...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-017-3167-x

    authors： Chinnadurai RK,Saravanaraman P,Boopathy R

    更新日期：2018-03-01 00:00:00

  • Osteopontin and cardiovascular system.

    abstract：:A matricellular protein, osteopontin (OPN), is expressed in response to mechanical stress and similar stimuli in the heart, integrates the inter-ECM signal transduction network of component cells, and maintains efficient contractility through quantitative and qualitative control of extracellular matrix (ECM) proteins....

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章,评审

    doi：10.1007/s11010-006-9368-3

    authors： Okamoto H

    更新日期：2007-06-01 00:00:00

  • Ghrelin and obestatin expression in oral squamous cell carcinoma: an immunohistochemical and biochemical study.

    abstract：:The underlying molecular mechanism of carcinogenesis in oral squamous cell carcinoma (OSCC) is poorly understood and appears to be controlled on many genetic, environmental, and hormonal factors. Obestatin and ghrelin, two recently discovered hormones, are co-expressed in endocrine cells. The purpose of this investiga...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-009-0381-1

    authors： Alnema MM,Aydin S,Ozkan Y,Dagli AF,Ozercan HI,Yildirim N,Sahin I,Karaoglu A,Kilic N,Yilmaz M,Ozercan MR,Donder E

    更新日期：2010-06-01 00:00:00

  • In vivo effects of vanadium in diabetic rats are independent of changes in PI-3 kinase activity in skeletal muscle.

    abstract：:The PI-3 kinase signalling pathway is an important pathway in mediating the glucoregulatory effects of insulin and skeletal muscle (SKM) is the major tissue involved in glucose utilization. In diabetes this pathway is impaired, either due to lack of insulin as in Type I diabetes, or due to insulin resistance as in Typ...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1023/a:1017913130401

    authors： Mohammad A,Bhanot S,McNeill JH

    更新日期：2001-07-01 00:00:00

  • Thyroid hormones stimulate Na+-Pi transport activity in rat renal brush-border membranes: role of membrane lipid composition and fluidity.

    abstract：:Antecedent studies have suggested that lipid composition and fluidity of cellular membranes of various organs are altered in response to thyroid hormone status. To date, the effects of thyroid hormone status on these parameters have not been examined in rat renal apical membrane in regard to sodium-dependent phosphate...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-005-3545-7

    authors： Prasad R,Kumar V

    更新日期：2005-01-01 00:00:00

  • Oxidative stress and TNF-alpha induce histone acetylation and NF-kappaB/AP-1 activation in alveolar epithelial cells: potential mechanism in gene transcription in lung inflammation.

    abstract：:Oxidants and inflammatory mediators such as tumour necrosis factor-alpha (TNF-alpha) activate nuclear factor kappa B (NF-kappaB) and activator protein-1 (AP-1) transcription factors, and enhance the expression of both pro-inflammatory and protective antioxidant genes. Remodelling of chromatin within the nucleus, contr...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：

    authors： Rahman I,Gilmour PS,Jimenez LA,MacNee W

    更新日期：2002-05-01 00:00:00

  • Effect of phase delay lighting rotation schedule on daily expression of per2, bmal1, rev-erbα, pparα, and pdk4 genes in the heart and liver of Wistar rats.

    abstract：:Under synchronized conditions daily rhythms run in precise phase relationships. Long lasting shift-work disturbs circadian rhythms and causes metabolism dysfunction. As a result of frequent shifts of the light (L):dark (D) cycle the circadian system has to adjust to a new regimen repeatedly, and organism can never ach...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-010-0636-x

    authors： Szántóová K,Zeman M,Veselá A,Herichová I

    更新日期：2011-02-01 00:00:00

  • Induction of AP-1 activity associated with c-Jun and JunB is required for mitogenesis induced by insulin and vanadate in SV40-transformed 3T3T cells.

    abstract：:Insulin and vanadate function as complete mitogens for SV40-transformed murine 3T3T (CSV3-1) cells but not for nontransformed 3T3T cells. Mitogenesis induced by insulin and vanadate in CSV3-1 cells is associated with the induction of the expression of protooncogenes c-jun and junB, two major AP-1 transcription factor ...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1023/a:1006889623326

    authors： Wang H,Xie Z,Scott RE

    更新日期：1997-03-01 00:00:00

  • PPARγ antagonist GW9662 induces functional estrogen receptor in mouse mammary organ culture: potential translational significance.

