当前位置： SCI文献检索 > MOLECULAR AND CELLULAR BIOCHEMISTRY期刊下所有文献 > Deoxyribose degradation catalyzed by Fe(III)-EDTA: kinetic aspects and potential usefulness for submicromolar iron measurements.

Deoxyribose degradation catalyzed by Fe(III)-EDTA: kinetic aspects and potential usefulness for submicromolar iron measurements.

Abstract：

:Iron ions play a central role in .OH radicals formation and induction of oxidative stress in living organisms. Iron-catalyzed .OH radical formation degrades deoxyribose to thiobarbituric acid reactive substances (TBA-RS). This paper analyzes kinetic properties of the Fe(III)-EDTA-catalyzed deoxyribose degradation in the presence of ascorbate. The yield of TBA-RS formation in the presence of EDTA was 4-fold higher than in its absence, contrasting with results reported elsewhere, Cu(II)-EDTA and Fe(III)-citrate were unable to catalyze deoxyribose degradation. The dependence on deoxyribose concentration was fitted to a Lineweaver Burk-like plot and it was calculated that approximately 4.5 mM deoxyribose scavenged half of the .OH radicals formed. The data for Fe(III)-EDTA concentration dependence could also be fitted to a rectangular hyperbolic function. This function was linear up to 1 microM added FeCl3 and this property could be utilized as an assay for the estimation of submicromolar iron concentrations. Submicromolar concentrations of iron could induce measurable yields of TBA-RS. Differences of as little as 0.1 microM Fe(III)-EDTA could be reproducibly detected under optimum experimental conditions, above a consistent background absorbance that was equivalent to 0.35 +/- 0.05 microM Fe(III)-EDTA and represented contaminating iron in the reactants that could not be removed with Chelex-100. The low method determination limit makes the deoxyribose degradation reaction potentially useful as a new, highly sensitive and cost effective assay for iron quantification.

journal_name

Mol Cell Biochem

journal_title

Molecular and cellular biochemistry

authors

Hermes-Lima M,Wang EM,Schulman HM,Storey KB,Ponka P

doi

10.1007/BF00926041

subject

Has Abstract

pub_date

1994-08-17 00:00:00

pages

65-73

issue

1

eissn

0300-8177

issn

1573-4919

journal_volume

137

pub_type

杂志文章

相关文献

MOLECULAR AND CELLULAR BIOCHEMISTRY文献大全
  • Effect of albumin on acyl-CoA: lysolecithin acyltransferase, lysolecithin: lysolecithin acyltransferase and acyl-CoA hydrolase from rabbit lung.

    abstract：:Acyl-CoA: lysolecithin and lysolecithin: lysolecithin acyltransferases, as well as acyl-CoA hydrolase are important enzymes in lung lipid metabolism. They use amphiphylic lipids as substrates and differ in subcellular localization. In this sense, lipid-protein interactions can be an essential factor in their activity....

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/BF00214123

    authors： Pérez-Gil J,Estrada P,Acebal C,Arche R

    更新日期：1990-05-10 00:00:00

  • Arrest of cell cycle by amida which is phosphorylated by Cdc2 kinase.

    abstract：:Amida was first isolated from a rat hippocampal cDNA library as an Arc-associated protein. Although previous studies have shown that Amida mRNA is predominantly expressed and developmentally regulated in rat testis and overexpression induces apoptosis, the function of Amida remains unclear. In this study, we found tha...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：

    authors： Gan Y,Taira E,Irie Y,Fujimoto T,Miki N

    更新日期：2003-04-01 00:00:00

  • Photoreceptor specific guanylate cyclases in vertebrate phototransduction.

    abstract：:Two membrane bound guanylate cyclases are expressed in vertebrate photoreceptor cells. They serve a key function in photoreceptor physiology as they synthesize the intracellular transmitter of photoexcitation guanosine 3',5'-cyclic monophosphate (cGMP). Both cyclases named ROS-GC1 and ROS-GC2 form a subclass of membra...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章,评审

    doi：

    authors： Koch KW,Duda T,Sharma RK

    更新日期：2002-01-01 00:00:00

  • Ubiquitin-like protein MNSFβ noncovalently binds to molecular chaperone HSPA8 and regulates osteoclastogenesis.

