当前位置： SCI文献检索 > MOLECULAR AND CELLULAR BIOCHEMISTRY期刊下所有文献 > Picroliv -- a natural product protects cells and regulates the gene expression during hypoxia/reoxygenation.

Picroliv -- a natural product protects cells and regulates the gene expression during hypoxia/reoxygenation.

Abstract：

:Cellular adaptation to hypoxia involves regulation of specific genes such as vascular endothelial growth factor (VEGF), erythropoietin (EPO) and hypoxia inducible factor (HIF)-1 . In this study, we have evaluated the protective effect of picroliv (a purified iridoid glycoside fraction from roots of Picrorhiza kurrooa with hepatoprotective, anti-inflammatory and antioxidant properties) against hypoxic injury by examining lactate dehydrogenase (LDH) release in Hep 3B and Glioma cells. The expression of hypoxia regulated genes, VEGF and HIF-1 was studied in human umbilical vein endothelial cells (HUVEC), Hep 3B and Glioma cells. Picroliv reduced the cellular damage caused by hypoxia as revealed by a significant reduction in LDH release compared to untreated control. The expression of VEGF and HIF-1 subunits (HIF-1alpha and HIF-1beta) was enhanced by treatment with picroliv during normoxia and hypoxia in HUVEC and Hep 3B cells and on reoxygenation the expression of these genes was significantly reduced as revealed by mRNA analysis using RT-PCR. Simultaneous treatment with picroliv during hypoxia inhibited VEGF and HIF-1 expression in Glioma cells whereas the expression was not reduced by picroliv treatment during reoxygenation as evidenced by both RT-PCR and Northern hybridization. VEGF expression as revealed by immunofluorescence studies correlates well with the regulations observed in the mRNA expression. We have also examined the kinase activity of tyrosine phosphorylated proteins and protein kinase C (PKC) in Glioma cells treated with picroliv during hypoxia/reoxygenation. A selective inhibition of protein tyrosine kinase activity leading to tyrosine dephosphorylation of several proteins including 80 kd protein, and a reduction in PKC was seen in cells treated with picroliv and hypoxia. These findings suggest that picroliv may act as a protective agent against hypoxia/reoxygenation induced injuries, and the underlying mechanism may involve a novel signal transduction pathway.

journal_name

Mol Cell Biochem

journal_title

Molecular and cellular biochemistry

authors

Gaddipati JP,Madhavan S,Sidhu GS,Singh AK,Seth P,Maheshwari RK

doi

10.1023/a:1006982028460

keywords:

subject

Has Abstract

pub_date

1999-04-01 00:00:00

pages

271-81

issue

1-2

eissn

0300-8177

issn

1573-4919

journal_volume

194

pub_type

杂志文章

相关文献

MOLECULAR AND CELLULAR BIOCHEMISTRY文献大全
  • Role of reactive oxygen species in cardiovascular aging.

    abstract：:Biochemical and structural changes occurring in the myocardium with aging are mainly resulting from the association of a general tissue atrophy with the hypertrophy of the remaining myocytes. Whilst hypertrophy seems to be a compensatory process to the loss of cardiomyocytes and to a mild systolic hypertensive conditi...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章,评审

    doi：10.1007/BF00240046

    authors： Muscari C,Giaccari A,Giordano E,Clô C,Guarnieri C,Caldarera CM

    更新日期：1996-07-01 00:00:00

  • Effects of Mg2+ and adenine nucleotides on thymidylate synthetase from different mouse tumors.

    abstract：:Magnesium ions variably influenced activity of highly purified thymidylate synthetase preparations from different mouse tumors, activating the enzyme from Ehrlich ascites carcinoma (EAC) cells and inhibiting the enzyme from L1210 and L5178Y cells and from 5-fluorodeoxyuridine (FdUrd)-resistant EAC cells. In the presen...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/BF00226300

    authors： Rode W,Jastreboff MM

    更新日期：1984-01-01 00:00:00

  • A cell-based reporter assay for the identification of protein kinase C activators and inhibitors.

