Abstract:
:The uptake of glucose and gluconate is under inductive control in Pseudomonas putida. Glucose, gluconate, and 2-ketogluconate were each good nutritional inducers of these transport abilities. Glucose and gluconate uptake obeyed saturation kinetics: the apparent Km for glucose was 6 mM and that for gluconate was 0.5 mM. Therefore, transport of both substrates appears to be mediated by enzyme-like carriers. Glucose and gluconate are parallel inhibitors for their uptake9 Strains selected for their inability totransport glucose were found to be deficient in gluconate uptake. The reverse was alsotrue: mutations affecting gluconate entry also blocked the uptake of glucose. These results demonstrate that a common carrier is involved in the uptake of both glucose and gluconate by P. putida cells.
journal_name
Mol Cell Biochemjournal_title
Molecular and cellular biochemistryauthors
Vicente M,Pedro MA,Torrontegui G,Cánovas JLdoi
10.1007/BF01732164keywords:
subject
Has Abstractpub_date
1975-04-30 00:00:00pages
59-64issue
1eissn
0300-8177issn
1573-4919journal_volume
7pub_type
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