Abstract：
:Osteosarcoma (OS) is a type of malignant tumor arising from soft-tissues of bone and displays poor prognosis in most cases. However, the molecular mechanism by which OS initiates and progresses is still not completely elucidated. miR-155 has been shown to be overexpressed in OS specimen and cell lines. Our study is intended to explore the role of miR-155 in OS etiology. The data confirmed that miR-155 abundance is higher in OS samples than non-cancerous bone tissue. Inhibition of miR-155 suppressed the proliferation of OS cells and cell cycle progression in vitro, and the growth of OS xenografts in vivo. Wnt pathway was suppressed in OS cells by miR-155 inhibitors. HMG-box transcription factor 1 (HBP1), a strong Wnt pathway suppressor, was found to be a target of miR-155. Restoration of HBP1 abolished the effect of miR-155 on OS cells. Finally, miR-155 levels in OS tissues and serum are both inversely associated with the survival of OS patients. Collectively, miR-155 was identified to be among the list of OS-related oncogenic miRNAs, and HBP1-mediated Wnt signaling is involved with the role of miR-155 in OS progression.
journal_name
Mol Cell Biochemjournal_title
Molecular and cellular biochemistryauthors
Sun X,Geng X,Zhang J,Zhao H,Liu Ydoi
10.1007/s11010-015-2344-zsubject
Has Abstractpub_date
2015-05-01 00:00:00pages
139-47issue
1-2eissn
0300-8177issn
1573-4919journal_volume
403pub_type
杂志文章abstract：:The pathophysiologic importance of insulin resistance in diseases such as obesity and diabetes mellitus has led to great interest in defining the mechanism of insulin action as well as the means to overcome the biochemical defects responsible for the resistance. Vanadium compounds have been discovered to mimic many of...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章,评审
doi：
更新日期：1998-05-01 00:00:00
abstract：:In previous publications from our laboratory, we reported that a soluble, cytochrome P-450-dependent fatty acid monooxygenase from Bacillus megaterium ATCC 14581 can be induced by phenobarbital and a variety of other barbiturates. The tested barbiturates showed an excellent correlation between increasing lipophilicity...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/BF00220988
更新日期：1985-05-01 00:00:00
abstract：:Transforming Growth Factor-beta (TGF-beta) plays an essential role in differentiation of dental pulp cells into odontoblasts during reparative dentine formation. However, the mechanism by which TGF-beta stimulates dental repair remains rather obscure. Human dental pulp cells were used as an in vitro model in the prese...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-006-9193-8
更新日期：2006-10-01 00:00:00
abstract：:Ehrlich ascites tumor cells incorporate [methyl-3H]thymidine into DNA independently of exogenous growth factors or fetal calf serum. Using an acid/ethanol extraction procedure we have obtained from these tumor cells a fraction that induces both the proliferation and the formation of cell foci by Swiss 3T3 mouse fibrob...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1023/a:1006809604193
更新日期：1997-05-01 00:00:00
abstract：:A wide variety of metals have been reported to act as mutagenic and carcinogenic agents in both human and animal studies. The underlying mechanisms are being extensively investigated. Recently, a new sub-discipline of molecular carcinogenesis has surfaced and new techniques and instruments are being developed which al...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章,评审
doi：10.1023/b:mcbi.0000007255.72746.a6
更新日期：2004-01-01 00:00:00
abstract：:It has been shown that elevation of plasma methionine (Met) and its metabolites may occur in several genetic abnormalities. In this study we investigated the in vitro and in vivo effects of the Met and methionine sulfoxide (MetO) on oxidative stress parameters in the liver of rats. For in vitro studies, liver homogena...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-013-1777-5
更新日期：2013-12-01 00:00:00
abstract：:Insulin receptor binding was examined in the microvillous membranes of mid-term (20-22 weeks of gestation, MT) and full-term (FT) placentas from patients with gestational diabetes mellitus (GDM) and in normal pregnant control (N). Mid-term placentas were obtained from patients who have had spontaneous abortion. The ma...
