Abstract：
:Pulmonary atresia (PA) with ventricular septal defect (VSD) is an extreme form of tetralogy of Fallot with characteristic right ventricular hypertrophy. To reduce the right ventricular overload, these children have to undergo staged corrective surgery to restore physiological pulmonary perfusion. We studied the degree of fibrosis by analysing the myocardial expression pattern (at mRNA and protein level) of the extracellular matrix proteins, collagen and fibronectin in biopsies taken at corrective surgery from 14 patients affected by PA,VSD. Expression analysis by RT-PCR showed significantly higher levels for collagen III (p = 0.03), whereas collagen Ialpha (p = 0.31) and fibronectin (p = 0.47) mRNA levels remained unaltered in PA, VSD patients as compared to age matched controls. Video image analysis of immunohistochemical staining showed unchanged interstitial levels for total collagen (p = 0.17) as well as for fibronectin (p = 0.13) in the patients with PA, VSD. However, peri-vascular staining for collagen (p < 0.01) and fibronectin (p = 0.02) represented as the peri-vascular stained area corrected for the vessel lumen area showed significantly decreased levels in the PA, VSD group as compared to controls. Our results indicate that the patients with PA, VSD have inadequate extracellular matrix support for their coronary blood vessels and perhaps due to an altered biosynthesis of collagen and fibronectin network.
journal_name
Mol Cell Biochemjournal_title
Molecular and cellular biochemistryauthors
Peters TH,de Jong PL,Klompe L,Berger RM,Saxena PR,Sharma HS,Bogers AJkeywords:
subject
Has Abstractpub_date
2003-09-01 00:00:00pages
27-32issue
1-2eissn
0300-8177issn
1573-4919journal_volume
251pub_type
杂志文章abstract：:Vaccinia virus (VV) and other pathogenic poxviruses encode for a complement control protein. The VV complement control protein or VCP, was one of the first soluble microbial proteins postulated to have an active role in the immunomodulation of the host defense. Since then, 2 other poxviruses, including variola virus a...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1023/a:1006844624825
更新日期：1998-08-01 00:00:00
abstract：:Norepinephrine (NE) is involved in many cardiovascular diseases such as congestive heart failure. We have recently reported that NE had a comitogenic effect in isolated cardiac fibroblasts, and that it activated p42/p44 mitogen activated protein kinase (MAPK). This study was designed to characterize a possible mechani...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1023/a:1021655023870
更新日期：2003-01-01 00:00:00
abstract：:Hypertension and related oxidative stress are involved in the pathogenesis of any renal diseases. Angiotensin-converting enzyme inhibitors have multi-directional renoprotective effects. In this study, we aimed to investigate whether lisinopril treatment has any biochemical alterations on renal tissue in L-NAME (Nε-nit...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-011-0760-2
更新日期：2011-06-01 00:00:00
abstract：:In an attempt to understand the functional role of individual transcription factors, we have carried out S1 nuclease analyses of in vitro synthesized RNA using partially purified HeLa cell factors and truncated 5S and tDNA. The results indicate that faithful initiation of 5S gene transcription depends on the presence ...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/BF00925972
更新日期：1993-04-21 00:00:00
abstract：:Purified mitochondria from rat liver were found to contain protein synthesis inhibitors, that could be extracted by disruption of mitochondrial membranes and fractionated by gel filtration into two fractions of low and high molecular weight. Small size inhibitors were also released from the latter peak by high ionic s...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/BF00223302
更新日期：1984-06-01 00:00:00
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journal_title：Molecular and cellular biochemistry
pub_type：
doi：10.1007/s11010-005-8210-7
更新日期：2005-11-01 00:00:00
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1023/a:1007081703928
更新日期：2000-05-01 00:00:00
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-006-9196-5
更新日期：2006-10-01 00:00:00
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-017-2954-8
更新日期：2017-06-01 00:00:00
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-006-9263-y
更新日期：2007-01-01 00:00:00
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-014-2118-z
更新日期：2014-10-01 00:00:00
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-012-1466-9
更新日期：2013-01-01 00:00:00
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/BF01741714
更新日期：1976-09-30 00:00:00