    abstract：:Transmission of extra cellular signals across biological membranes results in the generation of lipid metabolites which in turn influence specific cellular events such as cell growth or differentiation. Many of these lipid messengers can activate protein kinase C (PKC) isozymes of which one function is to perpetuate t...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/BF00926176

    authors： Sista P,Edmiston S,Darges JW,Robinson S,Burns DJ

    更新日期：1994-12-21 00:00:00

  • Inhibition of mitophagy decreases survival of Caenorhabditis elegans by increasing protein aggregation.

    abstract：:Autophagy of mitochondria, i.e., mitophagy, plays a crucial role in coping with stressors in the aging process, metabolic disturbances, and neurological disorders. Impairments of the process might consequently lead to enhanced accumulation of aged and aggregated proteins and reduced cellular integrity in response to s...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-018-3418-5

    authors： Civelek M,Mehrkens JF,Carstens NM,Fitzenberger E,Wenzel U

    更新日期：2019-02-01 00:00:00

  • Potential of C1QTNF1-AS1 regulation in human hepatocellular carcinoma.

    abstract：:The aim of our study is to explore the regulation of C1QTNF1-AS1 on its target miR-221-3p/SOCS3 in human hepatocellular carcinoma (HCC). To explore the underlying molecular regulation of non-coding RNA for HCC, differentially expressed patterns of lncRNAs and genes were examined by RNA-seq. GO and KEGG pathway analysi...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-019-03569-w

    authors： Han W,Yu G,Meng X,Hong H,Zheng L,Wu X,Zhang D,Yan B,Ma Y,Li X,Wang Q

    更新日期：2019-10-01 00:00:00

  • The siRNA-mediated downregulation of N-Ras sensitizes human melanoma cells to apoptosis induced by selective BRAF inhibitors.

    abstract：:The clinical benefit of selective BRAF inhibitor therapies is limited by the emergence of drug resistance. Here, we investigated the molecular basis underlying the acquired resistance to a BRAF inhibitor by comparing the signaling pathways in the parental A375P cells and the resistant subline (A375P/Mdr). We demonstra...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-014-2034-2

    authors： Ahn JH,Lee M

    更新日期：2014-07-01 00:00:00

  • Chemistry and biology of E. coli ribosomal protein L12.

    abstract：:E. coli ribosomal protein L12, because of its unique features, has been studied in more detail than perhaps any of the other ribosomal proteins. Unlike the other ribosomal proteins that are generally present in stoichiometric amounts, there are four copies of L12 per ribosome, some of which are acetylated on the N-ter...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章,评审

    doi：10.1007/BF02354831

    authors： Brot N,Weissbach H

    更新日期：1981-04-13 00:00:00

  • Redox status in acute ischemic stroke: correlation with clinical outcome.

    abstract：:Connection between oxidative stress and clinical outcome in acute ischemic stroke (AIS) has been poorly investigated. This study was aimed to assess redox state (through measurement of oxidative stress markers) of patients with acute ischemic stroke during different stages of follow-up period, and to find association ...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-015-2425-z

    authors： Paspalj D,Nikic P,Savic M,Djuric D,Simanic I,Zivkovic V,Jeremic N,Srejovic I,Jakovljevic V

    更新日期：2015-08-01 00:00:00

  • Attenuation of age-related increase of protein carbonylation in the liver of mice by melatonin and curcumin.

    abstract：:Protein carbonyls are formed as a consequence of the oxidative modification of proteins by reactive oxygen species and are commonly used as a marker of protein oxidation in cells and tissues. Melatonin has free radical scavenging ability besides its classical role as a hormonal signaling agent. Curcumin, a phytochemic...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-013-1668-9

    authors： Dkhar P,Sharma R

    更新日期：2013-08-01 00:00:00

  • Anticancer effect of calycopterin via PI3K/Akt and MAPK signaling pathways, ROS-mediated pathway and mitochondrial dysfunction in hepatoblastoma cancer (HepG2) cells.