    abstract：:The nuclear receptor peroxisome proliferator-activated receptor gamma (PPARγ) plays a central role in regulating metabolism, including interaction with the estrogen receptor-α (ERα). Significantly, PPARγ activity can be modulated by small molecules to control cancer both in vitro and in vivo (Yin et al., Cancer Res 69...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-012-1466-9

    authors： Mehta RG,Peng X,Roy S,Hawthorne M,Kalra A,Alimirah F,Mehta RR,Kopelovich L

    更新日期：2013-01-01 00:00:00

  • Molecular changes to the rat renal cotransporters PEPT1 and PEPT2 due to ageing.

    abstract：:Renal PEPT1 and PEPT2 cotransporters play an important role in the balance of circulating body oligopeptides and selected peptidomimetic drugs. We aim to comprehensively characterise age-related changes of the renal PEPT cotransporters at the gene, protein, and functional level. Brush border membrane vesicles (BBMV) a...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-018-3413-x

    authors： Alghamdi OA,King N,Andronicos NM,Jones GL,Chami B,Witting PK,Moens PDJ

    更新日期：2019-02-01 00:00:00

  • Involvement of nuclear factor-1 (NF1) binding motif in the regucalcin gene expression of rat kidney cortex: the expression is suppressed by cisplatin administration.

    abstract：:The binding of nuclear factor on the promoter region of the regucalcin gene and the expression of regucalcin in the kidney cortex of rats was investigated. Nuclear extracts from kidney cortex were used for oligonucleotide competition gel mobility shift assay. An oligonucleotide between position -523 and -506 in the 5'...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1023/a:1011040805597

    authors： Misawa H,Yamaguchi M

    更新日期：2001-03-01 00:00:00

  • Purinergic system as a potential target for inflammation and toxicity induced by thymol in immune cells and tissues.

    abstract：:Thymol is a phytochemical component present in many plants used as food additive in order to promote animal growth due to its several biological properties. However, possible side effects of thymol remain poorly known limited to few reports. In this sense, we evaluated the enzymes of the purinergic signaling such as, ...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-018-3416-7

    authors： Baldissera MD,Souza CF,De Matos AFIM,Baldisserotto B,Stefani LM,da Silva AS

    更新日期：2019-02-01 00:00:00

  • The role of actin depolymerizing factor in advanced glycation endproducts-induced impairment in mouse brain microvascular endothelial cells.

    abstract：:Endothelial dysfunction is one of the most important pathological events during the development of several diabetic complications including stroke. The aim of this work was to investigate the role of actin depolymerizing factor (ADF) in advanced glycation endproducts (AGEs)-induced impairment in mouse brain microvascu...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-017-3019-8

    authors： Liu X,Zhang R,Di H,Zhao D,Wang J

    更新日期：2017-09-01 00:00:00

  • Ganglioside GM3 promotes HGF-stimulated motility of murine hepatoma cell through enhanced phosphorylation of cMet at specific tyrosine sites and PI3K/Akt-mediated migration signaling.

    abstract：:Ganglioside GM3 plays a well-documented and important role in the regulation of tumor cell proliferation, invasion, and metastasis by modulating tyrosine kinase growth factor receptors. However, the effect of GM3 on the hepatocyte growth factor receptor (HGFR, cMet) has not been fully delineated. In the current study,...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-013-1720-9

    authors： Li Y,Huang X,Zhong W,Zhang J,Ma K

    更新日期：2013-10-01 00:00:00

  • Equal reduction in infarct size by ethylisopropyl-amiloride pretreatment and ischemic preconditioning in the in situ rabbit heart.

    abstract：UNLABELLED:Inhibition of Na+/H+ exchange with amiloride analogues has been shown to provide functional protection during ischemia and reperfusion and to reduce infarct size in isolated rat hearts. In rat hearts, treatment with ethylisopropyl-amiloride (EIPA, a selective Na+/H- exchange inhibitor) was additive to the pr...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：

    authors： Munch-Ellingsen J,Løkebø JE,Bugge E,Ytrehus K

    更新日期：1998-09-01 00:00:00

  • Study on lipid peroxidation potential in different tissues induced by ascorbate-Fe2+: possible factors involved in their differential susceptibility.

    abstract：:Susceptibility of four major rat tissues to oxidative damage in terms of lipid peroxidation induced by in vitro by ascorbate-Fe2+ in homogenates and mitochondria has been examined. Lipid peroxidation, as assessed by thiobarbituric acid reactive substances (TBARS) and conjugated dienes was maximum in brain followed by ...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1023/a:1006895531504

    authors： Pushpendran CK,Subramanian M,Devasagayam TP,Singh BB

    更新日期：1998-01-01 00:00:00