abstract：:Stress genes can be ascribed to have been generated by the organism for their intrinsic urge to survive against the changing environmental odds, during the evolutionary process. This concept has been supported by a large number of reports describing individual types of phenomena. These have been reconciled and globali...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章,评审
doi：
更新日期：1999-06-01 00:00:00
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-014-2117-0
更新日期：2014-10-01 00:00:00
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/BF00230325
更新日期：1992-10-07 00:00:00
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/BF00227902
更新日期：1996-04-12 00:00:00
abstract：:Pre-purified preparations of myoinositol-1-phosphate synthase (E.C. 5.5.1.4) from rat testes can be purified to homogeneity by first crystallizing the enzyme according to JAKOBY and then recrystallizing it at a pH value close to the isoelectric point while slowly increasing the temperature from 0 to 15 degrees C. This...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/BF00223357
更新日期：1979-12-14 00:00:00
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-017-3261-0
更新日期：2018-08-01 00:00:00
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-005-3459-4
更新日期：2005-01-01 00:00:00
abstract：:Activation of either the A(1) adenosine receptor (A(1)R) or the A(3) adenosine receptor (A(3)R), by their specific agonists CCPA and Cl-IB-MECA, respectively, protects cardiac cells in culture against ischemic injury. Yet the full protective mechanism remains unclear. In this study, we therefore examined the involveme...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-010-0568-5
更新日期：2010-12-01 00:00:00
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-006-9270-z
更新日期：2007-01-01 00:00:00
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-016-2891-y
更新日期：2017-03-01 00:00:00
abstract：:Changes in fatty acid composition of myocardial lipids were examined in rats with heart failure following myocardial infarction. Left ventricular systolic pressure (LVSP) was decreased and left ventricular end-diastolic pressure (LVEDP) was elevated 24 h, 1 and 12 weeks after left coronary artery ligation (CAL), sugge...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：
更新日期：1997-11-01 00:00:00
abstract：:To explore the effect of LYRM1 over-expression on basal and insulin-stimulated glucose uptake in rat skeletal muscle cells, and to understand the underlying mechanisms, Rat myoblasts (L6) transfected with either an empty expression vector (pcDNA3.1Myc/His B) or a LYRM1 expression vector were differentiated into myotub...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-010-0649-5
更新日期：2011-02-01 00:00:00
abstract：:Hydrogen sulfide (H2S) has been investigated widely in recent years. H2S plays a variety of roles in different biological systems, including cardiovascular system. It is the final product of amino acids metabolism, which contains sulfur-cysteine and homocysteine (Hcy). In human plasma, there are several various forms ...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-015-2364-8
更新日期：2015-06-01 00:00:00
abstract：:Ovarian cancer is one of the greatest causes of cancer death in women. The association of TMEM49 and ovarian cancer is poorly defined. Here, we reported that TMEM49 was significantly increased in ovarian tumor tissues compared to ovarian normal tissues. Furthermore, down-regulation of TMEM49 through RNA interference i...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-016-2684-3
更新日期：2016-05-01 00:00:00
abstract：:Five isoforms of CK2 may exist simultaneously in yeast cells: free catalytic subunits CK2α', CK2α and three holoenzymatic structures composed of αα'ββ', α(2)ββ' and α'(2)ββ'. Each isolated and purified form exhibits properties typical for CK2, but they differ in substrate specificity as well as in sensitivity to speci...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-011-0951-x
更新日期：2011-10-01 00:00:00
abstract：:Murine erythroleukemia cell lines derived from Friend virus infected mice can be induced to differentiate in vitro by numerous agents. Among these compounds are certain naturally occurring purines such as hypoxanthine or 1-methylhypoxanthine. We have extended these studies to other modified oxypurines and have identif...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/BF00220717
更新日期：1990-01-18 00:00:00
abstract：:Triplex forming oligonucleotides (TFO) are known to be potential agents for modifying gene function. In most instances they are utilized for repression of transcription. However hybrid molecules containing cis-acting elements in a duplex DNA in a hairpin form contiguously with the TFO can bind transcription factors in...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-005-7283-7
更新日期：2005-10-01 00:00:00