    abstract：:Calycopterin is a flavonoid compound isolated from Dracocephalum kotschyi that has multiple medical uses, as an antispasmodic, analgesic, anti-hyperlipidemic, and immunomodulatory agents. However, its biological activity and the mechanism of action are poorly investigated. Herein, we investigated the apoptotic effect ...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-014-2166-4

    authors： Esmaeili MA,Farimani MM,Kiaei M

    更新日期：2014-12-01 00:00:00

  • Lipid peroxidation of erythrocytes during anemia of the hamsters infected with Leishmania donovani.

    abstract：:Visceral leishmaniasis has been found to be associated with severe anemia and premature lysis of erythrocytes. Peroxidative damage of red cells has been noted in several hemolytic anemias. Present study shows enhanced formation of methemoglobin in hamsters infected with Leishmania donovani. Increased formation of malo...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/BF00944601

    authors： Biswas T,Pal JK,Naskar K,Ghosh DK,Ghosal J

    更新日期：1995-05-24 00:00:00

  • Uteroferrin contains complex and high mannose-type oligosaccharides when synthesized in vitro.

    abstract：:Mature uteroferrin (Uf; Mr = 35,500) is a progesterone-induced acid phosphatase secreted by the pig uterus. It contains a single, unphosphorylated, high mannose-type oligosaccharide. Endometrial explants cultured in vitro secrete Uf with a Mr of 37,000 (37k Uf) having phosphorylated high mannose oligosaccharides. In t...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/BF00227750

    authors： Baumbach GA,Saunders PT,Ketcham CM,Bazer FW,Roberts RM

    更新日期：1991-07-10 00:00:00

  • Microassay for malignant hyperthermia susceptibility: hypersensitive ligand-gating of the Ca channel in muscle sarcoplasmic reticulum causes increased amounts and rates of Ca-release.

    abstract：:A crude preparation of heavy sarcoplasmic reticulum (HSR) was isolated using 1 gram of muscle obtained from swine susceptible to malignant hyperthermia (MH) and from control swine. The caffeine and ATP concentration-dependency of Ca-release was determined using suction filtration with radioisotopic 45Ca as a tracer. R...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/BF00223492

    authors： O'Brien PJ

    更新日期：1990-03-05 00:00:00

  • The connexin43 gap junction protein is phosphorylated by protein kinase A and protein kinase C: in vivo and in vitro studies.

    abstract：:There is general agreement that the connexin43 gap junction protein is a substrate for phosphorylation by protein kinase C but there is no similar consensus regarding the action of protein kinase A. Our previous studies demonstrated that channels formed by connexin43 were reversibly gated in response to microinjected ...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1023/a:1019902920693

    authors： Shah MM,Martinez AM,Fletcher WH

    更新日期：2002-09-01 00:00:00

  • Detection of a sequence involved in actin-binding and phosphoinositide-binding in the N-terminal side of cofilin.

    abstract：:Cofilin is an actin-binding protein of low molecular weight which is widely distributed in eukaryotes and is deeply involved in the dynamics of actin assembly in the cytoplasm. The actin-binding ability of cofilin is inhibited by inositol phosphates (PIP2), and the PIP2- and actin-binding site(s) has been localized in...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：

    authors： Kusano K,Abe H,Obinata T

    更新日期：1999-01-01 00:00:00

  • miR-155 promotes the growth of osteosarcoma in a HBP1-dependent mechanism.

    abstract：:Osteosarcoma (OS) is a type of malignant tumor arising from soft-tissues of bone and displays poor prognosis in most cases. However, the molecular mechanism by which OS initiates and progresses is still not completely elucidated. miR-155 has been shown to be overexpressed in OS specimen and cell lines. Our study is in...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-015-2344-z

    authors： Sun X,Geng X,Zhang J,Zhao H,Liu Y

    更新日期：2015-05-01 00:00:00

  • Noradrenaline and its end metabolite 3-methoxy-4-hydroxyphenylglycol inhibit lymphocyte chemotaxis: role of alpha- and beta-adrenoreceptors.

