Abstract:
:Phospholipase C-beta (PLCbeta) isozymes (PLCbeta(1) and PLCbeta(3)) have been extensively characterized in cardiac tissue, but no data are available for the PLCbeta(4) isozyme. In this study, PLCbeta((1-4)) isozymes mRNA relative expression was studied by real-time PCR (RT-PCR) in human, rat, and murine left ventricle and the presence of PLCbeta(4) isozyme at the protein level was confirmed by Western blotting in all species studied. Confocal microscopy experiments carried out in HL-1 cardiomyocytes revealed a sarcoplasmic subcellular distribution of PLCbeta(4). Although there were unexpected significant interspecies differences in the PLCbeta((1-4)) mRNA expression, PLCbeta(4) mRNA was the main transcript expressed in all left ventricles studied. Thus, whereas in human and rat left ventricles PLCbeta(4) > PLCbeta(3) > PLCbeta(2) > PLCbeta(1) mRNA pattern of expression was found, in murine left ventricle the pattern of expression was different, i.e., PLCbeta(4) > PLCbeta(1) > PLCbeta(3) > PLCbeta(2). However, results obtained in mouse HL-1 cardiomyocytes showed PLCbeta(3) approximately PLCbeta(4) > PLCbeta(1) > PLCbeta(2) pattern of mRNA expression indicating a probable cell type specific expression of the different PLCbeta isozymes in cardiomyocytes. Finally, RT-PCR experiments showed a trend, even though not significant (P = 0.067), to increase PLCbeta(4) mRNA levels in HL-1 cardiomyocytes after angiotensin II treatment. These results demonstrate the presence of PLCbeta(4) in the heart and in HL-1 cardiomyocytes showing a different species-dependent pattern of expression of the PLCbeta((1-4)) transcripts. We discuss the relevance of these findings in relation to the development of cardiac hypertrophy.
journal_name
Mol Cell Biochemjournal_title
Molecular and cellular biochemistryauthors
Otaegui D,Querejeta R,Arrieta A,Lazkano A,Bidaurrazaga A,Arriandiaga JR,Aldazabal P,Garro MAdoi
10.1007/s11010-009-0296-xsubject
Has Abstractpub_date
2010-04-01 00:00:00pages
167-73issue
1-2eissn
0300-8177issn
1573-4919journal_volume
337pub_type
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