Abstract:
:We report the isolation of a full length cDNA from cardiac muscle that encodes a approximately 73 kDa calcium/calmodulin (CaM) dependent kinase IIbeta isoform (CaMKIIbeta(C)) that was generated by alternative splicing of the CaMKIIbeta gene. Antipeptide antibodies raised to specific regions of the kinase identified a 73 kDa kinase polypeptide in cardiac SR. Anti-alpha kinase anchoring protein (alphaKAP) antibodies identified a 25 kDa polypeptide in cardiac SR and RT-PCR followed by sequence analysis confirmed the presence of a full length alphaKAP encoding transcript in myocardium. Protein interaction assays revealed that the 73 kDa CaMKIIbeta(C) binds GAPDH to modulate the production of NADH in a Ca2+/CaM dependent reaction. The presence of a CaMKIIbeta isoform that can target the SR presumably via its membrane anchor alphaKAP defines a previously unrecognized Ca2+/CaM regulatory system in myocardium.
journal_name
Mol Cell Biochemjournal_title
Molecular and cellular biochemistryauthors
Singh P,Leddy JJ,Chatzis GJ,Salih M,Tuana BSdoi
10.1007/s11010-005-5234-ykeywords:
subject
Has Abstractpub_date
2005-02-01 00:00:00pages
215-21issue
1-2eissn
0300-8177issn
1573-4919journal_volume
270pub_type
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