    abstract：:MNSFβ, a ubiquitin-like protein, covalently binds to various target proteins including proapoptotic Bcl-G. During the course of isolation of MNSFβ-conjugating enzyme(s), we identified a novel target protein for MNSFβ. MALDI-TOF MS fingerprinting revealed that the MNSFβ-interacting protein is HSPA8 (heat shock 70-kDa p...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-016-2795-x

    authors： Notsu K,Nakagawa M,Nakamura M

    更新日期：2016-10-01 00:00:00

  • Dimethylesculetin ameliorates maternal glucose intolerance and fetal overgrowth in high-fat diet-fed pregnant mice via constitutive androstane receptor.

    abstract：:The constitutive androstane receptor (CAR) has been reported to decrease insulin resistance along with obesity. 6,7-dimethylesculetin (DE) is an active component of Yin Zhi Huang which is a traditional Asian medicine used to treat neonatal jaundice via CAR. In this study, we examined whether DE could affect the expres...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-016-2772-4

    authors： Masuyama H,Mitsui T,Maki J,Tani K,Nakamura K,Hiramatsu Y

    更新日期：2016-08-01 00:00:00

  • The adipokine Chemerin induces lipolysis and adipogenesis in bovine intramuscular adipocytes.

    abstract：:The adipokine Chemerin is reported to regulate adipogenesis and glucose homeostasis in vivo and in 3T3-L1 cells. Our team is focused on the role of Chemerin in metabolism and intramuscular adipocyte differentiation because intramuscular fat is the basic material for the formation of marbling in livestock and poultry m...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-016-2731-0

    authors： Fu YY,Chen KL,Li HX,Zhou GH

    更新日期：2016-07-01 00:00:00

  • A simple method for the simultaneous isolation of stellate cells and hepatocytes from rat liver tissue.

    abstract：:Hepatic stellate cells (HSCs), also referred to as Ito cells, perisinusiodal cells and fat-storing cells, have numerous vital functions. They are the main extracellular matrix-producing cells within the liver and are involved in the storage of retinol. HSCs are also known to secrete a number of liver mitogens. Current...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1023/a:1024184826728

    authors： Riccalton-Banks L,Bhandari R,Fry J,Shakesheff KM

    更新日期：2003-06-01 00:00:00

  • Semecarpus anacardium Linn. nut milk extract, an indigenous drug preparation, modulates reactive oxygen/nitrogen species levels and antioxidative system in adjuvant arthritic rats.

    abstract：:Reactive oxygen species (ROS) and reactive nitrogen species (RNS) are highly reactive transient chemical species, which play an important role in the etiology of tissue injury in rheumatoid arthritis (RA). The effects of milk extract of Semecarpus anacardium Linn. nut (SA) was studied on adjuvant arthritis in rats. Ar...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-005-3202-1

    authors： Ramprasath VR,Shanthi P,Sachdanandam P

    更新日期：2005-08-01 00:00:00

  • Circular RNA circ_HN1 facilitates gastric cancer progression through modulation of the miR-302b-3p/ROCK2 axis.

    abstract：:Gastric cancer (GC) is a malignant tumor with high morbidity and mortality in the world. Circular RNA hsa_circHN1_005 (circ_HN1), also termed as hsa_circ_0045602, is reported as an oncogene in GC. However, the molecular mechanism of circ_HN1 in GC development has not been fully explored. Here, we surveyed the regulato...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-020-03897-2

    authors： Wang D,Jiang X,Liu Y,Cao G,Zhang X,Kuang Y

    更新日期：2020-09-19 00:00:00

  • Magnetite induces oxidative stress and apoptosis in lung epithelial cells.

