Abstract:
:Modification of 60S ribosomal subunits from rat liver with dimethylmaleic anhydride (60 mumols/ml) is accompanied by release of 35% of the protein. The acidic ribosomal proteins, as well as 9 basic proteins, are selectively liberated from the ribosomal subunits. Reconstitution of the protein-deficient particles with the corresponding split proteins is accompanied by substantial recovery of the original polyphenylalanine synthetic activity. The described reconstitution procedure can be used to investigate the roles played by the released proteins and the functional similarities of proteins from different sources. Hybrid reconstitution of residual ribosomal particles from rat liver or yeast with the corresponding heterologous split proteins produces subunits which have incorporated heterologous proteins but are inactive in polyphenylalanine synthesis.
journal_name
Mol Cell Biochemjournal_title
Molecular and cellular biochemistryauthors
Garrido S,González PJ,Palacián E,Hernández Fdoi
10.1007/BF00218133subject
Has Abstractpub_date
1990-02-09 00:00:00pages
159-67issue
2eissn
0300-8177issn
1573-4919journal_volume
92pub_type
杂志文章abstract::Insulin and vanadate function as complete mitogens for SV40-transformed murine 3T3T (CSV3-1) cells but not for nontransformed 3T3T cells. Mitogenesis induced by insulin and vanadate in CSV3-1 cells is associated with the induction of the expression of protooncogenes c-jun and junB, two major AP-1 transcription factor ...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1023/a:1006889623326
更新日期:1997-03-01 00:00:00
abstract::In the infarcted rat heart, the increase of NO occurs in the hypertrophied myocardium of non-infarcted areas and its antihypertrophic efficacy has been well established. As another endogenous regulator and the reliable index of heart pathology, B-type natriuretic peptide also exhibits the antihypertrophic properties i...
journal_title:Molecular and cellular biochemistry
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doi:10.1007/s11010-007-9438-1
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journal_title:Molecular and cellular biochemistry
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doi:
更新日期:1998-09-01 00:00:00
abstract::Persistent activation of nuclear factor B (NF-κB) is very important in the modulation of macrophages cellular response to microbial infections. The deubiquitinase USP14, which is critical for ubiquitin-mediated proteasomal degradation of proteins, is known to be involved in cancer, neurological diseases, and aging. Ho...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/s11010-017-2978-0
更新日期:2017-07-01 00:00:00
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journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章,评审
doi:10.1007/BF01732005
更新日期:1975-02-28 00:00:00
abstract::Cofilin is an actin-binding protein of low molecular weight which is widely distributed in eukaryotes and is deeply involved in the dynamics of actin assembly in the cytoplasm. The actin-binding ability of cofilin is inhibited by inositol phosphates (PIP2), and the PIP2- and actin-binding site(s) has been localized in...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:
更新日期:1999-01-01 00:00:00
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journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/BF00223357
更新日期:1979-12-14 00:00:00
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journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/BF00573841
更新日期:1982-04-16 00:00:00
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journal_title:Molecular and cellular biochemistry
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journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/BF00227544
更新日期:1996-09-20 00:00:00
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journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1023/a:1006851426257
更新日期:1997-12-01 00:00:00
abstract::The antioxidant activity of anthocyanins has been well characterized in vitro; many cases has been postulated to provide an important exogenous mediator of oxidative stress in the gastrointestinal tract. The objective of this study was to evaluate the efficacy of anthocyanin protection against peroxyl radical (AAPH)-i...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/s11010-008-9729-1
更新日期:2008-05-01 00:00:00
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journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章,评审
doi:10.1007/BF00285225
更新日期:1984-09-01 00:00:00
abstract::In mammals, protein kinase CK2 has two isozymic forms of its catalytic subunit, designated CK2alpha and CK2alpha'. CK2alpha and CK2alpha' exhibit extensive similarity within their catalytic domains but have completely unrelated C-terminal sequences. To systematically examine the cellular functions of each CK2 isoform ...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:
更新日期:2001-11-01 00:00:00
abstract::Cancer cell aggregation has been long known to facilitate metastatic potential of cancer cells. In addition, the presence of nitric oxide (NO) in cancer area may have a significant impact on aggregation behavior of the cells. We show herein that lung cancer H460 cells possessing high ability of anoikis resistance form...
journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/s11010-014-2066-7
更新日期:2014-08-01 00:00:00
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journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/s11010-015-2353-y
更新日期:2015-05-01 00:00:00
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journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/s11010-010-0630-3
更新日期:2011-01-01 00:00:00
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journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1023/a:1007106529644
更新日期:2000-07-01 00:00:00
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journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
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journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/BF01731649
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pub_type: 杂志文章
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更新日期:2018-02-01 00:00:00
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journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/s11010-011-1131-8
更新日期:2012-03-01 00:00:00
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journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/BF00228455
更新日期:1990-07-17 00:00:00
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journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:10.1007/BF00226230
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journal_title:Molecular and cellular biochemistry
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journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
doi:
更新日期:1997-11-01 00:00:00
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journal_title:Molecular and cellular biochemistry
pub_type: 杂志文章
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更新日期:2015-10-01 00:00:00