    abstract：:The capacity of noradrenaline (NA) and its end metabolite 3-methoxy-4-hydroxyphenylglycol (MHPG) to modulate the chemotaxis of lymphocytes from a primary immunocompetent organ (thymus) and a secondary one (spleen) was investigated over a range of concentrations from 10(-12) M to 10(-5) M. Lymphocyte chemotaxis was eva...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1023/a:1027349904589

    authors： García JJ,del Carmen Sáez M,De la Fuente M,Ortega E

    更新日期：2003-12-01 00:00:00

  • Reconstitution of rat liver 60S ribosomal subunits following disassembly by dimethylmaleic anhydride.

    abstract：:Modification of 60S ribosomal subunits from rat liver with dimethylmaleic anhydride (60 mumols/ml) is accompanied by release of 35% of the protein. The acidic ribosomal proteins, as well as 9 basic proteins, are selectively liberated from the ribosomal subunits. Reconstitution of the protein-deficient particles with t...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/BF00218133

    authors： Garrido S,González PJ,Palacián E,Hernández F

    更新日期：1990-02-09 00:00:00

  • The properties of mnemiopsin, a bioluminescent and light sensitive protein purified by hollow fiber techniques.

    abstract：:A calcium activated photoprotein, termed mnemiopsin, which emits bioluminescence upon the addition of calcium ion, has been isolated from the Ctenophore, Memiopsis leidyi, and purified by hollow fiber techniques. The system is similar to aequorin, from the jellyfish Aequorea, except that mnemiopsin can be light-inacti...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/BF00232600

    authors： Girsch SJ,Hastings JW

    更新日期：1978-04-11 00:00:00

  • Soluble metals as well as the insoluble particle fraction are involved in cellular DNA damage induced by particulate matter.

    abstract：:Exposure to ambient particulate matter has been reported to be associated with increased rates of lung cancer. Previously we showed that total suspended particulate matter (PM) induces oxidative DNA damage in epithelial lung cells. The aim of the present study was to further investigate the mechanism of PM-induced DNA...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：

    authors： Knaapen AM,Shi T,Borm PJ,Schins RP

    更新日期：2002-05-01 00:00:00

  • Semiquinone derivative isolated from Bacillus sp. INM-1 protects cellular antioxidant enzymes from γ-radiation-induced renal toxicity.

    abstract：:This study was focused to evaluate protection of indigenous antioxidant system of mice against gamma radiation-induced oxidative stress using a semiquinone (SQGD)-rich fraction isolated from Bacillus sp. INM-1. Male C57bl/6 mice were administered SQGD (50 mg/kgb.w.i.p.) 2 h before irradiation (10 Gy) and modulation in...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-013-1622-x

    authors： Mishra S,Reddy DS,Jamwal VS,Bansal DD,Patel DD,Malhotra P,Gupta AK,Singh PK,Jawed S,Kumar R

    更新日期：2013-07-01 00:00:00

  • Studies on the adrenal cortex of hypophysectomized rats: a model for abnormal cellular atrophy and death.

    abstract：:Biochemical and ultrastructural studies indicate that the atrophy of adrenal cortex in hypoyhysectomized rats involves the following changes: (1) One to two days after hypophysectomy, there is loss of "template activity" resulting from cumulative DNA-damage and heterochromatinization. In vivo ACTH-administration led t...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/BF01793330

    authors： Tchen TT,Chan SW,Kuo TH,Mostafapour KM,Drzewiecki VH

    更新日期：1977-04-12 00:00:00

  • Up-regulation of expression of translation factors--a novel molecular mechanism for cadmium carcinogenesis.

    abstract：:The molecular mechanisms potentially responsible for cadmium carcinogenesis were investigated by differential gene expression analysis of Balb/c-3T3 cells morphologically transformed with cadmium chloride. Differential display analysis of gene expression revealed overexpression of mouse Translation Initiation Factor 3...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1023/b:mcbi.0000007265.38475.f7

    authors： Joseph P,Lei YX,Ong TM

    更新日期：2004-01-01 00:00:00

  • Characteristics of an ADP receptor mediating platelet activation.