    abstract：:There is an ongoing concern regarding the biocompatibility of nanoparticles with sizes less than 100 nm as compared to larger particles of the same nominal substance. In this study, we investigated the toxic properties of magnetite stabilized with polyacrylate sodium. The magnetite was characterized by X-ray powder di...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-011-1174-x

    authors： Ramesh V,Ravichandran P,Copeland CL,Gopikrishnan R,Biradar S,Goornavar V,Ramesh GT,Hall JC

    更新日期：2012-04-01 00:00:00

  • Effect of chronic colchicine administration on the myocardium of the aging spontaneously hypertensive rat.

    abstract：:Colchicine has been demonstrated to suppress the release of fibroblast growth factors, retard collagen formation and augment collagenase activity. Trials with colchicine in patients with hepatic fibrosis have suggested clinical benefit. The development of impaired myocardial function in the spontaneously hypertensive ...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1023/a:1006889126666

    authors： Cicogna AC,Brooks WW,Hayes JA,Robinson KG,Sen S,Conrad CH,Bing OH

    更新日期：1997-01-01 00:00:00

  • Nitric oxide mediates cell aggregation and mesenchymal to epithelial transition in anoikis-resistant lung cancer cells.

    abstract：:Cancer cell aggregation has been long known to facilitate metastatic potential of cancer cells. In addition, the presence of nitric oxide (NO) in cancer area may have a significant impact on aggregation behavior of the cells. We show herein that lung cancer H460 cells possessing high ability of anoikis resistance form...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-014-2066-7

    authors： Powan P,Chanvorachote P

    更新日期：2014-08-01 00:00:00

  • Alterations in human gingival glycosaminoglycan pattern in inflammation and in phenytoin induced overgrowth.

    abstract：:Glycosaminoglycans were extracted from normal, inflamed and phenytoin induced overgrowth of human gingival tissue by proteolysis and alcohol precipitation. Extracts were run in a Dowex-1 column and the fractions were treated with mucopolysaccharidases. Cellulose acetate electrophoresis was carried out with or without ...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/BF00230325

    authors： Suresh R,Puvanakrishnan R,Dhar SC

    更新日期：1992-10-07 00:00:00

  • Increased expression of IL-6 and LIF in the hypertrophied left ventricle of TGR(mRen2)27 and SHR rats.

    abstract：:Cytokines from the interleukin-6 (IL-6) family have been reported to play an important synergistic role with angiotensin II in the development of pathological cardiac hypertrophy. Whether their expression pattern changes in vivo, in an angiotensin I-dependent hypertrophied myocardium has not been reported. In this stu...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-005-3085-1

    authors： Kurdi M,Randon J,Cerutti C,Bricca G

    更新日期：2005-01-01 00:00:00

  • Effect of 60 Hz magnetic field exposure on c-fos expression in stimulated PC12 cells.

    abstract：:Rat pheochromocytoma PC12 cells have been treated with nerve growth factor (NGF) at final concentrations of 2, 4, 8, and 16 ng/ml, and then were exposed to 60-Hz, sinusoidal magnetic fields (MF) of 12.5, 25, 50, and 100 microT (rms) for 30 min. Transcript levels for both c-fos and glyceraldehyde-3 -phosphate dehydroge...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1023/a:1006872309385

    authors： Campbell-Beachler M,Ishida-Jones T,Haggren W,Phillips JL

    更新日期：1998-12-01 00:00:00

  • Inhibition of nitric oxide synthase enhances contractile response of ventricular myocytes from streptozotocin-diabetic rats.

    abstract：:The contractile hyporesponsiveness of the streptozotocin diabetic rat heart in vitro to beta-adrenergic agonists is eliminated when the heart is perfused with N(G)-nitro-L-arginine methyl ester (L-NAME), a non-selective inhibitor of nitric oxide synthase (NOS). The following study evaluated the hypothesis that an incr...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-006-9376-3

    authors： Smith JM,Sondgeroth KB,Wahler GM

    更新日期：2007-06-01 00:00:00

  • Identification of an atypical form of 30 kDa SOD--a possible virulence factor in clinical isolates of Shigella spp.

    abstract：:Reviews on the pathogenic mechanisms of Shigella species show a lacunae in the understanding of the bacterial antioxidant defense system and its regulations. This study was done to investigate the regulation of expression of antioxidant enzymes in clinical isolates of Shigella species, under various growth conditions....