journal_title：Molecular and cellular biochemistry
pub_type： 临床试验,杂志文章
doi：10.1007/BF01076892
更新日期：1995-10-04 00:00:00
abstract：:Cytochrome c oxidase (COX), like other multi-subunit components of the respiratory chain, is controlled by both the nuclear and the mitochondrial genome. In order to find wether there is a close relationship between mRNAs encoded by the nucleus and by the mitochondrion, and between these mRNAs and enzyme activity, we ...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/BF02424572
更新日期：1991-09-18 00:00:00
abstract：:Recent studies suggest JAK2 signaling may be a therapeutic target for treatment of gastric cancer (GC). However, the exact roles of JAK2 in gastric carcinogenesis are not very clear. Here, we have targeted JAK2 to be silenced by shRNA and investigated the biological functions and related mechanisms of JAK2 in GC cell ...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-013-1881-6
更新日期：2014-02-01 00:00:00
abstract：:Lipids form an essential part of the biomembrane and it is of paramount importance to study their conformational aspects. It is found that the present methods of nomenclature for lipids are totally inadequate for describing these diverse amphipathic molecules. Further the existing methods are incompatible in terms of ...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章,评审
doi：10.1007/BF00228093
更新日期：1989-11-23 00:00:00
abstract：:Various factors appear to control muscle energetics, often in conjunction. This calls for a quantitative approach of the type provided by Metabolic Control Analysis for intermediary metabolism and mitochondrial oxidative phosphorylation. To the extent that direct transfer of high energy phosphates and spatial organiza...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/BF01816949
更新日期：1995-02-23 00:00:00
abstract：:Control of mitochondrial respiration depends on ADP availability to the F1-ATPase. An electrochemical gradient of ADP and ATP across the mitochondrial inner membrane is maintained by the adenine nucleotide translocase which provides ADP to the matrix for ATP synthesis and ATP for energy-dependent processes in the cyto...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章,评审
doi：10.1007/BF00219314
更新日期：1988-06-01 00:00:00
abstract：:There is a wealth of information regarding the import and export of nuclear proteins in general. Nevertheless, the available data that deals with the nucleocytoplasmic movement of steroid hormone receptors remains highly limited. Some research findings reported during the past five years have succeeded in identifying ...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章,评审
doi：10.1023/b:mcbi.0000026060.81570.35
更新日期：2004-05-01 00:00:00
abstract：:Drosophila melanogaster protein kinase CK2 (DmCK2) is a Ser/Thr protein kinase composed of catalytic alpha and regulatory beta subunits associated as an alpha2beta2 heterotetramer. Using the two hybrid system, we have screened a Drosophila embryo cDNA library in order to identify proteins that interact with DmCK2alpha...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：
更新日期：2001-11-01 00:00:00
abstract：:Krüppel-like factor 4 (KLF4), a zinc finger transcription factor, has been implicated in the inflammation mediated by macrophages and endothelial cells by regulating the expression of inflammatory mediators. Here, we investigated whether KLF4 affects the expression of inducible nitric oxide synthase (iNOS), an importa...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-017-3115-9
更新日期：2018-01-01 00:00:00
abstract：:The uptake of glucose and gluconate is under inductive control in Pseudomonas putida. Glucose, gluconate, and 2-ketogluconate were each good nutritional inducers of these transport abilities. Glucose and gluconate uptake obeyed saturation kinetics: the apparent Km for glucose was 6 mM and that for gluconate was 0.5 mM...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/BF01732164
更新日期：1975-04-30 00:00:00
abstract：:Hydrocortisone hemisuccinate within 4 hours after in vivo administration produced an increase in precursor incoporation into rat thymus RNA and proteins in the whole animal. From these results, together with information obtained from measurements of the tyrosine aminotransferase activity and the action of mitomycin C ...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/BF01751307
更新日期：1975-12-31 00:00:00
abstract：:In the eluted fractions of histone-treated crude extracts separated by Sephadex G-200 filtration, multiple protein kinase (PK) activities, including three from brain and two from skeletal muscle, were augmented by both S-100 protein and parvalbumin on the phosphorylation of endogenous substrates. One additional PK act...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/BF00219324
更新日期：1986-06-01 00:00:00
