Abstract：
:Chromium(VI) [Cr(VI)] induces chronic inflammation in hepatocytes. Inflammation has been shown to play an important role in tumorigenesis, tumor progression, and metastasis. To examine the effects of the Styrax Japonica Siebold et al. Zuccarini (SJSZ) glycoprotein on inflammation in BNL CL.2 cells, we evaluated the activities of c-Jun NH(2)-terminal kinase (JNK), extracellular signal-regulated kinase (ERK), nuclear factor (NF)-κB (p50 and p65), and inflammation-related factors [cyclooxygenase (COX)-2, inducible nitric oxide syntheses (iNOS) and interleukin (IL)-1β] in Cr-induced BNL CL.2 cells using immunoblot analysis and RT-PCR. We also used two-dimensional gel electrophoresis (2-DE) to compare between treatments. To determine which proteins are induced by Cr(VI), we evaluated total protein lysates using 2-DE. After Cr(VI) treatment, total protein lysates were prepared and resolved by 2-DE. The results obtained from this study demonstrated that the SJSZ glycoprotein (50 μg/ml) inhibits expression of JNK, ERK, NF-κB, and the expression of COX-2, iNOS, and IL-1β. Moreover, the results obtained from 2DE showed that four proteins out of nine proteins were relatively expressed strongly, while the rest of them were relatively appeared weakly on the gel. Taken together, these data indicate that the SJSZ glycoprotein prevents expression of COX-2, iNOS, and IL-1β by blocking NF-κB and MAPKs in Cr(VI)-induced BNL CL.2 cells.
journal_name
Mol Cell Biochemjournal_title
Molecular and cellular biochemistryauthors
Lee J,Lim KTdoi
10.1007/s11010-012-1324-9subject
Has Abstractpub_date
2012-08-01 00:00:00pages
103-11issue
1-2eissn
0300-8177issn
1573-4919journal_volume
367pub_type
杂志文章abstract：:Understanding the driving forces for the hepatic uptake of endogenous and exogenous substrates in isolated cells and organs is fundamental to describing the underlying hepatic physiology/pharmacology. In this study we investigated whether uptake of plasma protein-bound [3H]-palmitate across the hepatocyte wall is gove...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
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更新日期：2010-04-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s11010-016-2907-7
更新日期：2017-03-01 00:00:00
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更新日期：2016-05-01 00:00:00
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doi：10.1007/s11010-013-1706-7
更新日期：2013-09-01 00:00:00
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更新日期：1998-09-01 00:00:00
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pub_type： 杂志文章,评审
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journal_title：Molecular and cellular biochemistry
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更新日期：1996-01-12 00:00:00
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journal_title：Molecular and cellular biochemistry
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