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1023/b:mcbi.0000049369.78195.b1

    authors： Prakash R,Devaraj H,Devaraj SN

    更新日期：2004-12-01 00:00:00

  • Identification of an autoinhibitory domain in the insulin receptor tyrosine kinase.

    abstract：:We have tested the hypothesis that activation of the insulin receptor tyrosine kinase is due to autophosphorylation of tyrosines 1146, 1150 and 1151 within a putative autoinhibitory domain. A synthetic peptide corresponding to residues 1134-1162, with tyrosines substituted by alanine or phenylalanine, of the insulin r...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/BF00926082

    authors： Filipek A,Soderling TR

    更新日期：1993-03-24 00:00:00

  • HMGb1 promotes scratch wound closure of HaCaT keratinocytes via ERK1/2 activation.

    abstract：:HMGb1 is a DNA-binding protein whose role as an extracellular cytokine in inflammation and tissue regeneration has also been reported. Given the importance of keratinocytes in wound healing, we have studied the mechanism of action of HMGb1 on HaCaT keratinocytes during in vitro scratch wound repair. Western blot and c...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-009-0192-4

    authors： Ranzato E,Patrone M,Pedrazzi M,Burlando B

    更新日期：2009-12-01 00:00:00

  • Troxerutin suppresses lipid abnormalities in the heart of high-fat-high-fructose diet-fed mice.

    abstract：:The reversal effect of troxerutin (TX) on obesity, insulin resistance, lipid accumulation, oxidative damage, and hypertension induced in the high-fat-high-fructose diet (HFFD)-fed mice model of metabolic syndrome was investigated. Adult male Mus musculus mice of body weight 25-30 g were fed either control diet or HFFD...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-013-1877-2

    authors： Geetha R,Yogalakshmi B,Sreeja S,Bhavani K,Anuradha CV

    更新日期：2014-02-01 00:00:00

  • Peroxisome distribution along the crypt-villus axis of the guinea pig small intestine.

    abstract：:Peroxisomes and peroxisomal enzyme expression were investigated biochemically and morphometrically in guinea pig intestinal epithelial cells at different stages of their migration along the crypt-villus axis. Epithelial cells were sequentially isolated along the axis and the specific activities of the peroxisomal enzy...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1023/a:1007052003143

    authors： Phipps AN,Connock MJ,Johnson P,Burdett K

    更新日期：2000-01-01 00:00:00

  • The role of Ca2+ mobilization and heterotrimeric G protein activation in mediating tyrosine phosphorylation signaling patterns in vascular smooth muscle cells.

    abstract：:This work investigated the role of Ca2+ mobilization and heterotrimeric G protein activation in mediating angiotensin II-dependent tyrosine phosphorylation signaling patterns. We demonstrate that the predominant, angiotensin II-dependent, tyrosine phosphorylation signaling patterns seen in vascular smooth muscle cells...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：

    authors： Sayeski PP,Ali MS,Bernstein KE

    更新日期：2000-09-01 00:00:00

  • The NADPH oxidase of guinea pig polymorphonuclear leucocytes. Properties of the deoxycholate extracted enzyme.

    abstract：:NADPH oxidase from stimulated guinea pig granulocytes was extracted with deoxycholate. The solubilized enzyme was stable in 20% glycerol. Solubilized enzyme was free of myeloperoxidase activity. The properties of the deoxycholate solubilized enzyme indicated that it is a high molecular weight complex with a flavoprote...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/BF00230585

    authors： Bellavite P,Serra MC,Davoli A,Bannister JV,Rossi F

    更新日期：1983-01-01 00:00:00

  • Oxidation and methylation status determine the effects of arsenic on the mitotic apparatus.

