Abstract：
:Reviews on the pathogenic mechanisms of Shigella species show a lacunae in the understanding of the bacterial antioxidant defense system and its regulations. This study was done to investigate the regulation of expression of antioxidant enzymes in clinical isolates of Shigella species, under various growth conditions. The in vitro expression of superoxide dismutase in the clinical isolates of Shigella spp., is modulated by both endogenous and exogenous factors. During aerobic and iron repleted growth conditions, the expression of the MnSOD and FeSOD enzymes were higher, and an atypical SOD was also expressed. However, under anaerobic growth conditions and in plasmid-cured strains, the antioxidant enzyme activities were decreased and the atypical SOD was not expressed. Absence of the atypical form of SOD may be due to the low oxygen environment. Plasmid-encoded factors may also play a role in the expression of this SOD, which had a molecular weight of approximately 30 kDa. In the rat ileal loop ligation assay, mild lesions were observed only in the intestinal microvilli of rats injected with plasmid-cured strains of Shigella spp., suggesting that plasmid-encoded factors, including those that regulate the expression of the atypical SOD, are essential for the virulence of Shigella spp.
journal_name
Mol Cell Biochemjournal_title
Molecular and cellular biochemistryauthors
Prakash R,Devaraj H,Devaraj SNdoi
10.1023/b:mcbi.0000049369.78195.b1keywords:
subject
Has Abstractpub_date
2004-12-01 00:00:00pages
91-8issue
1-2eissn
0300-8177issn
1573-4919journal_volume
267pub_type
杂志文章abstract：:We have tested the hypothesis that activation of the insulin receptor tyrosine kinase is due to autophosphorylation of tyrosines 1146, 1150 and 1151 within a putative autoinhibitory domain. A synthetic peptide corresponding to residues 1134-1162, with tyrosines substituted by alanine or phenylalanine, of the insulin r...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
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pub_type： 杂志文章
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更新日期：2014-09-01 00:00:00
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
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pub_type： 杂志文章,评审
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更新日期：2020-12-01 00:00:00
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doi：10.1007/s11010-005-3202-1
更新日期：2005-08-01 00:00:00
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doi：10.1007/BF02354825
更新日期：1981-03-13 00:00:00
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pub_type： 杂志文章
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更新日期：2005-02-01 00:00:00
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/BF00231384
更新日期：1990-10-15 00:00:00
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pub_type： 杂志文章,评审
doi：10.1007/s11010-010-0463-0
更新日期：2010-08-01 00:00:00
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pub_type： 杂志文章
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更新日期：2012-06-01 00:00:00
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journal_title：Molecular and cellular biochemistry
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更新日期：2002-10-01 00:00:00
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更新日期：1997-11-01 00:00:00
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