abstract：:Fenofibrate, a selective (1)PPAR-alpha activator, is prescribed to treat human dyslipidemia. The aim of this study was to delineate the mechanism of fenofibrate-mediated reductions in adiposity, improvements in insulin sensitivity, and lowering of triglycerides (TG) and free fatty acids (FFA) and to investigate if the...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-005-9053-y
更新日期：2006-04-01 00:00:00
abstract：:The exchange of protons and deuterons by phosphoglucoisomerase during the single passage conversion of D-[2-13C,1-2H]fructose 6-phosphate in H2O or D-[2-13C]fructose 6-phosphate in D2O to D-[2-13C]glucose 6-phosphate, as coupled with the further generation of 6-phospho-D-[2-13C]gluconate in the presence of excess gluc...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/BF00227479
更新日期：1991-05-15 00:00:00
abstract：:It has been recently hypothesized that in PIH a placental oxidant-antioxidant imbalance might cause the release of lipoperoxidation products into the circulation, with subsequent damage of endothelial cell membranes. In this hypothesis the endothelial cell and further increase in circulating lipoperoxide levels, which...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/BF00925951
更新日期：1994-02-23 00:00:00
abstract：:In order to explore the role of cytochrome P-450 (CYP) 2E1 in schisandrin B (Sch B)-induced antioxidant and heat shock responses, the effects of Sch B treatment on hepatic mitochondrial glutathione antioxidant status (mtGAS) and heat shock protein (Hsp)25/70 expression were compared between wild-type and cyp2e1 knock-...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-006-2957-3
更新日期：2006-12-01 00:00:00
abstract：:Several tyrosine phosphatases control cell motility; understanding their signaling helps to decipher cancer mechanisms. Previously, we found that the negative regulation of migration exerted by PTPN12 in ovarian cancer SKOV-3 cells involves direct FAK Y397 targeting, in HER2-dependent way. In this study, we describe t...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-012-1537-y
更新日期：2013-03-01 00:00:00
abstract：:Renilla luciferase reporter is a widely used internal control in dual luciferase reporter assay system, where its transcription is driven by a constitutively active promoter. However, the authenticity of the Renilla luciferase response in some experimental settings has recently been questioned. Testicular receptor 4 (...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-017-2961-9
更新日期：2017-06-01 00:00:00
abstract：:Lithostathine may play a physiological role in preventing the precipitation of excess calcium in the pancreatic juice. The hypothesis has been advanced that in chronic calcifying pancreatitis the abnormal biosynthesis of lithostathine might be the original defect to which genetic proneness to the disease may be ascrib...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1023/a:1006843309128
更新日期：1998-08-01 00:00:00
abstract：:Four situations in which membrane transport is altered by disease are discussed: (a) non-specific leaks induced by poreforming agents; (b) glucose transport and cellular stress; (c) Ca2+-ATPase and hypertension; (d) Na+ channels and HSV infection. ...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章,评审
doi：10.1007/BF00228073
更新日期：1989-11-23 00:00:00
abstract：:We report here a study of the incorporation and metabolism of various long chain fatty acids in SK-Hep-1 cultured hepatoma cells. Medium supplementation with radiolabelled palmitic, stearic, linoleic, alpha-linolenic and eicosa-8,11,14-trienoic acids (1 microM, 24 H) resulted in an active uptake of each of these precu...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/BF00230749
更新日期：1992-11-18 00:00:00
abstract：:Resveratrol (trans-3,5,4'-trihydroxystilbene), a compound found largely in the skins of red grapes and wines, possesses anti-cancer and anti-angiogenic properties and protects the cardiovascular system. However, the molecular mechanisms by which resveratrol inhibits angiogenesis are currently subjects of intense inves...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-009-0300-5
更新日期：2010-04-01 00:00:00
abstract：:It has been recently shown that long-term thyroxine administration increases the tolerance of the heart to ischaemia. The present study investigated whether thyroxine induced cardioprotection involves alterations in the pattern of p38 mitogen activated protein kinase (p38MAPK) and c-Jun NH2-terminal kinases (JNKs) act...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：
更新日期：2003-01-01 00:00:00
abstract：:We report the isolation of a full length cDNA from cardiac muscle that encodes a approximately 73 kDa calcium/calmodulin (CaM) dependent kinase IIbeta isoform (CaMKIIbeta(C)) that was generated by alternative splicing of the CaMKIIbeta gene. Antipeptide antibodies raised to specific regions of the kinase identified a ...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-005-5234-y
更新日期：2005-02-01 00:00:00