    abstract：:We investigated the spindle inhibitory properties of six arsenicals differing in their methylation or oxidation state. Human lymphoblasts were exposed for 6 h to either sodium arsenate (NaAs(V)), sodium arsenite (NaAs(III)), monomethylarsonic acid (MMA(V)), monomethylarsonous acid (MMA(III)), dimethylarsinic acid (DMA...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-005-8283-3

    authors： Kligerman AD,Doerr CL,Tennant AH

    更新日期：2005-11-01 00:00:00

  • miR-202 suppresses proliferation and induces apoptosis of osteosarcoma cells by downregulating Gli2.

    abstract：:Accumulating data have shown that microRNAs are involved in the pathogenesis of cancer. miR-202 has been confirmed to be downregulated in several types of human cancer. However, the expression and biological role of miR-202 in osteosarcoma (OS) carcinogenesis and progression remain unclear. In this study, we demonstra...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-014-2195-z

    authors： Sun Z,Zhang T,Hong H,Liu Q,Zhang H

    更新日期：2014-12-01 00:00:00

  • Microassay for malignant hyperthermia susceptibility: hypersensitive ligand-gating of the Ca channel in muscle sarcoplasmic reticulum causes increased amounts and rates of Ca-release.

    abstract：:A crude preparation of heavy sarcoplasmic reticulum (HSR) was isolated using 1 gram of muscle obtained from swine susceptible to malignant hyperthermia (MH) and from control swine. The caffeine and ATP concentration-dependency of Ca-release was determined using suction filtration with radioisotopic 45Ca as a tracer. R...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/BF00223492

    authors： O'Brien PJ

    更新日期：1990-03-05 00:00:00

  • Effect of adjuvants on immune response and protective immunity elicited by recombinant Hsp60 (GroEL) of Salmonella typhi against S. typhi infection.

    abstract：:Heat shock proteins (Hsps) have been reported to be dominant antigens for the host immune response to various pathogens and thus, have great potential for use in vaccination. In the present study, we evaluated the immunogenicity and protective efficacy of GroEL of Salmonella enterica serovar Typhi against lethal infec...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-009-0301-4

    authors： Bansal A,Paliwal PK,Sagi SS,Sairam M

    更新日期：2010-04-01 00:00:00

  • Effect of phospholipids on the activity of sialosyl lactosylceramide (GM3): N-acetylgalactosaminyl transferase from chick embryo brain.

    abstract：:A preparation of UDP-GalNAc:sialosyl lactosylceramide N-acetylgalactosaminyl transferase (E.C. 2.4.1.92) obtained from 14-day chick embryo brain was delipidated partially by treatment with cold acetone in the presence of varying amounts of sodium dodecyl sulphate (SDS). The lipid content and the enzyme activity of the...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/BF00223509

    authors： Cortassa S,Maccioni HJ

    更新日期：1989-01-23 00:00:00

  • MicroRNAs: novel biomarkers for gastrointestinal carcinomas.

    abstract：:MicroRNAs (miRNAs) are a class of non-coding small RNAs. They play an important role as a post-transcriptional regulator in the protein synthesis and are involved in cellular processes, such as proliferation, apoptosis, and differentiation. miRNAs give us a new perspective to understand carcinogenesis. Since the high-...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章,评审

    doi：10.1007/s11010-010-0463-0

    authors： Xie L,Qian X,Liu B

    更新日期：2010-08-01 00:00:00

  • Endogenous antioxidant changes in the myocardium in response to acute and chronic stress conditions.

    abstract：:Oxygen is a diradical and because of its unique electronic configuration, it has the potential to form strong oxidants (e.g. superoxide radical, hydrogen peroxide and hydroxyl radical) called oxygen free radicals or partially reduced forms of oxygen (PRFO). These highly reactive oxygen species can cause cellular injur...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章,评审

    doi：10.1007/BF00926366

    authors： Singal PK,Dhalla AK,Hill M,Thomas TP

    更新日期：1993-12-22 00:00:00