    abstract：:Adenosine diphosphate (ADP) is known to induce platelet shape change, aggregation and fibrinogen binding, followed by secretion. These processes are mediated by the binding of ADP to an externally oriented protein of the platelet plasma membrane. An affinity analog of ATP, a competitive inhibitor of the action of ADP,...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章,评审

    doi：10.1007/BF00231307

    authors： Colman RW,Figures WR

    更新日期：1984-01-01 00:00:00

  • Splicing factors differentially expressed in psoriasis alter mRNA maturation of disease-associated EDA+ fibronectin.

    abstract：:The EDA+ fibronectin splicing variant is overexpressed in psoriatic non-lesional epidermis and sensitizes keratinocytes to mitogenic signals. However, regulation of its abundance is only partially understood. In our recent cDNA microarray experiment, we identified three SR-rich splicing factors-splicing factor, argini...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-017-3090-1

    authors： Szlavicz E,Szabo K,Groma G,Bata-Csorgo Z,Pagani F,Kemeny L,Szell M

    更新日期：2017-12-01 00:00:00

  • Schisandrin B induced antioxidant response is partly mediated by cytochrome P-4502E1 catalyzed reaction in mouse liver.

    abstract：:In order to explore the role of cytochrome P-450 (CYP) 2E1 in schisandrin B (Sch B)-induced antioxidant and heat shock responses, the effects of Sch B treatment on hepatic mitochondrial glutathione antioxidant status (mtGAS) and heat shock protein (Hsp)25/70 expression were compared between wild-type and cyp2e1 knock-...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-006-2957-3

    authors： Chiu PY,Leung HY,Poon MK,Lee SS,Ko KM

    更新日期：2006-12-01 00:00:00

  • Reduced human lymphocyte blastogenesis and enhancement of adenosine triphosphate (ATP) by L-carnitine.

    abstract：:Carnitine is associated with lipid synthesis and its deficiency may lead to cardiomegaly with parenchymal lipid in the heart, kidney and liver. In our study we found that pretreatment of peripheral blood mononuclear cells (PBMC) with serial dilutions of L-Carnitine (100 micrograms/ml-1 pg/ml) inhibits, in a dose-depen...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/BF01075718

    authors： Conti P,Reale M,Stuard S,Spoto G,Picerno F,Ferrara T,Placido FC,Barbacane RC,Albertazzi A,Errichi BM

    更新日期：1994-02-09 00:00:00

  • Protein kinases: mechanisms and downstream targets in inflammation-mediated obesity and insulin resistance.

    abstract：:Obesity-induced low-grade inflammation (metaflammation) impairs insulin receptor signaling. This has been implicated in the development of insulin resistance. Insulin signaling in the target tissues is mediated by stress kinases such as p38 mitogen-activated protein kinase, c-Jun NH2-terminal kinase, inhibitor of NF-k...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章,评审

    doi：10.1007/s11010-016-2878-8

    authors： Nandipati KC,Subramanian S,Agrawal DK

    更新日期：2017-02-01 00:00:00

  • Inhibitory effect of Styrax Japonica Siebold et al. Zuccarini glycoprotein (38 kDa) on interleukin-1β and induction proteins in chromium(VI)-treated BNL CL.2 cells.

    abstract：:Chromium(VI) [Cr(VI)] induces chronic inflammation in hepatocytes. Inflammation has been shown to play an important role in tumorigenesis, tumor progression, and metastasis. To examine the effects of the Styrax Japonica Siebold et al. Zuccarini (SJSZ) glycoprotein on inflammation in BNL CL.2 cells, we evaluated the ac...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-012-1324-9

    authors： Lee J,Lim KT

    更新日期：2012-08-01 00:00:00

  • The cell cycle regulator protein P16 and the cellular senescence of dental follicle cells.

    abstract：:Cellular senescence is a restricting factor for regenerative therapies with somatic stem cells. We showed previously that the onset of cellular senescence inhibits the osteogenic differentiation in stem cells of the dental follicle (DFCs), although the mechanism remains elusive. Two different pathways are involved in ...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-017-3134-6

    authors： Morsczeck C,Hullmann M,Reck A,Reichert TE

    更新日期：2018-02-01 00:00:00